Expression of melittin gene in the venom gland
of the Chinese honeybee, <i>Apis cerana cerana</i>

Li, Jianghong; Zhang, Chuan‐Xi; Tang, Zhengyi

doi:10.1051/apido:2005040

Cited by 3 publications

(3 citation statements)

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“…Similar observations were also made on other venom components such as melittin (Owen and Pfaff, 1995;Li et al, 2005). Moreover, it has been documented that the total venom content in A. mellifera and A. cerana was 138 and 43 µg, respectively (Schmidt, 1995).…”

Section: Discussionsupporting

confidence: 78%

“…The quantity of PLA 2 in the venom sac of A. cerana was 10-12 µg per worker, much less than that in A. mellifera, which was 30-40 µg per worker (Owen et al, 1990). Similar observations were also made on other venom components such as melittin (Owen and Pfaff, 1995;Li et al, 2005). Moreover, it has been documented that the total venom content in A. mellifera and A. cerana was 138 and 43 µg, respectively (Schmidt, 1995).…”

Section: Discussionsupporting

confidence: 60%

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Expression and regulation of phospholipase A2 in venom gland of the chinese honeybee,Apis cerana cerana

Zhang

Shen

et al. 2005

Arch. Insect Biochem. Physiol.

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Phospholipase A(2) (PLA(2)) is one of the components of bee venom with a wide range of pharmacological functions. It operates as a major allergen working with other venom components to defend the colony from intruder. In the present study, the cDNA sequence of the Ac-pla(2) gene from cDNA library of the venom gland of Apis cerana was compared with the amplified corresponding region of genomic DNA. The result showed that the Ac-pla(2) gene consisted of four exons and three introns. Southern blot showed that the Ac-pla(2) gene was a single copy per haploid genome. The most active transcription period was during the first 8 days of adults, which correspondingly was the period of sharp increase of PLA(2) protein. ELISA analysis revealed that the PLA(2) was undetectable in pupal stage and the newly eclosed adult, but increased sharply to a maximum of 10-12 mug per honeybee by 8-10 days of adult life, followed by a gradual decrease to 8 mug for the rest of adult life. Transcriptional or post transcriptional regulation is the key step for Ac-pla(2) expression. The early secreted Ac-PLA(2) showed a low degree of post-translational modification; with increasing age, glycosylation was detected by Western blot and glycoprotein staining analysis. Different post-translational modifications were found among different individuals in A. cerana when compared to A. mellifera.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 60%

Expression and regulation of phospholipase A2 in venom gland of the chinese honeybee,Apis cerana cerana

Zhang

Shen

et al. 2005

Arch. Insect Biochem. Physiol.

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“…Bee venom or Apitoxin, a biotoxin produced by the Apis species, is a complex mixture of several biologically active substances including enzymes, bioactive amines, peptides and non-peptides. Among these substances, melittin is the principal component in bee venom at approximately 40–50% of the dry weight of the venom [ 10 , 14 , 22 ]. Moreover, melittin in the bee venom of Apis mellifera (European honey bee), a common honey bee found worldwide, has been extensively studied [ 11 , 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment

et al. 2020

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Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was investigated against human malignant melanoma (A375) cells. In this study, Mel-AF exhibited considerable potential in the anti-proliferative action of A375 cells. Subsequently, the cellular mechanism of Mel-AF that induced cell death was investigated in terms of apoptosis. As a result, gene and protein expression levels, which indicated the activation of cytochrome-c release and caspase-9 expression, eventually triggered the release of the caspase-3 executioner upon Mel-AF. We then determined that apoptosis-mediated cell death was carried out through the intrinsic mitochondrial pathway. Moreover, advanced abilities, including cell motility and invasion, were significantly suppressed. Mel-AF manipulated the actin arrangement via the trapping of stress fibers that were found underneath the membrane, which resulted in the defective actin cytoskeleton organization. Consequently, the expression of EGFR, a binding protein to F-actin, was also found to be suppressed. This outcome strongly supports the effects of Mel-AF in the inhibition of progressive malignant activity through the disruption of actin cytoskeleton-EGFR interaction and the EGFR signaling system. Thus, the findings of our current study indicate the potential usefulness of Mel-AF in cancer treatments as an apoptosis inducer and a potential actin-targeting agent.

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Risques et valorisation des insectes dans l’alimentation humaine et animale

Megido

Alabi

Larreché³

et al. 2015

Annales de la Société entomologique de France (N.S.)

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Expression of melittin gene in the venom gland of the Chinese honeybee, Apis cerana cerana

Cited by 3 publications

References 26 publications

Expression and regulation of phospholipase A2 in venom gland of the chinese honeybee,Apis cerana cerana

Expression and regulation of phospholipase A2 in venom gland of the chinese honeybee,Apis cerana cerana

Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment

Risques et valorisation des insectes dans l’alimentation humaine et animale

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