Expression of MCT1, MCT2 and MCT4 in the rumen, small intestine and liver of reindeer (Rangifer tarandus tarandus L.)

Koho, Ninna; Maijala, Veikko; Norberg, Harri; Nieminen, Mauri; Pösö, A.R.

doi:10.1016/j.cbpb.2005.03.003

Cited by 33 publications

(43 citation statements)

References 26 publications

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“…The low oxidative capacity, sparse capillarization, and fiber-type distribution of the muscles of the domestic pig render it an interesting animal for studies of MCT isoform composition. In the gastrointestinal tract, MCTs are important in the transport of short-chain fatty acids (SCFAs) and lactate (Koho et al 2005). SCFAs are weak acids, but because the pH of the gastrointestinal tract, with the exception of the stomach, is nearly neutral, 90-99 % of SCFAs are present as anions rather than as free acids.…”

Section: Introductionmentioning

confidence: 99%

A comparative study on the expression profile of MCTs and HSPs in Ghungroo and Large White Yorkshire breeds of pigs during different seasons

Parkunan

Banerjee

Mohanty

et al. 2015

Cell Stress and Chaperones

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Thermal stress has a significant adverse effect on commercial swine production but it is not easy to measure. Animals may adapt to stress conditions by an alteration in the expression of stress-related genes such as heat shock proteins (HSPs) and monocarboxylate transporters (MCTs). The present study presents a comparative analysis of seasonally varied effects on the expression profiles of HSPs (27, 70, and 90) and MCTs (1, 2, and 4) transcripts in thigh muscle and colon tissue of Ghungroo and Large White Yorkshire (LWY) breeds of pig. By real-time polymerase chain reaction, the mRNA expression of HSP27 and HSP90 genes was found to be higher in both thigh muscle and colon tissue in Ghungroo compared to Large White Yorkshire pigs during the summer. However, the relative expression of HSP70 was significantly higher (P<0.01) in Ghungroo compared to Large White Yorkshire pigs during both seasons in both thigh muscle and colon tissue. The expression of HSP90 was higher in Ghungroo when compared to LWY though the variation was non-significant (P>0.05) in the colon during different seasons. However, in Ghungroo, the mRNA expression of MCT1 was found to be significantly (P<0.05) higher in thigh muscle and colon regions during the summer compared to LWY, whereas MCT2 was expressed more in the colon in LWY compared to Ghungroo during the summer. The relative expression of mRNA of MCT4 was found to be significantly (P<0.05) higher in thigh region in both summer and winter in Ghungroo compared with LWY. Thus, the study demonstrated that both HSPs and MCTs gene expression during thermal stress suggests the possible involvement of these genes in reducing the deleterious effect of thermal stress, thus maintaining cellular integrity and homeostasis in pigs. These genes could be used as suitable markers for the assessment of stress in pigs.

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Section: Introductionmentioning

confidence: 99%

A comparative study on the expression profile of MCTs and HSPs in Ghungroo and Large White Yorkshire breeds of pigs during different seasons

Parkunan

Banerjee

Mohanty

et al. 2015

Cell Stress and Chaperones

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“…The transport of these metabolites from rumen epithelium to the blood is facilitated by a large-conductance anion channel in the basolateral membrane (Stumpff et al 2009) and by the Monocarboxylate transporter 1 (MCT1). The carrier is known to be dominantly located in the cell membrane of REC from the stratum basale (SB) , Taifor et al 2009 and to mediate a cotransport of its substrates (ketone bodies, lactate, pyruvate) with protons , Koho et al 2005, Kirat et al 2006, Graham et al 2007). Thus, MCT1 possesses an important role in transepithelial transfer of nutrients and in rumen pH regulation , Gäbel & Aschenbach 2006, Kirat et al 2005, Kirat et al 2006.…”

Section: Introductionmentioning

confidence: 99%

The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay <i>ad libitum</i> diet (Short Communication)

Kuzinski

Röntgen

2011

Arch. Anim. Breed.

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In this study the protein and mRNA expression of Monocarboxylate transporter 1 (MCT1) was evaluated in rumen epithelial cells (REC) obtained from sheep fed hay ad libitum (control, h diet, n=4) or a mixed hay/concentrate diet (h/c diet, n=4) for two weeks. REC were isolated via fractionated trypsination and three groups consisting of fractions 3 to 5=G1, fractions 6 to 8=G2, and fractions 9 and 10=G3 were formed. Using an anti-basal cytokeratin antibody and flow cytometric analysis, the proportion of REC originating from the stratum basale (SB) was determined for each group. In addition, MCT1 mRNA and protein expression was determined by qRT-PCR and Western blot, respectively. Feeding the h/c diet led to a 299±93 % elevation of the number of SB cells known to express the MCT1 protein. This is accompanied by an increased MCT1 mRNA (1.8 to 2.2-fold) and protein (1.3-fold) expression. Thus, an increased number of MCT1 expressing cells and upregulation of ruminal MCT1 protein seem to be components of rumen epithelium functional adaptation to high energy diet.Keywords: sheep, rumen, epithelial cells, transport protein, high energy diet Zusammenfassung Die mRNA-und Protein-Expression des MCT1 in Pansenepithelzellen wird durch Zufütterung von Konzentrat zu einer ad libitum Heu-Ration erhöhtIn der vorliegenden Studie wurden die mRNA-und Proteinexpression des Monocarboxylattransporters 1 (MCT1) in Pansenepithelzellen (PEZ) von Schafen, die über zwei Wochen entweder Heu ad libitum (h-Diät, n=4) oder eine Heu/Konzentrat-Mischration (h/c-Diät, n=4) erhielten, untersucht. Ovine PEZ wurden mittels fraktionierter Trypsinierung gewonnen und anschließend drei Zellgruppen, bestehend aus den Fraktionen 3-5 (G1), 6-8 (G2) und 9/10 (G3), zugeordnet. Mittels Flowzytometrie und unter Nutzung eines spezifischen Antikörpers gegen basales Cytokeratin wurde der Anteil von PEZ aus dem Stratum basale (SB) für jede Zellgruppe ermittelt. Die Expression von MCT1-mRNA und -Protein wurde mittels qRT-PCR und Western Blot analysiert. Die Anzahl MCT1-exprimierender SB-Zellen, stieg nach Füt-terung der h/c-Diät um 299±93 %. Damit ist eine Erhöhung der MCT1-mRNA-(1,8-2,2-fach) sowie der Proteinexpression (1,3-fach) verbunden. Ein erhöhter Anteil MCT1-expremierender PEZ und die Aufregulation der MCT1 Proteinmenge scheinen somit Komponenten der funktionellen Anpassung des Pansenepithels an energiereiche Fütterung darzustellen.

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“…There is data available that may provide some clues concerning the tissue distribution of the MCT1 protein in many animal species, including the hamster gastrointestinal tract [6,7], rabbit small intestine [29], rat gastrointestinal tract [28], pig colon [24], sheep rumen [19], bovine gastrointestinal tract [14], ovine gastrointestinal tract [15], reindeer small intestine [16], and human colon [24] and small intestine [8]. The distribution is somewhat different between ruminants and monogastric animals.…”

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confidence: 99%

Expression of Monocarboxylate Transporter 1 (MCT1) in the Dog Intestine

Shimoyama

Kirat

Akihara

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. In this study, the expression and distribution of monocarboxyolate transporter 1 (MCT1) along the intestines (duodenum, jejunum, ileum, cecum, colon and rectum) of dogs were investigated at both the mRNA and protein levels. The expression of MCT1 protein and its distribution were confirmed by Western blotting and immunohistochemical staining using the antibody for MCT1. We identified mRNA coding for MCT1 and a 43-kDa band of MCT1 protein in all regions from the duodenum to the rectum. Immunoreactive staining for MCT1 was also observed in epithelial cells throughout the intestines. MCT1 immunoreactivity was greater in the large intestine than in the small intestine. MCT1 protein was predominantly expressed on the basolateral membranes along intestinal epithelial cells, suggesting that MCT1 may play an important role in lactate efflux and transport of short-chain fatty acids (SCFAs) to the bloodstream across the basolateral membranes of the dog intestine. KEY WORDS: canine, intestine, monocarboxylate transporter 1.

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Expression of MCT1, MCT2 and MCT4 in the rumen, small intestine and liver of reindeer (Rangifer tarandus tarandus L.)

Cited by 33 publications

References 26 publications

A comparative study on the expression profile of MCTs and HSPs in Ghungroo and Large White Yorkshire breeds of pigs during different seasons

A comparative study on the expression profile of MCTs and HSPs in Ghungroo and Large White Yorkshire breeds of pigs during different seasons

The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay <i>ad libitum</i> diet (Short Communication)

Expression of Monocarboxylate Transporter 1 (MCT1) in the Dog Intestine

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Expression of MCT1, MCT2 and MCT4 in the rumen, small intestine and liver of reindeer (Rangifer tarandus tarandus L.)

Cited by 33 publications

References 26 publications

A comparative study on the expression profile of MCTs and HSPs in Ghungroo and Large White Yorkshire breeds of pigs during different seasons

A comparative study on the expression profile of MCTs and HSPs in Ghungroo and Large White Yorkshire breeds of pigs during different seasons

The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay &lt;i&gt;ad libitum&lt;/i&gt; diet (Short Communication)

Expression of Monocarboxylate Transporter 1 (MCT1) in the Dog Intestine

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The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay <i>ad libitum</i> diet (Short Communication)