2018
DOI: 10.3390/ijms19020616
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Expression of Lectins in Heterologous Systems

Abstract: Lectins are proteins that have the ability to recognize and bind in a reversible and specific way to free carbohydrates or glycoconjugates of cell membranes. For these reasons, they have been extensively used in a wide range of industrial and pharmacological applications. Currently, there is great interest in their production on a large scale. Unfortunately, conventional techniques do not provide the appropriate platform for this purpose and therefore, the heterologous production of lectins in different organi… Show more

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Cited by 27 publications
(18 citation statements)
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“…Additionally, the easiest and most efficient way to produce lectins is to exploit heterologous systems, such as bacteria and yeast. Indeed, different synthesis strategies exploit genetic engineering to transfer the genes encoding the lectin of interest to microorganisms, which can be grown in vast quantities, or to virus vectors in order to allow the synthesis of the lectins directly inside tumor cells [26,155,156]. As presented above, the latter approach has been exploited adequately for several lectins and with considerable results.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Additionally, the easiest and most efficient way to produce lectins is to exploit heterologous systems, such as bacteria and yeast. Indeed, different synthesis strategies exploit genetic engineering to transfer the genes encoding the lectin of interest to microorganisms, which can be grown in vast quantities, or to virus vectors in order to allow the synthesis of the lectins directly inside tumor cells [26,155,156]. As presented above, the latter approach has been exploited adequately for several lectins and with considerable results.…”
Section: Discussionmentioning
confidence: 99%
“…Lectins can be classified using different criteria, including cellular localization, structural and evolutionary sequence similarities, taxonomic origin, carbohydrate recognition, function, and structure [26] (Figure 1). Lectins play a pivotal role in cell-cell and host-pathogen interaction and communication, tissue development, sugar storage, and other mechanisms of cell survival and immune system stimulation.…”
Section: Lectinsmentioning
confidence: 99%
“…Recombinant lectins, particularly those of plant and animal origin, are produced from microorganism expression systems by recombinant technology . Bacteria and yeasts, which have simple gene expression systems and good recombinant protein yield, are the preferred tools for recombinant lectin generation . As recombinant lectins can overcome the limitations of natural lectins, they have been increasingly used for glycan profiling .…”
Section: Factors Influencing Lectin Microarrays For Biomarker Discoverymentioning
confidence: 99%
“…Since bacteria lack organelles for posttranslational processing, yeasts are the most common system for the expression of glycoproteins, such as legume lectins. Initially, the most promising system for this purpose was Saccharomyces cerevisiae due the knowledge of its genetic manipulation [23,24]. However, S. cerevisiae has the disadvantage of producing high mannose N-glycans with outer chains of typically 50-150 residues in length (~>9 to~27 kDa), which are highly antigenic for humans [23,25].…”
Section: Introductionmentioning
confidence: 99%
“…Thus, the methylotrophic yeast Pichia pastoris, which does not appear to add extra α1-3 mannose residues or to carry out hyperglycoyslation on its N-glycans, has been successfully used as expression system for leguminous lectins [26,27]. In addition, recombinant proteins produced by Pichia can be directly recovered from the culture media by coupling them to the S. cerevisiae α-factor, which is a signal peptide that redirects the protein to the secretory pathway and is subsequently cleaved in the Golgi by Pichia endopeptidases [24].…”
Section: Introductionmentioning
confidence: 99%