Expression of Lipid Peroxidation Markers in the Tear Film and Ocular Surface of Patients with Non-Sjogren Syndrome: Potential Biomarkers for Dry Eye Disease

Choi, Won Suk; Chen, Lian; Li, Ying; Kim, Ga-Eon; You, In Cheon; Park, Soo Hyun; Yoon, Kyung Chul

doi:10.3109/02713683.2015.1098707

Cited by 81 publications

(79 citation statements)

References 37 publications

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“…19 A limited number of publications investigating biomarkers in human tears for other steps in the lipid peroxidation pathway are available; increased expression was reported for two later stage products, 4-hydroxy-2-nonenal and malondialdehyde, in those with dry eye and increased age. 20,21 As HEL represents only one step in the lipid peroxidation pathway, future additional studies evaluating tears and ocular surface samples for different types of lipid peroxidation products would help clarify the potential significance of other lipid peroxidation effects.…”

Section: Discussionmentioning

confidence: 99%

“…14,15,20,22,23 Reasons for this could be experimental differences in tear sample preparation and analysis, environmental-induced variation (i.e., previous reports are all from Japan), dietary differences, or genetic variation in HEL production. In support of diet-induced variation, it is well established that consumption of omega-6 fatty acids is much higher for those who choose a Western diet compared to those who consume a traditional Japanese diet.…”

Section: Discussionmentioning

confidence: 99%

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Oxidative Stress Measures of Lipid and DNA Damage in Human Tears

Haworth

Chandler

2017

Invest. Ophthalmol. Vis. Sci.

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PurposeWe evaluate feasibility and repeatability of measures for lipid peroxidation and DNA oxidation in human tears, as well as relationships between outcome variables, and compared our findings to previously reported methods of evaluation for ocular sun exposure.MethodsA total of 50 volunteers were seen for 2 visits 14 ± 2 days apart. Tear samples were collected from the inferior tear meniscus using a glass microcapillary tube. Oxidative stress biomarkers were quantified using enzyme-linked immunosorbent assay (ELISA): lipid peroxidation by measurement of hexanoyl-lysine (HEL) expression; DNA oxidation by measurement of 8-oxo-2′-deoxyguinosone (8OHdG) expression. Descriptive statistics were generated. Repeatability estimates were made using Bland-Altman plots with mean differences and 95% limits of agreement were calculated. Linear regression was conducted to evaluate relationships between measures.ResultsMean (±SD) values for tear HEL and 8OHdG expression were 17368.02 (±9878.42) nmol/L and 66.13 (±19.99) ng/mL, respectively. Repeatability was found to be acceptable for both HEL and 8OHdG expression. Univariate linear regression supported tear 8OHdG expression and spring season of collection to be predictors of higher tear HEL expression; tear HEL expression was confirmed as a predictor of higher tear 8OHdG expression.ConclusionsWe demonstrate feasibility and repeatability of estimating previously unreported tear 8OHdG expression. Seasonal temperature variation and other factors may influence tear lipid peroxidation. Support is demonstrated to suggest lipid damage and DNA damage occur concurrently on the human ocular surface.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Oxidative Stress Measures of Lipid and DNA Damage in Human Tears

Haworth

Chandler

2017

Invest. Ophthalmol. Vis. Sci.

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show abstract

Section: Discussionmentioning

confidence: 99%

“…Considering that most reactive oxygen species (ROS) are highly reactive and short‐lived, it is difficult to detect them in biological substances . Therefore, measurement of the end products of lipid peroxidation is one of the most widely accepted approaches for evaluating oxidative damage . 4‐HNE, which is commonly used as a marker of lipid peroxidation, as well as oxidative DNA and/or protein damage, is the major 4‐hydroxyalkenal end product generated by the decomposition of polyunsaturated fatty acids .…”

Section: Discussionmentioning

confidence: 99%

Evaluations of lipid peroxidation and inflammation in short‐term glycerol‐induced acute kidney injury in rats

Nara

Yajima

Abe

et al. 2016

Clin Exp Pharma Physio

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Rhabdomyolysis is characterised by acute kidney injury (AKI) resulting from skeletal muscle injury. Lipid peroxidation-mediated oxidant injury and pro-inflammatory cytokine-mediated inflammatory response play critical roles in the pathogenesis of rhabdomyolysis-induced AKI. The present study aimed to investigate the short-term effects of both lipid peroxidation and inflammatory responses on rhabdomyolysis-induced AKI in a rat model of glycerol-induced rhabdomyolysis. Rhabdomyolysis was induced by the intramuscular injection of 50% glycerol in saline (10 mL/kg) into the hind limbs of rats. Rats were killed 1 or 3 hours after glycerol injection. Time-dependent increases in serum biochemical parameters, including blood urea nitrogen, creatinine, lactate dehydrogenase and creatine phosphokinase levels, were observed 1 hour after glycerol injection. In kidneys, glycerol injection resulted in histopathological changes such as renal tubular injury and renal tubular myoglobin deposition. Levels of Nε-(hexanoyl)lysine-modified, 4-hydroxy-2-nonenal-modified, and nitrotyrosine-modified proteins in rat kidneys were unaltered at 1 hour after glycerol injection, but increased significantly at 3 hours. Increases in renal nitric oxide production and the expression levels of inducible nitric oxide synthase, interleukin-6 and tumour necrosis factor-α in the renal parenchyma were observed at 1 hour after glycerol injection and plateaued at 3 hours. Our findings suggest that the pro-inflammatory cytokine-mediated inflammatory response may cause rhabdomyolysis-induced AKI very shortly after glycerol injection, and lipid peroxidation-mediated oxidant injury may promote the development of these pathophysiological processes.

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“…40,57,58 In vivo lipid oxidative injury has been observed in DED and Sjögren's syndrome patients. 59,60 In vitro studies further revealed that lipid and mitochondrial oxidative damage causes inflammation in HCE cells. 39,61 As mentioned above, various anti-inflammatory drugs, such as topical steroids and cyclosporine, have been widely used to treat DED, and studies have shown that anti-inflammatory treatment has a beneficial effect on tear secretion, tear film stability, and ocular surface integrity.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Lee

Choi

Cui

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Citation: Lee HS, Choi J-H, Cui L, et al. Anti-inflammatory and antioxidative effects of Camellia japonica on human corneal epithelial cells and experimental dry eye: in vivo and in vitro study. Invest Ophthalmol Vis Sci. 2017;58:119658: -120758: . DOI:10.1167 PURPOSE. To analyze the anti-inflammatory and antioxidative effects of Camellia japonica (CJ) on human corneal epithelial (HCE) cells and its therapeutic effects in a mouse model of experimental dry eye (EDE). METHODS.Camellia japonica extracts of varying concentrations (0.001%, 0.01%, and 0.1%) were used to treat HCE cells. Dichlorofluorescein diacetate (DCF-DA) and dihydroethidium (DHE) assays were performed. The production of peroxiredoxin (PRX) 1-6 and manganesedependent superoxide dismutase (MnSOD) in HCE cells was assessed using Western blot analysis. Furthermore, eye drops containing 0.001%, 0.01%, or 0.1% CJ extract or a balanced salt solution (BSS) were applied to the EDE. Clinical parameters were measured 7 days after treatment. The levels of inflammatory markers and intracellular reactive oxygen species (ROS) were measured.RESULTS. Treatment with 0.01% and 0.1% CJ extracts decreased apoptosis in HCE cells. In addition, band intensities of PRX 1, 4, and 5, as well as MnSOD, after hydrogen peroxide (H 2 O 2 ) treatment showed a significant improvement after pretreatment with 0.01% and 0.1% CJ extracts. Mice treated with 0.1% CJ extract showed significantly improved clinical parameters when compared to those of the EDE control and BSS groups. A significant decrease in the levels of inflammatory markers and intracellular ROS was observed in the 0.01% and 0.1% CJ extract groups.CONCLUSIONS. Camellia japonica extracts promoted antioxidative protein expression and suppressed apoptosis in HCE cells. Furthermore, CJ extracts improved clinical signs of dry eye and reduced oxidative stress and the expression of inflammatory markers, suggesting that eye drops containing CJ extract could be used as an adjunctive treatment for dry eye.

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Expression of Lipid Peroxidation Markers in the Tear Film and Ocular Surface of Patients with Non-Sjogren Syndrome: Potential Biomarkers for Dry Eye Disease

Abstract: The expression of late lipid peroxidation markers, 4-HNE and MDA, increases in the tear film and ocular surface of patients with dry eye. The levels correlate with various tear film and ocular surface parameters and may reflect the severity of dry eye disease.

Cited by 81 publications

References 37 publications

Oxidative Stress Measures of Lipid and DNA Damage in Human Tears

Oxidative Stress Measures of Lipid and DNA Damage in Human Tears

Evaluations of lipid peroxidation and inflammation in short‐term glycerol‐induced acute kidney injury in rats

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

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