Expression of leukocyte adhesion molecules by endothelial cells seeded on various polymer surfaces

Imbert, E.; Poot, Andreas A.; Figdor, Carl G.; Feijén, Jan

doi:10.1002/1097-4636(20010905)56:3<376::aid-jbm1106>3.0.co;2-r

Cited by 20 publications

(1 citation statement)

References 24 publications

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“…HUVEC, analyzed by flow cytometry after removal from TCPS, Dacron, and Teflon showed no E-selectin and low levels of VCAM-1, independent of substrate, and HOTMEC showed no VCAM-1 and low levels of E-selectin. PECAM and ICAM-1 levels were similar in both cell types [145]. The process required to remove the cells from the initial culture substrate however complicates drawing conclusions from this flow cytometry study.…”

Section: Molecular Expression Studiesmentioning

confidence: 88%

The influence of biomaterials on endothelial cell thrombogenicity

2007

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Driven by tissue engineering and regenerative medicine, endothelial cells are being used in combination with biomaterials in a number of applications for the purpose of improving blood compatibility and host integration. Endothelialized vascular grafts are beginning to be used clinically with some success in some centers, while endothelial seeding is being explored as a means of creating a vasculature within engineered tissues. The underlying assumption of this strategy is that when cultured on artificial biomaterials, a confluent layer of endothelial cells maintain their nonthrombogenic phenotype. In this review the existing knowledge base of endothelial cell thrombogenicity cultured on a number of different biomaterials is summarized. The importance of selecting appropriate endpoint measures that are most reflective of overall surface thrombogenicity is the focus of this review. Endothelial cells inhibit thrombosis through three interconnected regulatory systems (1) the coagulation cascade (2) the cellular components of the blood such as leukocytes and platelets and (3) the complement cascade, and also through effects on fibrinolysis and vascular tone, the latter which influences blood flow. Thus, in order to demonstrate the thromobgenic benefit of seeding a biomaterial with EC, the conditions under which EC surfaces are more likely to exhibit lower thrombogenicity than unseeded biomaterial surfaces need to be consistent with the experimental context. The endpoints selected should be appropriate for the dominant thrombotic process that occurs under the given experimental conditions.

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Section: Molecular Expression Studiesmentioning

confidence: 88%

The influence of biomaterials on endothelial cell thrombogenicity

2007

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Novel Brush Polymers with Phosphorylcholine Bristle Ends: Synthesis, Structure, Properties, and Biocompatibility

Kim

Park

Jung

et al. 2009

Adv Funct Materials

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New brush polymers with various numbers of bristle ends incorporating phosphorylcholine (PC) moieties are synthesized. The polymers are thermally stable up to 175 °C and form good‐quality films with conventional spin‐, roll‐, and dip‐coating, and subsequent drying processes. Interestingly, all these brush polymers, as a PC‐containing polymer, demonstrate a stable molecular multi‐bilayer structure in thin films that arise due to the efficient self‐assembly of the bristles for temperatures <55 °C and PC‐rich surfaces, and therefore successfully mimic natural cell‐membrane surfaces. These brush‐polymer films exhibit excellent water wettability and water sorption whilst retaining the remarkable molecular multi‐bilayer structure, and thus have hydrophilic surfaces. These novel multi‐bilayer structured films repel fibrinogen molecules and platelets from their surfaces but also have bactericidal effects on bacteria. Moreover, the brush‐polymer films are found to provide comfortable surface environments for the successful anchoring and growth of HEp‐2 cells, and to exhibit excellent biocompatibility in mice. These newly developed brush polymers are suitable for use in biomedical applications including medical devices and biosensors that require biocompatibility and the reduced possibility of post‐operative infection.

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ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Zijpp

Poot

Feijén

2003

J Biomedical Materials Res

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Small-diameter vascular grafts rapidly fail as a result of blood coagulation and platelet deposition. Endothelial cells lining the inner side of blood vessels can provide the graft lumen with an antithrombogenic surface. One of the remaining problems is cell detachment after restoration of blood flow, because of infiltration of leukocytes that respond to an inflammatory-like activation of the endothelial cells. This endothelial activation is possibly caused by the surface characteristics of the underlying polymer. To get more insight into the effects of the polymer surface on endothelial cell activation, we seeded human umbilical vein endothelial cells (HUVECs) in various densities and subsequently grew them on tissue culture polystyrene (TCPS; hydrophilic) and polystyrene (PS; hydrophobic) surfaces. To improve cell adhesion, surfaces were coated with purified fibronectin prior to cell seeding. During proliferation, the expressions of the leukocyte adhesion molecules ICAM-1 and VCAM-1 were determined. Results indicate that ICAM-1 expression is not influenced by the character of the polymer surface, and that VCAM-1 expression is slightly higher on the TCPS surface. Expressions of both adhesion molecules are influenced by the seeding density and time of proliferation. At low seeding densities (< or = 10,000 cells/cm(2)), a relatively low percentage of nonexogenously activated cells expressed ICAM-1 during the first 3 days of proliferation compared to higher seeding densities. Although less pronounced, this was also observed for the percentage of cells expressing VCAM-1. During proliferation, the amount of ICAM-1 per endothelial cell increased, whereas the expression of VCAM-1 remained low. The absence of large differences in leukocyte adhesion molecule expression by endothelial cells grown on TCPS or PS is possibly caused by coating of the surfaces with fibronectin. It is known that surface hydrophilicity influences protein adsorption. Although this had no or little effect on leukocyte adhesion molecule expression, endothelial cell growth was affected, because proliferation was slower on the hydrophobic PS.

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Expression of leukocyte adhesion molecules by endothelial cells seeded on various polymer surfaces

Cited by 20 publications

References 24 publications

The influence of biomaterials on endothelial cell thrombogenicity

The influence of biomaterials on endothelial cell thrombogenicity

Novel Brush Polymers with Phosphorylcholine Bristle Ends: Synthesis, Structure, Properties, and Biocompatibility

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

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