Expression of larval jelly antimicrobial peptide defensin1 in Apis mellifera colonies

Klaudiny, Jaroslav; Bachanová, Katarína; Kohútová, Lenka; Dzúrovä, Mária; Kopernický, Ján; Majtán, Juraj

doi:10.2478/s11756-011-0153-8

Cited by 23 publications

(17 citation statements)

References 70 publications

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“…However, bee Def-1 significantly enhanced the secretion of MMP-9 from keratinocytes. Similar to MRJP1, Def-1 is a common but quantitatively variable factor present in both RJ and honey 8 , 28 . The concentration of Def-1 in RJ and honey varied in amounts ranging from 0.159 to 0.524 µg/mg 8 and 0.04 to 5.17 µg/g 28 , respectively.…”

Section: Discussionmentioning

confidence: 97%

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Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo

Bucekova

Sojka

Valachová

et al. 2017

Sci Rep

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Royal jelly (RJ) has successfully been used as a remedy in wound healing. RJ has multiple effects, including antibacterial, anti-inflammatory and immunomodulatory activities, in various cell types. However, no component(s) (other than antibacterial) have been identified in RJ-accelerated wound healing. In this study, we demonstrate that keratinocytes are responsible for the elevated production of matrix metalloproteinase-9 (MMP-9) after incubation with a water extract of RJ. Furthermore, the keratinocyte migration and wound closure rates were significantly increased in the presence of RJ extract. MMP-9 production was reduced significantly following proteinase K treatment but remained stable after heat treatment, indicating that active component(s) have a proteinous character. To identify the component responsible for inducing MMP-9 production, RJ extract was fractionated using C18 RP-HPLC. In fractions exhibiting stimulatory activity, we immunochemically detected the bee-derived antibacterial peptide, defensin-1. Defensin-1 was cloned, and recombinant peptide was produced in a baculoviral expression system. Defensin-1 stimulated MMP-9 secretion from keratinocytes and increased keratinocyte migration and wound closure in vitro. In addition, defensin-1 promoted re-epithelisation and wound closure in uninfected excision wounds. These data indisputably demonstrate that defensin-1, a regular but concentration variable factor found in honey and RJ, contributes to cutaneous wound closure by enhancing keratinocyte migration and MMP-9 secretion.

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Section: Discussionmentioning

confidence: 97%

“…RJ has been used since ancient times to facilitate wound healing. RJ acts as an antimicrobial 4 – 8 and antioxidative agent 9 and as an immunomodulator with anti-inflammatory properties 10 , 11 .…”

Section: Introductionmentioning

confidence: 99%

Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo

Bucekova

Sojka

Valachová

et al. 2017

Sci Rep

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“…Recently, defensin1, one of the major antibacterial factors in honey, was shown to be a regular but quantitatively variable component of each honey . One reason for the varying contents of defensin1 in different honeys seems to be constitutive but variable defensin1 expression in individual honeybees in bee populations . It has also been found that some types of honey derived from specific floral sources become more potent than others because of the presence of phytochemicals with antibacterial properties .…”

Section: Immumodulatory Compounds Of Various Honey Samples and Their mentioning

confidence: 99%

“…2 One reason for the varying contents of defensin1 in different honeys seems to be constitutive but variable defensin1 expression in individual honeybees in bee populations. 3 It has also been found that some types of honey derived from specific floral sources become more potent than others because of the presence of phytochemicals with antibacterial properties. [4][5][6] These potent natural honeys, such as manuka (Medihoney, Comvita NZ Ltd., Te Puke, New Zealand) and RS honey (Bfactory Health Products B.V., Rhenen, The Netherlands) (honey with unknown origin used as a source for Revamil), are currently being used as medical-grade honeys in clinical applications.…”

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confidence: 99%

Honey: An immunomodulator in wound healing

Majtán

2014

Wound Repair Regeneration

165

105

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Honey is a popular natural product that is used in the treatment of burns and a broad spectrum of injuries, in particular chronic wounds. The antibacterial potential of honey has been considered the exclusive criterion for its wound healing properties. The antibacterial activity of honey has recently been fully characterized in medical-grade honeys. Recently, the multifunctional immunomodulatory properties of honey have attracted much attention. The aim of this review is to provide closer insight into the potential immunomodulatory effects of honey in wound healing. Honey and its components are able to either stimulate or inhibit the release of certain cytokines (tumor necrosis factor-α, interleukin-1β, interleukin-6) from human monocytes and macrophages, depending on wound condition. Similarly, honey seems to either reduce or activate the production of reactive oxygen species from neutrophils, also depending on the wound microenvironment. The honey-induced activation of both types of immune cells could promote debridement of a wound and speed up the repair process. Similarly, human keratinocytes, fibroblasts, and endothelial cell responses (e.g., cell migration and proliferation, collagen matrix production, chemotaxis) are positively affected in the presence of honey; thus, honey may accelerate reepithelization and wound closure. The immunomodulatory activity of honey is highly complex because of the involvement of multiple quantitatively variable compounds among honeys of different origins. The identification of these individual compounds and their contributions to wound healing is crucial for a better understanding of the mechanisms behind honey-mediated healing of chronic wounds.

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“…Both nurses and foragers produce antimicrobial peptide defensin1 (Liu et al 2011;Klaudiny et al 2012;Majtan et al 2012). Nurses synthesize the peptide in their hypopharyngeal and mandibular glands (located in head) from which it is secreted into larval food -larval jelly (LJ) (Klaudiny et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Identification and validation of reference genes for real time PCR expression studies in heads of nurse honeybees

et al. 2013

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Abstract:The primary aim of this study was to identify reference genes and workers of particular role and ages that would be suitable for exploring genetic/epigenetic variations in constitutive expression of a gene encoding antimicrobial peptide defensin1 in worker heads using real-time PCR. This peptide is an integral component of larval food and honey and has potential to act against some brood pathogens. Expression levels of distinct genes may vary in worker heads due to genetic factors, age of bee, and particular role of a worker that depends on its age or colony needs. Prerequisite for exploring the variations in defensin1 expression was therefore to identify such workers in which correlated expression of defensin1 and suitable reference genes occurs. Selection process was done by carefully designed quantitative real-time PCR procedure in two colonies showing different age-related division of labor. Expression of ten candidate reference genes, defensin1 and amylase, as a marker of forager bees, was assessed in pooled head samples of workers aged 2 to 30 days. Correlated and moreover stable expression of defensin1 and six candidate genes was detected in nursing bees in both colonies. The suitable reference genes were therefore selected on the basis of their expression stability. This was evaluated by geNorm and NormFinder algorithms in pooled head samples and through plotted Cq data in head samples of individual nurse bees. As the best reference genes were selected: psa1, tctp1, cyclophilin, gapdh and mrjp4 (in this order). They are suitable for aforementioned defensin1 expression studies and also for studies of other genes expressed in heads of nurses. In addition, an amylase expression-based procedure for reliable distinguishing nurses from foragers was elaborated.

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Expression of larval jelly antimicrobial peptide defensin1 in Apis mellifera colonies

Cited by 23 publications

References 70 publications

Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo

Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo

Honey: An immunomodulator in wound healing

Identification and validation of reference genes for real time PCR expression studies in heads of nurse honeybees

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