Expression of Light-Harvesting Chlorophyll <i>a/b</i>-Protein Genes Is Phytochrome-Regulated in Etiolated <i>Arabidopsis thaliana</i> Seedlings

Karlin-Neumann, George; Sun, Lin; Tobin, Elaine M.

doi:10.1104/pp.88.4.1323

Cited by 117 publications

(83 citation statements)

References 37 publications

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“…the levels of GapA and GapB mRNA reverted to their basal levels in the dark. Similar results have been observed for Cab genes of Arabidopsis, tobacco, and tomato (Karlin-Neumann et al, 1988;Wehmeyer et al, 1990). Our results further indicate that the levels of induction by short light pulses are much lower than the difference in mRNA levels seen between seedlings germinated under continuous white light and complete darkness.…”

Section: Discussionsupporting

confidence: 74%

“…5, panel Cab, lanes 1 and 2), and this increase could be partially reversed by far-red light treatment (lane 3). These results are consistent with those published previously by other laboratories (Karlin-Neumann et al, 1988;Chory et al, 1989). For a.…”

Section: Effects Of Light Pulses On Gap Gene Expression In Etiolated supporting

confidence: 83%

“…these increases revert to basal levels after 24 h in the dark (data not shown). Similar results have been observed for the Cab genes of Arabidopsis, tobacco, and tomato (Karlin-Neumann et al, 1988;Wehmeyer et al, 1990). These results suggest that the continuous presence of light may be required for sustained and maximal expression of these genes in etiolated seedlings.…”

Section: Effects Of Light Pulses On Gap Gene Expression In Etiolated supporting

confidence: 73%

“…The blue light source was a 150-W Sylvania halogen flood light filtered through a Ditric Optics 430-nm bandpass filter (bandwidth 8 nm); the light intensity at the leaf surface was 1 pE m-'s-I. The intensities for the red and far-red light treatments were the same as those described by Karlin-Neumann et al (1988) for studying Cab genes. Cooling was achieved by circulating chilled water through glass trayis between the light source and the bandpass filters.…”

Section: Light Sourcesmentioning

confidence: 99%

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Effects of Blue and Red Light on Expression of Nuclear Genes Encoding Chloroplast Glyceraldehyde-3-Phosphate Dehydrogenase of Arabidopsis thaliana

Dewdney¹,

Conley²,

Shih³

et al. 1993

Plant Physiol.

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We have characterized the effects of different light spedra on expression of the nuclear genes (GapA and GapS) encoding chloroplast glyceraldehyde-3-phosphate dehydrogenase in Arabidopsis fhaliana. Steady-state mRNA levels for both genes in etiolated seedlings increased after a short exposure to red or blue light. However, these increases could not be reversed by immediate farred light following the initial light treatment. In mature plants, a short light pulse, regardless of its spedrum, had no apparent effect on GapA or GapS mRNA levels in dark-adapted plants. In contrast, continuous exposure to red, blue, or white light resulted in increases of GapA and GapS mRNA levels, with blue and white light being far more efficient than red light. Similarly, continuous exposure of etiolated seedlings to red, blue, or white light also resulted in increased CapA and CapB mRNA levels. In addition, we show that illumination of red light-saturated Arabidopsis plants with continuous blue light results in further increases of GapA and GapS mRNA levels. Based on these results, we conclude that both blue light photoreceptor-and phytochrome-mediated pathways are involved in light regulation of GapA and GapS genes in Arabidopsis, with blue light acting as the dominant regulator.

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Section: Discussionsupporting

confidence: 74%

Section: Effects Of Light Pulses On Gap Gene Expression In Etiolated supporting

confidence: 83%

Section: Effects Of Light Pulses On Gap Gene Expression In Etiolated supporting

confidence: 73%

Section: Light Sourcesmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Blue and Red Light on Expression of Nuclear Genes Encoding Chloroplast Glyceraldehyde-3-Phosphate Dehydrogenase of Arabidopsis thaliana

Dewdney¹,

Conley²,

Shih³

et al. 1993

Plant Physiol.

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“…The light pulse treatments can induce gene expression, without significantly altering the development or physiology of the etiolated seedling. This induction is mediated at least in part by the red/far-red light receptor phytochrome (Karlin-Neumann et al, 1988;) and a blue light receptor (Gao and Kaufman, 1994). We wanted to know which photoreceptor(s), if any, might be involved in the reduced CA6 expression in continuous white light in cuel.…”

Section: Blue and White Lightmentioning

confidence: 99%

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis.

et al. 1995

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Light plays a key role in the development and physiology of plants. One of the most profound effects of light on plant development is the derepression of expression of an array of light-responsive genes, including the genes encoding the chlorophyll alb binding proteins (CAB) of photosystem II. To understand the mechanism by which light signals nuclear gene expression, we developed a genetic selection to identify mutants with reduced CAB transcription. Here, we describe a new Arabidopsis locus, COE7 (for CAS ynderexpressed). Mutations at this locus result in defects in expression of several light-regulated genes, specifically in mesophyll but not in bundle-associated or epidermis cells. Reduced accumulation of CAB and other photosynthesis-related mRNAs in the mesophyll was correlated with defects in chloroplast development in these cells, resulting in a reticulate pattern with veins greener than the interveinal regions of leaves.Moreover, chalcone synthase mRNA, although known to be regulated by both phytochrome and a blue light receptor, accumulated normally in the leaf epidermis. Dark basal levels of CAB expression were unaffected in etiolated cueí seedlings; however, induction of CAB transcription by pulses of red and blue light was reduced, suggesting that CUEl acts downstream from both phytochrome and blue light photoreceptors. CUEl appears to play a role in the primary derepression of mesophyll-specific gene expression in response to light, because cueí mutants are severely deficient at establishing photoautotrophic growth. Based on this characterization, we propose that CUEí is a cell-specific positive regulator linking light and intrinsic developmental programs in Arabidopsis leaf mesophyll cells.

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Expression of a monocot LHCP promoter in transgenic rice.

et al. 1991

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The beta‐glucuronidase (GUS) gene linked to the promoter of rice light harvesting chlorophyll a/b‐binding protein of photosystem II (LHCPII) was introduced into rice by electroporation. Expression of the GUS gene with the LHCP promoter in transformed protoplasts and callus was much lower than with the cauliflower mosaic virus (CaMV) 35S promoter. In green organs, however, LHCP‐GUS activity with the LHCP promoter was 10‐fold higher than that with the CaMV 35S promoter. Expression of the LHCP‐GUS gene was detected in leaves, stems and floral organs but not in roots. Histochemical analysis of leaves showed that the GUS gene was expressed specifically in green tissues. In selfed seeds of the transformants, GUS gene expression was detectable only in the germinated embryos after greening. Northern blot analysis revealed that transcription of the GUS gene with the LHCP promoter was not detectable in dark‐grown seedlings of transformants but was induced by exposure to light. These results show that regulation of the introduced chimeric gene in transgenic plants was characteristic of the LHCP gene itself.

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Expression of Light-Harvesting Chlorophyll a/b-Protein Genes Is Phytochrome-Regulated in Etiolated Arabidopsis thaliana Seedlings

Cited by 117 publications

References 37 publications

Effects of Blue and Red Light on Expression of Nuclear Genes Encoding Chloroplast Glyceraldehyde-3-Phosphate Dehydrogenase of Arabidopsis thaliana

Effects of Blue and Red Light on Expression of Nuclear Genes Encoding Chloroplast Glyceraldehyde-3-Phosphate Dehydrogenase of Arabidopsis thaliana

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis.

Expression of a monocot LHCP promoter in transgenic rice.

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