Expression of lavender AGAMOUS-like and SEPALLATA3-like genes promote early flowering and alter leaf morphology in Arabidopsis thaliana

Adal, Ayelign M.; Binson, Elinor; Remedios, Lisa; Mahmoud, Soheil S.

doi:10.1007/s00425-021-03703-3

Cited by 12 publications

(9 citation statements)

References 32 publications

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“…Our overexpression experiments provided a direct assessment of molecular functions of IlSEP3 and IlSVP , which showed that IlSEP3 promotes flowering. This is consistent with previous studies in which an earlier flower phenotype was observed in transgenic Arabidopsis overexpressing SEP3 from different plant species including lavender [ 35 ], Ziziphus jujuba Mill. [ 36 ], lily [ 37 ], and woad ( Isatis indigotica ) [ 38 ].…”

Section: Discussionsupporting

confidence: 93%

Identification of MADS-Box Transcription Factors in Iris laevigata and Functional Assessment of IlSEP3 and IlSVP during Flowering

Liu

Shi

et al. 2022

IJMS

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Iris laevigata is ideal for gardening and landscaping in northeast China because of its beautiful flowers and strong cold resistance. However, the short length of flowering time (2 days for individual flowers) greatly limits its applications. Molecular breeding and engineering hold high potential for producing I. laevigata of desirable flowering properties. A prerequisite is to identify and characterize key flowering control genes, the identity of which remains largely unknown in I. laevigata due to the lack of genome information. To fill this knowledge gap, we used sequencing data of the I. laevigata transcriptome to identify MADS-box gene-encoding transcription factors that have been shown to play key roles in developmental processes, including flowering. Our data revealed 41 putative MADS-box genes, which consisted of 8 type I (5 Mα and 3 Mβ, respectively) and 33 type II members (2 MIKC* and 31 MIKCC, respectively). We then selected IlSEP3 and IlSVP for functional studies and found that both are localized to the nucleus and that they interact physically in vitro. Ectopic expression of IlSEP3 in Arabidopsis resulted in early flowering (32 days) compared to that of control plants (36 days), which could be mediated by modulating the expression of FT, SOC1, AP1, SVP, SPL3, VRN1, and GA20OX. By contrast, plants overexpressing IlSVP were phenotypically similar to that of wild type. Our functional validation of IlSEP3 was consistent with the notion that SEP3 promotes flowering in multiple plant species and indicated that IlSEP3 regulates flowering in I. laevigata. Taken together, this work provided a systematic identification of MADS-box genes in I. laevigata and demonstrated that the flowering time of I. laevigata can be genetically controlled by altering the expression of key MADS-box genes.

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Section: Discussionsupporting

confidence: 93%

Identification of MADS-Box Transcription Factors in Iris laevigata and Functional Assessment of IlSEP3 and IlSVP during Flowering

Liu

Shi

et al. 2022

IJMS

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“…In this research, the PhSEP genes displayed differential spatial expression patterns in vegetative and reproductive tissues of P. henryanum . PhSEP genes were collectively expressed in all flower organs, as observed earlier in other plants ( Xu et al, 2006 ; Pan et al, 2014 ; Adal et al, 2021 ). The expression levels of PhSEP genes in roots and leaves were negligible.…”

Section: Discussionsupporting

confidence: 79%

“…The highly specialized and diverse morphology of flowers in orchids makes them excellent models for examining the complex network of regulatory genes involved in floral morphogenesis ( Pan et al, 2011 ). Recently, SEP -like genes have been identified and characterized in a wide range of eudicots and monocots, including Arabidopsis, rice, and orchid ( Pu et al, 2020 ; Adal et al, 2021 ; Zhu et al, 2022 ). Numerous reports have shown that these genes are instrumental in the floral evolution of diverse plants and play fundamental roles in floral organ fate determination during development by interacting with other MADS-box gene products, such as those from A-, B-, and C-class genes ( Malcomber and Kellogg, 2005 ; Mitoma and Kanno, 2018 ; Qi et al, 2020 ; Pu and Xu, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies reported that the greenish flower phenotype of Habenaria radiata (Orchidaceae) is caused by a mutation in the SEP -like MADS-box gene HrSEP-1 ( Mitoma and Kanno, 2018 ). In Lavandula angustifolia , the expression of lavender SEP -like genes promote early flowering and alter leaf morphology in A. thaliana ( Adal et al, 2021 ). In Apostasia shenzhenica , the adaxial petal does not differentiate into a specialized lip due to the loss of class B-AP3 and E-class genes ( Zhang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Three SEPALLATA-Like MADS-Box Genes Associated With Floral Development in Paphiopedilum henryanum (Orchidaceae)

et al. 2022

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Paphiopedilum (Orchidaceae) is one of the world’s most popular orchids that is found in tropical and subtropical forests and has an enormous ornamental value. SEPALLATA-like (SEP-like) MADS-box genes are responsible for floral organ specification. In this study, three SEP-like MADS-box genes, PhSEP1, PhSEP2, and PhSEP3, were identified in Paphiopedilum henryanum. These genes were 732–916 bp, with conserved SEPI and SEPII motifs. Phylogenetic analysis revealed that PhSEP genes were evolutionarily closer to the core eudicot SEP3 lineage, whereas none of them belonged to core eudicot SEP1/2/4 clades. PhSEP genes displayed non-ubiquitous expression, which was detectable across all floral organs at all developmental stages of the flower buds. Furthermore, subcellular localization experiments revealed the localization of PhSEP proteins in the nucleus. Yeast two-hybrid assays revealed no self-activation of PhSEPs. The protein–protein interactions revealed that PhSEPs possibly interact with B-class DEFICIENS-like and E-class MADS-box proteins. Our study suggests that the three SEP-like genes may play key roles in flower development in P. henryanum, which will improve our understanding of the roles of the SEP-like MADS-box gene family and provide crucial insights into the mechanisms underlying floral development in orchids.

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“…Thereafter, we also identified some important homologous genes of key traits reported in other species. MADS transcriptional regulator genes, AtSEP3 and AtSOC1, were involved in the clock output process of the photoperiodic pathway, where AtSEP3 is a downstream gene of FLOWERING LOCUS T (FT) and AtSOC1 is a flowering integrator (Hwan Lee et al, 2012;Adal et al, 2021;Fukazawa et al, 2021). Coincidentally, their homolog genes in Luffa, Lac01g012970 and Lac01g012980, were located in our QTL of daily flowering time, which may be the most likely candidate genes for further study.…”

Section: The First High-density Genetic Map Across Interspecies Of Luffamentioning

confidence: 74%

QTL mapping reveals candidate genes for main agronomic traits in Luffa based on a high-resolution genetic map

Liu¹,

Gan

Luo³

et al. 2022

Front. Plant Sci.

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Luffa is an important medicinal and edible vegetable crop of Cucurbitaceae. Strong heterosis effects and strikingly complementary characteristics were found between the two domesticated Luffa cultivars, Luffa acutangula and Luffa cylindrica. To explore the genetic basis underlying their important agronomic traits, we constructed the first interspecific high-density genetic linkage map using a BC1 population of 110 lines derived from a cross between S1174 (Luffa acutangula) and P93075 (Luffa cylindrica). The map spanned a total of 2246.74 cM with an average distance of 0.48 cM between adjacent markers. Thereafter, a large-scale field-based quantitative trait loci (QTLs) mapping was conducted for 25 important agronomic traits and 40 significant genetic loci distributed across 11 chromosomes were detected. Notably, a vital QTL (qID2) located on chromosome 9 with a minimum distance of 23 kb was identified to be responsible for the internode diameter and explained 11% of the phenotypic variation. Lac09g006860 (LacCRWN3), encoding a nuclear lamina protein involved in the control of nuclear morphology, was the only gene harbored in qID2. Sequence alignment showed completely different promoter sequences between the two parental alleles of LacCRWN3 except for some nonsynonymous single nucleotide polymorphisms (SNPs) in exons, and the expression level in thick-stem P93075 was distinctively higher than that in thin-stem S1174. According to the natural variation analysis of a population of 183 inbred lines, two main haplotypes were found for LacCRWN3: the P93075-like and S1174-like, with the former haplotype lines exhibiting significantly thicker internode diameters than those of the latter haplotype lines. It showed that LacCRWN3, as the only CRWN3 gene in Cucurbitaceae, was the most likely candidate gene regulating the internode diameter of Luffa. Our findings will be beneficial for deciphering the molecular mechanism of key phenotypic traits and promoting maker-assisted breeding in Luffa.

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Expression of lavender AGAMOUS-like and SEPALLATA3-like genes promote early flowering and alter leaf morphology in Arabidopsis thaliana

Cited by 12 publications

References 32 publications

Identification of MADS-Box Transcription Factors in Iris laevigata and Functional Assessment of IlSEP3 and IlSVP during Flowering

Identification of MADS-Box Transcription Factors in Iris laevigata and Functional Assessment of IlSEP3 and IlSVP during Flowering

Characterization of Three SEPALLATA-Like MADS-Box Genes Associated With Floral Development in Paphiopedilum henryanum (Orchidaceae)

QTL mapping reveals candidate genes for main agronomic traits in Luffa based on a high-resolution genetic map

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