Expression of Ly 1, Ly 2, Thy 1, and TL differentiation antigens on mouse T-cell tumors.

Abstract

Transplanted lymphomas, most of thymic origin, induced in BALB/c mice with 1-ethyl-1-nitrosourea (ENU) and transplanted spontaneously occurring lymphomas of AKR mice were examined for the expression of the T-cell antigens Ly, TL, and Thy 1 by using three serological methods. Most (11 of 13) of the Thy 1+ and/or TL+ tumors, i.e., T-cell tumors, expressed high levels of either Ly 1 or Ly 2 antigen, but not both. Thus most thymic lymphocytic tumors expressed restricted Ly phenotypes comparable to phenotypes previously described for functional peripheral T cells. Because tumor phenotypes were stable over a number of transplant generations, they therefore appeared to be an intrinsic property of the specific tumors. The majority of the BALB/c lymphomas were Ly 1- 2+ and also positive with anti-TL antiserum. This predominant phenotype on the BALB/c tumors may be related to either the mode of tumor induction or to the mouse strain, but since the restricted Ly pattern was observed both in BALB/c and AKR tumors, the phenotypic restriction itself is not a consequence of either of these factors. Tumor induction by ENU per se is not responsible for Ly or TL ,ntigen expression since several non-T-cell BALB/ c tumors, also induced by ENU, did not express either Ly or TL antigens. Data presented here suggest that the target cell for leukemogenesis may be a partially differentiated thymus cell. The restricted expression of Ly antigens on differentiating thymus cells to either the (formula: see text), phenotype may occur before the loss of TL antigen.

Chromosome 15 trisomy in spontaneous and carcinogen‐induced murine lymphomas of B‐cell origin

“…Group 3 consisted of five carcinogen-induced leukemias. Four tumors were induced by DMBA in different F, hybrid genotypes while BALTNLM 17 was induced by ENU in a BALB/c mouse (Mathieson et al, 1978). They were all slightly hyperdiploid (Table IV).…”

“…The lymphoma BALTNLM 17, received from Dr. Michael Potter, was induced in a BALB/c mouse by i.p. injections of pristane and 1-ethyl-1-nitrosourea (Mathieson et al, 1978). The cell line thus obtained carries monomeric surface IgM (Jin Kim et al, 1979).…”

Subset derivation of T-cell acute lymphoblastic leukemia in man.

“…The longest survivor in this latter group lived for 19 mo after diagnosis. Prior studies ofmurine AKR strain T-cell lymphomas, induced with 1-ethyl-l-nitrosourea or spontaneously occurring, have shown these tumors to be either Lyl+ (helper cell phenotype) or Ly2,3+ (suppressor/cytotoxic cell phenotype) (34,35). In this regard, the restricted expression of subset specific Ly antigens in the case of murine T-cell tumors and TH2 antigens in the case of human T-ALL demonstrates obvious similarities between murine and human disease.…”

Animal models for IgE-meditated cancer immunotherapy

“…Ly-2 is a cell surface marker for murine CD8 cells that can also be expressed on tumors of T-cell origin [31, 32]. Cytotoxic T lymphocytes (CTL) play a key role in anti-tumor immunity, however, in certain cases tumors do not express cell surface proteins that allow CTL activation, which has been considered to be a reason for poor responses to CTL-mediated therapies [33].…”