Expression of low molecular weight HSP 70 related polypeptides from the symbiotic sea anemone Anemonia viridis forskall in response to heat shock

Sharp, Vincent A.; Miller, David J.; Bythell, John C.; Brown, Barbara E.

doi:10.1016/0022-0981(94)90113-9

Cited by 68 publications

(33 citation statements)

References 28 publications

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“…HSP 70 and 90 expression has been previously reported in cnidarians by others using the same or similar techniques (Miller et al 1992;Sharp et al 1994;Black et al 1995;Fang et al 1997;Sharp et al 1997;Wiens et al 2000;Rossi and Snyder 2001;Snyder and Rossi 2004).…”

Section: Hsp 70 and Hsp 90 Measurementsmentioning

confidence: 53%

Protein, carbohydrate, lipid concentrations and HSP 70–HSP 90 (stress protein) expression over an annual cycle: useful tools to detect feeding constraints in a benthic suspension feeder

2005

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Protein, carbohydrate, lipid concentrations and HSP 70-HSP 90 (stress protein) expression over an annual cycle: useful tools to detect feeding constraints in a benthic suspension feeder Abstract In the present paper we suggest an effect of seasonal variations in food availability on two ecophysiological parameters in a warm temperate benthic suspension feeder: the tissue concentrations of proteins, carbohydrates and lipids on the one hand, and the expression of stress proteins (HSP 70 and 90, inducible and/or constitutive) on the other hand. The concentrations of biomacromolecules have already been used to describe bentho-pelagic and reproductive processes, but this is the first time that stress protein expression is suggested to be directly related with food constraints in marine organisms. Paramuricea clavata (Cnidaria: Gorgonacea) express HSP 70 and 90 (constitutive and/ or inducible) throughout the seasonal cycle, and HSP 70 levels are twice as high as the levels of HSP 90. In summer and autumn, when seston availability to suspension feeders was low, P. clavata showed low levels of carbohydrates and lipids, but high levels of HSPs expression. The levels of HSP 70 and 90 expression fit with negative exponential functions of carbohydrate and lipid concentrations. We suggest a direct effect of food availability on the studied ecophysiological parameters while the effect of temperature may be rather indirect. HSP expression as well as the tissue concentrations of carbohydrate and lipids may be used as biomarkers of environmental changes and seston availability to benthic suspension feeders.

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Section: Hsp 70 and Hsp 90 Measurementsmentioning

confidence: 53%

Protein, carbohydrate, lipid concentrations and HSP 70–HSP 90 (stress protein) expression over an annual cycle: useful tools to detect feeding constraints in a benthic suspension feeder

2005

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“…Experimental evidence that HSPs are involved in conferring tolerance to environmental extremes, such as elevated temperature, is abundant in both aquatic and nonaquatic species including Anthozoans (Sanders, 1993;Sharp et al, 1994Sharp et al, , 1997Black et al, 1995;Hayes and King, 1995;Fang et al, 1997;Tom et al, 1999). Many studies have demonstrated HSP70 and low molecular weight HSPs as the major HSPs induced as a result of raised temperature.…”

Section: Discussionmentioning

confidence: 99%

“…In certain marine habitats, sessile lower invertebrates like sea anemones are directly exposed to extreme environmental conditions, such as elevated temperatures, which may cause tissue damage (Sharp et al, 1994). Stress proteins are expected to play a significant role in conferring tolerance to such harsh conditions upon these organisms.…”

Section: Introductionmentioning

confidence: 99%

The 60-kDa Heat Shock Protein (HSP60) of the Sea Anemone Anemonia viridis: A Potential Early Warning System for Environmental Changes

Choresh

Ron

Loya

2001

Marine Biotechnology

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Expression of heat shock proteins (HSPs) is often correlated with adaptation to environmental stress.We examined the role of HSP60 (60 kDa) in acclimatization to thermal stress in the sea anemone Anemonia viridis. Using monoclonal antibodies, we identified HSP60 in sea anemones for the first time, and showed that its expression varied with changes in seawater temperature (SWT). Anemonia viridis displayed high levels of HSP60 when extreme temperatures prevailed in stressful habitats such as tidal pools. Specimens sampled from different temperature layers in the same tidal pool differed in their levels of HSP60. Specimens from subtidal zones exhibited a seasonal pattern of expression of HSP60, according to the seasonal SWT. The level of HSP60 was significantly higher in the summer (SWT, 31°C) than in other seasons throughout the year. This study suggests the use of HSP60 expression as a tool for stress detection in marine invertebrates.

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“…Stress protein responses have been examined in other cnidarians (Bosch and Praetzel, 1991;Miller et al, 1992;Sharp et al, 1994Sharp et al, , 1997Black et al, 1995;Fang et al, 1997;Brennecke et al, 1998;Wiens et al, 2000), but, to our knowledge, direct measurements of HSP protein responses have not been performed on intact cnidarians in situ during natural stress cycles such as tidal fluctuations. All studies have involved laboratory acclimated organisms or organisms brought to the laboratory and sampled under artificial conditions.…”

Section: Discussionmentioning

confidence: 99%

Stress protein (HSP70 family) expression in intertidal benthic organisms: the example of <i>Anthopleura elegantissima</i> (Cnidaria: Anthozoa)

Snyder¹,

Rossi²

2004

Sci. Mar.

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Both the physiology and distribution of intertidal organisms are strongly influenced by different kinds of physical stress. Temperature, UV radiation and desiccation in low tide conditions are usually considered the most important physical stresses. These factors impact cell metabolism, and the organism's ability to rapidly adapt to altering environmental conditions sets its tidal distribution limits. The role of the HSP70 protein (stress protein) family in thermal stress responses has been widely demonstrated. Although it has been shown that stress protein expression is a useful tool to quantify part of this physical stress, few studies have been made with different in situ intertidal organisms. To test differences in HSP70 expression under natural conditions in the intertidal environment, we chose a common cnidarian of the eastern Pacific Ocean: the anemone Anthopleura elegantissima. Polyp HSP70 expression depends on the degree of emersion and the extent of physical stress (0.1-3.6 ± 1.5 ng HSP70 µg Protein -1 were registered between fully immersed and fully emersed polyps of the same clone). The time of immersion is reflected in the recovery of polyp HSP70 levels from the high tidal exposure (reaching again as shallower clones express more HSP70 (2.5 ng HSP70 µg P -1 ), than deeper ones (0.1 ng HSP70 µg P -1 ). Also sunny and foggy environmental situations influence the stress response. Anemone clones exposed to the sunny high intertidal zone express more than three times HSP70 (2.2 ng HSP70 µg P -1 ) than those on a foggy high intertidal day (0.6 ng HSP70 µg P -1 ). For A. elegantissima, shrinking of polyps, mucus secretion, sand covering, UV absorbing molecules, and a tight patch structure work concurrently with HSP70 expression to alleviate the effects of physical stress in low tidal emersion.Key words: Anthopleura, stress proteins, HSPs, intertidal stress, temperature RESUMEN: EXPRESIÓN DE LAS PROTEÍNAS DE ESTRÉS (FAMILIA DE LA HSP70) EN ORGANISMOS BENTÓNICOS INTERMAREALES:EL EJEMPLO DE ANTHOPLEURA ELEGANTISSIMA (CNIDARIOS: ANTOZOOS). -Tanto la fisiología como la distribución de los organismos intermareales están muy influenciados por distintos tipos de estrés físico. Temperatura, radiación ultravioleta y desecación en condiciones de bajamar se suelen considerar los estreses físicos más importantes. Dichos factores ejercen su impacto sobre el metabolismo celular, y la capacidad del organismo de adaptarse rápidamente a condiciones ambientales alteradas establece sus límites de distribución mareal. Se ha demostrado de forma general el papel de la familia de proteí-nas HSP70 (proteínas de estrés) en las respuestas al estrés térmico. Aunque se ha demostrado que la expresión de la proteína de estrés es una herramienta útil para cuantificar parte de este estrés físico, se han realizado pocos estudios con diferentes organismos intermareales in situ. Para comprobar diferencias en la expresión de la HSP70 bajo condiciones naturales en el ambiente intermareal, elegimos un cnidario común del océano Pacífic...

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Expression of low molecular weight HSP 70 related polypeptides from the symbiotic sea anemone Anemonia viridis forskall in response to heat shock

Cited by 68 publications

References 28 publications

Protein, carbohydrate, lipid concentrations and HSP 70–HSP 90 (stress protein) expression over an annual cycle: useful tools to detect feeding constraints in a benthic suspension feeder

Protein, carbohydrate, lipid concentrations and HSP 70–HSP 90 (stress protein) expression over an annual cycle: useful tools to detect feeding constraints in a benthic suspension feeder

The 60-kDa Heat Shock Protein (HSP60) of the Sea Anemone Anemonia viridis: A Potential Early Warning System for Environmental Changes

Stress protein (HSP70 family) expression in intertidal benthic organisms: the example of <i>Anthopleura elegantissima</i> (Cnidaria: Anthozoa)

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