Expression of m-Golsyn/Syntabulin gene during mouse brain development

Funakoshi, Eishi; Fukui, Masaki; Hamano, Ayako; Okamoto, Hiroshi; Sugiyama, Chie; Nishiyama, Norito; Ogita, Kiyokazu; Hori, Takamitsu; Shimizu, Nobuyoshi; Ito, F.

doi:10.1016/j.neulet.2006.04.061

Cited by 4 publications

(5 citation statements)

References 14 publications

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“…In this study, we used a yeast-two-hybrid screening system and identified a previously unrecognized PICK1-interacting protein, syntabulin. Syntabulin, which is involved in primary open-angle glaucoma 40 41 , is also named golsyn (human Golgi-localized syntaphilin-related protein) 42 . Syntabulin is a kinesin motor adaptor and it binds to syntaxin and mitochondria 24 25 26 43 .…”

Section: Discussionmentioning

confidence: 99%

Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons

Wang

Luo

et al. 2016

Sci Rep

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PICK1 (protein interacting with C-kinase 1) is a peripheral membrane protein that interacts with diverse membrane proteins. PICK1 has been shown to regulate the clustering and membrane localization of synaptic receptors such as AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptors, metabotropic glutamate receptor 7, and ASICs (acid-sensing ion channels). Moreover, recent evidence suggests that PICK1 can mediate the trafficking of various vesicles out from the Golgi complex in several cell systems, including neurons. However, how PICK1 affects vesicle-trafficking dynamics remains unexplored. Here, we show that PICK1 mediates vesicle trafficking by interacting with syntabulin, a kinesin-binding protein that mediates the trafficking of both synaptic vesicles and mitochondria in axons. Syntabulin recruits PICK1 onto microtubule structures and mediates the trafficking of PICK1-containing vesicles along microtubules. In neurons, syntabulin alters PICK1 expression by recruiting PICK1 into axons and regulates the trafficking dynamics of PICK1-containing vesicles. Furthermore, we show that syntabulin forms a complex with PICK1 and ASICs, regulates ASIC protein expression in neurons, and participates in ASIC-induced acidotoxicity.

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Section: Discussionmentioning

confidence: 99%

Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons

Wang

Luo

et al. 2016

Sci Rep

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“…Earlier, we demonstrated that Golsyn/Syntabulin is ubiquitously expressed in various mouse organs including the brain at mRNA and protein levels [16,17]. In the brain, nerve cells, but not glial cells, express Golsyn/Syntabulin; however, the cell types expressing Golsyn/Syntabulin in the organs other than the brain remained unclear.…”

Section: Discussionmentioning

confidence: 95%

“…2D and E). Golsyn/Syntabulin has been reported to be ubiquitously expressed in nerve cells of various brain regions of postnatal and adult mice [16,17]. It thus appears that this protein plays a significant role in nerve cells of the periphery in addition to those of the brain.…”

Section: Discussionmentioning

confidence: 99%

“…Later we isolated m-Golsyn, the mouse ortholog of human GOLSYN, and mapped it to the region on mouse chromosome 15B3.2 syntenic with human chromosome 8q23. m-Golsyn was expressed not only in neuronal cells but also in various organs and cell lines including epithelial cells [16,17]. Golsyn/Syntabulin, therefore, may be involved in vesicular transport in various organs other than the brain.…”

Section: Introductionmentioning

confidence: 99%

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High-Level Expression of Golsyn/Syntabulin in Glandular Epithelium and its Role in the Secretory Process~!2010-01-02~!2010-06-13~!2010-08-16~!

Oda¹,

Funakoshi²,

Ozaki³

et al. 2010

JEBP

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We recently identified a novel gene, named GOLSYN/Syntabulin, on human chromosome 8q23.2. In neurons, GOLSYN/Syntabulin functions as a molecule linking syntaxin 1-containing vesicles to kinesin, and thus plays an essential role in the transport of vesicles and mitochondria along neuronal processes. GOLSYN/Syntabulin is ubiquitously expressed in most peripheral tissues besides various brain regions. However, the physiological function in the periphery remains unknown. In this study, we determined the expression of Golsyn/Syntabulin in various mouse organs by immunohistochemical analysis using anti-GOLSYN antibody. Golsyn/Syntabulin was abundantly expressed in the glandular epithelium in the various peripheral organs examined: In exocrine glands, it was expressed in secretory cells, such as chief cells in the stomach and pancreatic acinar cells; and in endocrine glands, it was detected in pancreatic islet cells, which are endocrine cells that produce and release hormones including insulin and glucagon. The expression of Golsyn/Syntabulin was drastically decreased in pancreas and liver by fasting, but it was not affected in the testis, which has no secretory glands for food digestion. Subsequent refeeding recovered Golsyn/Syntabulin expression in pancreas and liver tissues to almost control levels, indicating the association of its expression with food intake. Further, administration of gastrin or pilocarpine led to a remarkable increase in the expression level of Golsyn/Syntabulin in the pancreas. Since food intake, gastrin, and pilocarpine are known to stimulate the secretion of pancreatic enzymes, Golsyn/Syntabulin may play an important role in the secretion process, including transport of secretory vesicles and/or exocytosis, in glandular epithelium.

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“…Syntabulin, as a molecular motor adapter, is a microtubuleassociated protein [11,12]. Syntabulin is expressed in the nervous system, including the mouse hippocampal cell layer [13].…”

Section: Introductionmentioning

confidence: 99%

Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B

Liu

et al. 2023

Cell Death Discov.

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Epilepsy is a widespread neurological disorder affecting more than 65 million people, but the mechanisms of epilepsy remains unknown. Abnormal synaptic transmission has a crucial role in the occurrence and development of epilepsy. Here, we found that syntabulin, a neuronal transporter, was mainly localized in neurons, and its expression was increased in epileptic tissues. Knockdown of syntabulin increased susceptibility and severity of epilepsy, whereas overexpression of syntabulin had the opposite effect. Mechanistically, in the epileptic brain tissue, syntabulin mainly translocated syntaxin 1B (STX1B) rather than syntaxin 1A (STX1A) to the presynaptic membrane, which resulted in increased presynaptic transmitter release. Further studies showed that syntabulin had a more significant effect on presynaptic functionality of GABAergic activity over that of excitatory synapses and resulted in an excitation/inhibition (E/I) imbalance, thereby regulating the epileptic phenotype. In addition, we found that the increased expression of syntabulin in epileptic brain tissue was mainly regulated by transcription factor TFAP2A. In summary, syntabulin plays a protective role in epilepsy by maintaining a proper E/I balance in the hippocampus.

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Expression of m-Golsyn/Syntabulin gene during mouse brain development

Cited by 4 publications

References 14 publications

Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons

Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons

High-Level Expression of Golsyn/Syntabulin in Glandular Epithelium and its Role in the Secretory Process~!2010-01-02~!2010-06-13~!2010-08-16~!

Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B

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