Expression of Mcm2, geminin and Ki67 in normal oral mucosa, oral epithelial dysplasias and their corresponding squamous-cell carcinomas

Torres-Rendon, A.; Roy, Sushmita; Craig, Gavin A.; Speight, Paul M.

doi:10.1038/sj.bjc.6604967

Cited by 105 publications

(112 citation statements)

References 30 publications

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“…It is present in S, G2 and M phases of the cell cycle, but absent in the G1 phase (20,21). Numerous previous studies examined the effect of Geminin expression on the overall survival of patients with breast, colorectal and prostatic cancers and showed that a higher expression of Geminin was a poor prognostic marker in patients with these types of cancer (33)(34)(35)37,38). On the other hand, Shrestha et al found that the relationship between Geminin expression and survival in patients with high-grade astrocytic tumors contradicted the previous findings (36), and that the overexpression of Geminin was associated with a longer survival of patients with high-grade astrocytic tumors.…”

Section: Discussionmentioning

confidence: 99%

“…The present study shows the prognostic significance of MCM7, Geminin and Ki-67, using immunohistochemical methods in 109 STSs. MCM proteins were reported to be correlated with the histologic grade in other types of cancer, including prostate cancer, lung adenocarcinoma and renal cell carcinoma (27)(28)(29)(30)(31)(32)(33)(34). We previously reported the positive relation between the expression of MCM2 and the prognosis of patients with human malignant fibrous histiocytoma (19).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, MCM7 was considered a useful proliferation marker in colonic, prostate, endometrial and cervical cancers (30,31). In addition, Geminin is overexpressed in breast, colorectal and prostate cancers (33)(34)(35)(36)(37)(38). However, the clinicopathological importance of MCM7 and Geminin expression in STSs has yet to be thoroughly investigated.…”

Section: Introductionmentioning

confidence: 99%

“…MCM proteins were suggested as potential prognostic markers in a variety of human malignancies, including prostate and breast cancer and bronchial adenocarcinoma (19,(27)(28)(29)(30)(31)(32)(33)(34). Overexpression of Minichromosome maintenance protein 7 (MCM7) was reported to actively contribute to tumor formation, progression and malignant conversion.…”

Section: Introductionmentioning

confidence: 99%

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Prognostic significance of Minichromosome maintenance protein 7 and Geminin expression in patients with 109 soft tissue sarcomas

Hamamoto

Shomori²,

Nosaka³

et al. 2010

Oncology Letters

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Abstract. Minichromosome maintenance complex (MCM2-7)and Geminin are important in the prevention of DNA re-replication in the cell cycle, and are also prognostic markers for numerous human malignancies. The present study examined Minichromosome maintenance protein 7 (MCM7) and Geminin expression in human soft tissue sarcomas (STSs) to clarify their correlation to the clinicopathological factors. Immunohistochemistry was performed to detect the expression of MCM7, Geminin and Ki-67 on paraffin-embedded sections of 109 STSs. Labeling indices (LIs) of the molecules were evaluated in the tumors. Higher LIs of MCM7, Geminin and Ki-67 were significantly correlated with distant metastasis (P<0.01), histological grade (P<0.01) and poor prognosis (P<0.01), respectively. LIs of MCM7 and Geminin were significantly correlated with Ki-67 LIs, (MCM7/Ki-67: rs=0.745, P<0.01 and Geminin/Ki-67: rs=0.604, P<0.01). Multivariate analyses showed that the higher LIs of Geminin, but not MCM7 and Ki-67, were shown to be an independent factor of poorer prognosis (relative risk 2.72, P=0.013). The immunohistochemical expression of MCM7 and Geminin may be novel and useful markers for evaluating the prognosis in patients with human STS.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prognostic significance of Minichromosome maintenance protein 7 and Geminin expression in patients with 109 soft tissue sarcomas

Hamamoto

Shomori²,

Nosaka³

et al. 2010

Oncology Letters

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“…After DNA replication is initiated during the cell cycle, Geminin inhibits re-uploading of the MCM proteins onto chromatin, thus preventing DNA re-replication in the same cell cycle (13). It has been confirmed that these proteins are useful biomakers that reflect the proliferative activity of the tumor cells and predict the survival of patients Multiparameter analysis using cell cycle biomarkers for small-size lung adenocarcinoma : Prognostic implications TOMOHIRO HARUKI 1,2 , KOHEI SHOMORI 1 , TATSUSHI SHIOMI 1 , YUJI TANIGUCHI 2 , HIROSHIGE NAKAMURA 2 and HISAO ITO 1 1 with various types of cancer (5)(6)(7)(8)(9)(10)(11)(14)(15)(16)(17)(18). Aurora A, known as a member of the serine/threonine kinase family, controls a subset of critical mitotic events including centrosome maturation and separation, as well as chromosome orientation and segregation (19).…”

Section: Introductionmentioning

confidence: 99%

Multiparameter analysis using cell cycle biomarkers for small-size lung adenocarcinoma: prognostic implications

et al. 2012

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Abstract. Cell cycle-related molecules play crucial roles in maintaining genomic stability, and can also serve as biomarkers of cell cycle phase distribution at the same time. In this study, we used multiparameter analysis of various biomarkers to investigate their utility for the evaluation of tumor proliferation activities and the prognosis of patients with small-size lung adenocarcinoma. We performed immunohistochemical analysis using five cell cycle-related biomarkers (MCM7, Ki-67, Geminin, Aurora A and H3S10ph) for 102 surgically resected small-size lung adenocarcinomas. We classified them into three phenotypes based on the dominant cell cycle phase distribution of the tumor cell population, and evaluated whether these phenotypes were associated with clinicopathological factors and survival. Phenotype I (MCM7-negative tumors; n=56) was correlated with high or moderate differentiation and reduced local invasiveness (pleural and lymphovascular invasion) compared with phenotype II (MCM7-, Ki-67-and Geminin-positive tumors; n=23) and phenotype III (MCM7-, Aurora A-and H3S10ph-positive tumors; n=17). Five-year survival rates of phenotypes I, II and III were 89.8, 55.4 and 38.6%, respectively, with a significant difference between them (P<0.01). Multivariate analysis revealed that phenotypes II and III were independent prognostic factors in the 79 patients with stage I lung adenocarcinoma. Multiparameter analysis using cell cycle biomarkers for small-size lung adenocarcinoma provided novel insights into the cell cycle phase distribution of dynamic tumor cell populations in vivo; it may be possible to evaluate tumor proliferation activities and patient prognosis more precisely if this analytical procedure is used.

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Point‐of‐care oral cytology tool for the screening and assessment of potentially malignant oral lesions

McRae

Modak

Simmons

et al. 2020

Cancer Cytopathology

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Background The effective detection and monitoring of potentially malignant oral lesions (PMOL) are critical to identifying early‐stage cancer and improving outcomes. In the current study, the authors described cytopathology tools, including machine learning algorithms, clinical algorithms, and test reports developed to assist pathologists and clinicians with PMOL evaluation. Methods Data were acquired from a multisite clinical validation study of 999 subjects with PMOLs and oral squamous cell carcinoma (OSCC) using a cytology‐on‐a‐chip approach. A machine learning model was trained to recognize and quantify the distributions of 4 cell phenotypes. A least absolute shrinkage and selection operator (lasso) logistic regression model was trained to distinguish PMOLs and cancer across a spectrum of histopathologic diagnoses ranging from benign, to increasing grades of oral epithelial dysplasia (OED), to OSCC using demographics, lesion characteristics, and cell phenotypes. Cytopathology software was developed to assist pathologists in reviewing brush cytology test results, including high‐content cell analyses, data visualization tools, and results reporting. Results Cell phenotypes were determined accurately through an automated cytological assay and machine learning approach (99.3% accuracy). Significant differences in cell phenotype distributions across diagnostic categories were found in 3 phenotypes (type 1 [“mature squamous”], type 2 [“small round”], and type 3 [“leukocytes”]). The clinical algorithms resulted in acceptable performance characteristics (area under the curve of 0.81 for benign vs mild dysplasia and 0.95 for benign vs malignancy). Conclusions These new cytopathology tools represent a practical solution for rapid PMOL assessment, with the potential to facilitate screening and longitudinal monitoring in primary, secondary, and tertiary clinical care settings.

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Expression of Mcm2, geminin and Ki67 in normal oral mucosa, oral epithelial dysplasias and their corresponding squamous-cell carcinomas

Cited by 105 publications

References 30 publications

Prognostic significance of Minichromosome maintenance protein 7 and Geminin expression in patients with 109 soft tissue sarcomas

Prognostic significance of Minichromosome maintenance protein 7 and Geminin expression in patients with 109 soft tissue sarcomas

Multiparameter analysis using cell cycle biomarkers for small-size lung adenocarcinoma: prognostic implications

Point‐of‐care oral cytology tool for the screening and assessment of potentially malignant oral lesions

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