Expression of lncRNAs NEAT1 and lnc-DC in Serum From Patients With Behçet’s Disease Can Be Used as Predictors of Disease

Abstract

Background: Behçet’s disease (BD) is a chronic autoimmune disease. The early diagnosis of BD is very important to avoid serious and/or fatal complications such as eye damage, severe neurological involvement, and large vessel occlusion. New, sensitive biomarkers would aid in rapid diagnosis, the monitoring of disease activity, and the response to treatment.Methods: This study’s aim is to identify two immune system-related BD biomarkers. We measured long non-coding RNAs (lncRNAs) NEAT1 (nuclear-enriched abundant transcript 1), and lnc-DC (lncRNA in dendritic cells) in serum by real-time polymerase chain reaction (RT-PCR) in 52 BD patients and 52 controls. We analyzed the association between NEAT1 and lnc-DC and the clinical parameters of BD. Receiver operating characteristic (ROC) curve analysis was performed to explore the diagnostic performance of the studied genes.Results: Compared to controls, the significant upregulation of NEAT1 {median [interquartile range (IQR)] = 1.68 (0.38–7.7), p < 0.0001} and downregulation of lnc-DC [median (IQR) = 0.2 (0.12–1.39), p = 0.03] were detected in the sera collected from BD patients. Higher serum expression levels of NEAT1 and lnc-DC were significantly associated with the following clinical presentations: cutaneous lesions, vascular manifestations, articular manifestations, neurological manifestations, and higher disease activity score. Also, high NEAT1 levels were significantly associated with a negative pathergy test, while higher lnc-DC was significantly associated with a positive family history. ROC curves showed that NEAT1 and lnc-DC levels in serum could be used as predictors of BD with high specificity and fair sensitivity. NEAT1 had an area under the curve (AUC) of 0.692 (95% CI: 0.591–0.794, p = 0.001), and lnc-DC had an AUC of 0.615 (95% CI: 0.508–0.723, p = 0.043).Conclusion: Serum lncRNAs NEAT1 and lnc-DC are biomarkers for BD.

Association of long non-coding RNAs NEAT1, and MALAT1 expression and pathogenesis of Behçet's disease among Egyptian patients

“…NEAT1 may be used to monitor and control subclinical inflammatory reactions of BD, thus, preventing the diseases progression and development. On the other hand, our results may disagree with the outcome of the study by Mohammed and coworkers ( Mohammed et al, 2021 ). This could be attributed to the difference in the applied therapeutic options as well as disease state, patient’s symptoms, and subgroups.…”

Non-coding RNA NEAT-1 and interleukin-6 as diagnostic indicators for vitiligo

“…To the best of our knowledge, no previous studies illustrated the role of NEAT-1 in vitiligo patients. However, studies agreed with our results that the expression of NEAT-1 tends to increase in autoimmune diseases such as RA, SLE, multiple sclerosis and Behçet's disease which are autoimmune diseases like vitiligo [7,8,19,20].…”

The Ifng antisense RNA 1 (IFNG-AS1) and growth arrest-specific transcript 5 (GAS5) are novel diagnostic and prognostic markers involved in childhood ITP

“…It has previously been shown that lncRNAs were expressed in serum ( Mohammed et al, 2022 )– ( Visconti et al, 2020 ). The RT2 SYBR Green PCR kit (Qiagen, Maryland, USA) was used to evaluate the expression of the lncRNAs IFNG-AS1 and GAS5 in serum using specific primers supplied by Qiagen, (Valencia, CA, USA); for IFNG-AS1 (Catalog no; 330701LPH20079A, Accession no, ENST00000536914.0) and GAS5 (Catalog no; 330701LPH11340A, Accession no, NR_002578.2) and we used GAPDH primer (Catalog no: 330701 LPH31725A, Accession no: ENST00000496049.0) to standardize the expression pattern and quantify the target long non-coding RNAs ( Li et al, 2016 ), ( Peng et al, 2021 ).…”