Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine

Kim, Sang‐Hwan; Lee, Ji Hye; Yoon, Jong Taek

doi:10.7717/peerj.6344

Cited by 8 publications

(7 citation statements)

References 34 publications

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“…We therefore speculated that UPR may be involved in the process of goat luteal cells dividing from follicular granulosa cells and theca cells. The regression of the CL occurs in the late luteal phase of the goat CL and is accompanied with the apoptosis of the luteal cells stimulated by PGF2α (Tanaka et al, 2000;Diaz et al, 2002;Luttgenau et al, 2016;Kim et al, 2019). A previous study revealed that the caspase-dependent apoptosis pathway was significantly activated in bovine CL after PGF2α-injection (Kliem et al, 2009).…”

Section: Discussionmentioning

confidence: 98%

Prostaglandin F2α Induces Goat Corpus Luteum Regression via Endoplasmic Reticulum Stress and Autophagy

Wen

Liu

et al. 2020

Front. Physiol.

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Corpus luteum (CL) is a transient endocrine tissue that produces progesterone for maintaining pregnancy in mammals. In addition, the regression of CL is necessary for the initiation of the estrous cycle. Extensive research has shown that the prostaglandin F2α (PGF2α) induces the regression of CL in ruminants. However, the mechanisms of endoplasmic reticulum (ER) stress and autophagy in the regression of goat CL induced by PGF2α are still unclear. In this study, ovaries of dioestrus goats and goats that were 3 months pregnant were collected to detect the location of the ER stress-related protein GRP78. The relationship between the different stages of the luteal phase of goat CL during the estrous cycle and changes in the expression of ER stress-related proteins and autophagy-related proteins was confirmed by western blot analysis. The results showed that both ER stress and autophagy were activated in the late luteal phase of the goat CL. To reveal the function of ER stress and autophagy in the CL regression process induced by PGF2α, we used 4-phenyl butyric acid (4-PBA) and chloroquine (CQ) for inhibiting ER stress and autophagy, respectively. Through the apoptotic rate detected by the flow cytometry and the expression of ER stress-and autophagy-related proteins detected by western blotting, we demonstrated that ER stress promoted goat luteal cell apoptosis and autophagy, and that apoptosis can be enhanced by the inhibition of autophagy. In addition, knockdown of EIF2S1, which blocked the PERK pathway activation, promoted apoptosis by reducing autophagy in goat luteal cells treated with PGF2α. In conclusion, our study indicates that ER stress promotes goat luteal cell apoptosis to regulate the regression of CL and activates autophagy to inhibit the goat luteal cell apoptosis via PERK signaling pathway.

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Section: Discussionmentioning

confidence: 98%

Prostaglandin F2α Induces Goat Corpus Luteum Regression via Endoplasmic Reticulum Stress and Autophagy

Wen

Liu

et al. 2020

Front. Physiol.

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“…Structural changes in the corpus luteum can be observed in the rapid reorganisation of tissues during the process of a mammal becoming fertile during the oestrous cycle. In the process of tissue reorganisation, functional changes involve cytoplasmic reorganisation and changes in the role of cells the apoptosis mechanism (14). However, the main functional changes in cells can be observed simultaneously in apoptosis and cytoplasmic changes.…”

Section: Discussionmentioning

confidence: 99%

“…The maintenance of LH and the difference in expression of 20α-HSD may have a certain influence on cell reorganisation because of the difference in expression of MMPs and TIMPs. However, it is not known whether these differences regulate the two processes of apoptosis (13,14,16). The regulation of autophagy has not yet been elucidated, and further studies are needed to understand the interaction of several components (15).…”

Section: Discussionmentioning

confidence: 99%

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Functional and morphological maturation of the full-sized and mini-pig corpus luteum by programmed cell death mechanism

Lee

Kim

2023

Journal of Veterinary Research

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Introduction The formation and function of the corpus luteum (CL) increase the likelihood of pregnancy and efficiently manage implantation. Apoptosis must occur at an appropriate time in the formation of the CL. This also affects its function. However, it is still unclear if the type of apoptosis affects the function. Material and Methods We conducted morphological analysis of the CL collected on day 15 between the middle and late oestrous phases of Yorkshire pigs and mini-pigs, and measured the difference in hormone expression and apoptosis using an immunoassay method and messenger RNA level. Results The CL cells were more uniform in the Yorkshire pigs than in the mini-pigs, and the composition of the CL was also fuller. The expression of luteinising hormone was higher in the Yorkshire pigs. Apoptosis and the rate of action of matrix metalloproteinases (MMPs) were different between the two pig types. Expression of MMPs was higher in the Yorkshire pigs than in the mini-pigs. However, the expression of caspase 3 and 20alpha-hydroxysteroid dehydrogenase, a progesterone inhibitor, was potentiated in the mini-pigs. Conclusion Autophagy throughout the CL was more extensive in the Yorkshire pigs than in the mini-pigs, suggesting that autophagy and cell reorganisation by MMPs were highly correlated. The occurrence of autophagy in the formation and function of the CL may affect the action of hormones and expression of cell reconstitution factors.

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“…The remodeling process involves the production and activation of various ECM-degrading enzymes, such as matrix metalloproteinases (MMPs). These enzymes facilitate the breakdown of the ECM and contribute to luteal cell apoptosis [81]. Additionally, cytokines and chemokines released by immune cells can modulate this [82].…”

Section: Extracellular Factorsmentioning

confidence: 99%

Angiogenesis and Apoptosis: Data Comparison of Similar Microenvironments in the Corpus Luteum and Tumors

Min,

Lee,

Lee

2024

Animals

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The corpus luteum is a temporary endocrine gland formed in the ovary after ovulation, and it plays a critical role in animal reproductive processes. Tumors rely on the development of an adequate blood supply to ensure the delivery of nutrients and oxygen and the removal of waste products. While angiogenesis occurs in various physiological and pathological contexts, the corpus luteum and tumors share similarities in terms of the signaling pathways that promote angiogenesis. In the corpus luteum and tumors, apoptosis plays a crucial role in controlling cell numbers and ensuring proper tissue development and function. Interestingly, there are similarities between the apoptotic-regulated signaling pathways involved in apoptosis in the corpus luteum and tumors. However, the regulation of apoptosis in both can differ due to their distinct physiological and pathological characteristics. Thus, we reviewed the biological events of the corpus luteum and tumors in similar microenvironments of angiogenesis and apoptosis.

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Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine

Cited by 8 publications

References 34 publications

Prostaglandin F2α Induces Goat Corpus Luteum Regression via Endoplasmic Reticulum Stress and Autophagy

Prostaglandin F2α Induces Goat Corpus Luteum Regression via Endoplasmic Reticulum Stress and Autophagy

Functional and morphological maturation of the full-sized and mini-pig corpus luteum by programmed cell death mechanism

Angiogenesis and Apoptosis: Data Comparison of Similar Microenvironments in the Corpus Luteum and Tumors

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