Expression of Leu M1 antigen on a monoclonal B cell line established from a patient with rheumatoid arthritis

Takei, Masami; Kang, Hoil; Tomura, Kohtaro; Ikeda, Etsuko; Karasaki, Miki; Nakauchi, Hiromitsu; Okumura, Ko; Sawada, Shigemasa

doi:10.1016/0165-2478(89)90153-3

Cited by 4 publications

(5 citation statements)

References 13 publications

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“…We previously showed that TKS-1 cells express the two HL markers CD15 and CD30 [1]. Since a study has described a change in the expression of these HL markers in H-RS cells after stimulation with the tumour promoter phorbol-12- myristate-13-acetate (PMA) [24], we examined their expression in PMA-stimulated TKS-1 cells.…”

Section: Resultsmentioning

confidence: 99%

“…Peripheral blood mononuclear cells from a patient with RA were stimulated with phorbol myristate acetate (PMA) to establish the EBV + B lymphoblastoid cell line TKS [1]. Two subclones (type 1 & 2) of TKS were isolated by repeated limiting dilution in 96-well micro-culture plates.…”

Section: Methodsmentioning

confidence: 99%

“…These cells did not react against the antibodies OKM1 (CD11b), Leu M2, Leu M3 (CD14), Leu M4, or to T cell surface or natural killer cell antigens. TKS-1 cells were peroxidase-negative, slightly positive for non-specific esterase that was not inhibited by NaF, had monoclonal immunoglobulin light chain gene rearrangement and expressed EBNA-1 mRNA [1]. The RABM/EBV + 1-3 cell lines are EBV + LCLs that were established by co-cultivating bone marrow CD34 + cells obtained from RA patients with peripheral B cells isolated from an EBV-seronegative healthy individual [22].…”

Section: Methodsmentioning

confidence: 99%

“…We previously established a monoclonal EBV-infected B-lymphoblastoid cell line (LCL) called TKS-1 [1]. The disease-modifying anti-rheumatic drug (DMARD) methotrexate (MTX) sometimes causes malignancies of this type [2] and in some patients these EBV-associated lymphomas completely regress when MTX therapy is reduced or discontinued [3].…”

Section: Introductionmentioning

confidence: 99%

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Epstein-Barr-Virus-Infected CD15 (Lewis X)-Positive Hodgkin-Lymphoma-Like B Cells in Patients with Rheumatoid Arthritis

Inomata

Takei²,

Nakamura³

et al. 2009

TORJ

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Patients with rheumatoid arthritis (RA), especially those who are treated with methotrexate (MTX), might have an increased risk of Hodgkin lymphoma (HL), a malignancy that is associated with Epstein-Barr virus (EBV). Here we describe a monoclonal EBV-infected B-lymphoblastoid cell line (LCL) called TKS-1 that was established from cells that spontaneously converted from an MTX-treated RA patient. TKS-1 has properties similar to HL cells and it is distinctly different from control LCLs established from normal individuals. TKS-1 cells express the HL -associated surface markers CD15 and CD30 (Takei et al. 1989). Like Hodgkin Reed-Sternberg (H-RS) cells of EBV-positive HL, TKS-1 cells express EBNA1 mRNA transcribed from the Qp promoter of the virus, whereas control LCLs use the Cp or Wp promoter to transcribe mRNA. TKS-1 cells can proliferate in an anchorage-independent manner and possess a cloning efficiency comparable to that of the Burkitt lymphoma (BL) line Raji. In addition, two EBV-positive LCLs established by cocultivated CD34+ cells isolated from the bone marrow of patients with RA and peripheral blood B lymphocytes from a healthy EBV-seronegative individual also expressed CD15. These results indicate that EBV-infected B-lymphoblastoid cells from patients with RA tend to acquire properties similar to HL cells.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Epstein-Barr-Virus-Infected CD15 (Lewis X)-Positive Hodgkin-Lymphoma-Like B Cells in Patients with Rheumatoid Arthritis

Inomata

Takei²,

Nakamura³

et al. 2009

TORJ

Self Cite

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“…The limited distribution of CD15+ cells in the periglandial zone of the tissue samples with the same IgM+ IPCs with irregular nuclei suggests that the cells detected by immunofluorescence play a role in inflammation by recruiting CD15+ cells or by expressing CD15 themselves. However, Takei [13] found that anti-Leu M1 (anti-CD15) antibody reacted against the surface antigen of a defined monoclonal B cell line (TKS-1) in the peripheral blood of patients with rheumatoid arthritis (RA), and that these cells produced IgM in the culture supernatant and had kappa light chain rearrangements in their DNA. They were also positive for Epstein-Barr nuclear antigen.…”

Section: Discussionmentioning

confidence: 99%

Unusual B cell morphology in inflammatory bowel disease

Defendenti

Grosso

Atzeni

et al. 2012

Pathology - Research and Practice

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B lymphocytes express various different types of surface immunoglobulins that are largely unrelated to other hematological lines, although some reports have described a relationship between malignant B cells and other cells such as macrophages. Multiple genes of hematopoietic lineage, including transcription factors, are co-expressed in hematopoietic stem cells and progenitors, a phenomenon referred to as "lineage priming". Changes in the expression levels and timing of transcription factors can induce the lineage conversion of committed cells, which indicates that the regulation of transcription factors might be particularly critical for maintaining hierarchical hematopoietic development. The aim of this study was to evaluate the surface markers of particular IgM-positive and irregularly nucleated cells detected in patients with inflammatory bowel disease (IBD), and to assess their association with diagnosis and inflammatory cell recruitment. Small intestine, colon and rectal biopsy specimens of 96 IBD patients were studied. Immunoglobulin-producing cells (IPCs) were analyzed by means of immunofluorescence using polyclonal rabbit anti-human Ig and goat anti-human IgM. The specimens positive for B cells with irregular nuclei were assessed using monoclonal antibodies specific for CD79, and λ and κ chains in order to confirm their B cell nature. CD15+ cells, an important marker of inflammatory cell recruitment, were also evaluated. Statistical correlations were sought between the histological findings and clinical expression. 34 (35.4%) of the 96 patients (64 with ulcerative colitis and 32 with Crohn's disease) presented a periglandial localization of IPCs with irregular nuclei, which showed surface markers specific for the B cell subset, such as IgM and CD79, but quantitative differences in λ and κ chains. These specimens also contained CD15-positive cells, which are usually absent in healthy controls. The quantitative aspects and localization of the CD15-positive cells correlated with the distribution of the IPCs with irregular nuclei. IPCs with irregular nuclei were significantly more frequent in those patients with Crohn's disease than in those with ulcerative colitis (p<0.001). The finding of a subpopulation of cells that simultaneously showed irregular nuclei and B cell markers, such as functional surface IgM, in patients with IBD suggests that an unusual subgroup of B cells that correlates with CD15 expression and a diagnosis of Crohn's disease may be observed in the inflammatory process.

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Targeting TNF superfamily members for therapeutic intervention in rheumatoid arthritis

Vinay

Kwon

2012

Cytokine

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Expression of Leu M1 antigen on a monoclonal B cell line established from a patient with rheumatoid arthritis

Cited by 4 publications

References 13 publications

Epstein-Barr-Virus-Infected CD15 (Lewis X)-Positive Hodgkin-Lymphoma-Like B Cells in Patients with Rheumatoid Arthritis

Epstein-Barr-Virus-Infected CD15 (Lewis X)-Positive Hodgkin-Lymphoma-Like B Cells in Patients with Rheumatoid Arthritis

Unusual B cell morphology in inflammatory bowel disease

Targeting TNF superfamily members for therapeutic intervention in rheumatoid arthritis

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