Expression of matrix metalloproteinases and components of the endocannabinoid system in the knee joint are associated with biphasic pain progression in a rat model of osteoarthritis

Pająk, Agnieszka; Kostrzewa, Magdalena; Małek, Natalia; Korostyński, Michał; Starowicz, Katarzyna

doi:10.2147/jpr.s132682

Cited by 23 publications

(20 citation statements)

References 56 publications

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“…MMP-9 protein expressions were higher in the synovial joint fluid of patients with OA [82], and it has been demonstrated to be highly expressed in the early stages of wound healing due to its involvement in angiogenesis [83]. Pajak et al (2017) [84] added that MMPs' genes transcription was increased in the osteoarthritic rat model, with the highest expression in mRNA for MMP-9, which was firstly increased at day 2 then significantly decreased at day 28 compared to the gradual increase of MMP-13 to day 28. These data in concomitant with our results reveal that gelatinases initiate the degradative sequences in early OA, followed by collagenases, which play a pronounced role in OA progression.…”

Section: Discussionmentioning

confidence: 99%

COL2A1 and Caspase-3 as Promising Biomarkers for Osteoarthritis Prognosis in an Equus asinus Model

et al. 2020

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Osteoarthritis (OA) is one of the most degenerative joint diseases in both human and veterinary medicine. The objective of the present study was the early diagnosis of OA in donkeys using a reliable grading of the disease based on clinical, chemical, and molecular alterations. OA was induced by intra-articular injection of 25 mg monoiodoacetate (MIA) as a single dose into the left radiocarpal joint of nine donkeys. Animals were clinically evaluated through the assessment of lameness score, radiographic, and ultrasonographic findings for seven months. Synovial fluid and cartilage samples were collected from both normal and diseased joints for the assessment of matrix metalloproteinases (MMPs) activity, COL2A1 protein expression level, and histopathological and immunohistochemical analysis of Caspase-3. Animals showed the highest lameness score post-induction after one week then decreased gradually with the progression of radiographical and ultrasonographic changes. MMP activity and COL2A1 and Caspase-3 expression increased, accompanied by articular cartilage degeneration and loss of proteoglycan. OA was successfully graded in Egyptian donkeys, with the promising use of COL2A1and Caspase-3 for prognosis. However, MMPs failed to discriminate between early and late grades of OA.

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Section: Discussionmentioning

confidence: 99%

COL2A1 and Caspase-3 as Promising Biomarkers for Osteoarthritis Prognosis in an Equus asinus Model

et al. 2020

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“… 10 , 11 Pajak et al further proved that the MIA model showed a biphasic progressive pattern of pain-like behaviors over 4 weeks. 8 In their study, an initial enormously increased weight-bearing deficit on day 2 after the MIA injection was followed by an alleviation of symptoms on days 6–12. On days 14–28, a second phase of chronic, progressive pain was observed.…”

Section: Introductionmentioning

confidence: 94%

Traumatic osteoarthritis-induced persistent mechanical hyperalgesia in a rat model of anterior cruciate ligament transection plus a medial meniscectomy

Tsai¹,

Chen²,

Chen³

et al. 2017

JPR

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BackgroundOsteoarthritis (OA) is a degenerative joint disease characterized by progressive cartilage degeneration, subchondral bone changes, osteophyte formation, and synovitis. A major symptom is pain that is triggered by peripheral and central changes within the pain pathways. Some surgery-induced joint instability rat models of OA were described to mimic traumatic OA. Several behavioral tests were developed to access OA-induced pain. However, follow-up in most studies usually only occurred for about 4 weeks. Since traumatic OA is a chronic disease which gradually develops after trauma, the pattern of pain might differ between early and late stages after the trauma.PurposeTo observe the time-dependent development of hypersensitivity after traumatic OA and to determine the best timing and methods to investigate traumatic OA-induced pain.MethodsAnterior cruciate ligament transection plus medial meniscectomy was used to induce traumatic OA in Sprague-Dawley rats. Traumatic OA-induced pain was evaluated using four different behavioral tests for 15 weeks.ResultsA significant difference in mechanical hypersensitivity developed throughout the observational period. It was worst in the first 3 weeks after the operation, then became less significant after 5 weeks but persisted. There were no differences in thermal hyperalgesia or motor coordination.ConclusionTraumatic OA induced mechanical hyperalgesia but did not cause thermal hyperalgesia or influence motor coordination. Furthermore, to investigate chronic pain induced by OA, the observational period should be at least 5 weeks after the intervention. These findings may help in further research and improve our understanding of traumatic OA-induced pain mechanisms.

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“…Bearing in mind the 3R rule for the ethical use of animals in testing, we decided not to repeat the behavioral experiments performed previously by our group. In the previous studies, a behavioral pattern of changes occurring during OA progression was precisely described [ 24 , 54 ]. Both papers characterized OA-related pain behavior by Pressure Application Measurement test (Ugo Basile, Italy) and Dynamic Weight Bearing test (Bioseb, France).…”

Section: Methodsmentioning

confidence: 99%

Alterations in Anandamide Synthesis and Degradation during Osteoarthritis Progression in an Animal Model

Bryk

Chwastek

Kostrzewa

et al. 2020

IJMS

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Osteoarthritis (OA) is a degenerative joint disease manifested by movement limitations and chronic pain. Endocannabinoid system (ECS) may modulate nociception via cannabinoid and TRPV1 receptors. The purpose of our study was to examine alterations in the spinal and joint endocannabinoid system during pain development in an animal model of OA. Wistar rats received intra-articular injection of 3mg of sodium monoiodoacetate (MIA) into the knee joint. Animals were sacrificed on day 2, 7, 14, 21, 28 after injection and lumbar spinal cord, cartilage and synovium were collected. Changes in the transcription levels of the ECS elements were measured. At the spinal level, gene expression levels of the cannabinoid and TRPV1 receptors as well as enzymes involved in anandamide synthesis and degradation were elevated in the advanced OA phase. In the joint, an important role of the synovium was demonstrated, since cartilage degeneration resulted in attenuation of the changes in the gene expression. Enzymes responsible for anandamide synthesis and degradation were upregulated particularly in the early stages of OA, presumably in response to early local joint inflammation. The presented study provides missing information about the MIA-induced OA model and encourages the development of a therapy focused on the molecular role of ECS.

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Expression of matrix metalloproteinases and components of the endocannabinoid system in the knee joint are associated with biphasic pain progression in a rat model of osteoarthritis

Cited by 23 publications

References 56 publications

COL2A1 and Caspase-3 as Promising Biomarkers for Osteoarthritis Prognosis in an Equus asinus Model

COL2A1 and Caspase-3 as Promising Biomarkers for Osteoarthritis Prognosis in an Equus asinus Model

Traumatic osteoarthritis-induced persistent mechanical hyperalgesia in a rat model of anterior cruciate ligament transection plus a medial meniscectomy

Alterations in Anandamide Synthesis and Degradation during Osteoarthritis Progression in an Animal Model

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