Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells

Muhammed, Aisha; Yunus, Mohd.; Saim, Aminuddin Bin; Idrus, Ruszymah Bt Hj

doi:10.4067/s0717-95022015000400047

Cited by 2 publications

(3 citation statements)

References 14 publications

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“…In contrast, nasal organoids bring the possibility to perform high-throughput screening ( Phan et al, 2019 ; Mead et al, 2020 preprint). Furthermore, evaluation of the expression levels of the mesenchymal stem cell markers CD73, CD90, CD105 and the hematopoietic stem cell marker CD45 in HNECs (monolayers) showed that the quality of the cells was maintained up to passage four ( Muhammed et al, 2015 ). In contrast, HNECs when cultured under 3D-spheroid-forming conditions can be maintained for many generations.…”

Section: Discussionmentioning

confidence: 99%

“…Although both model systems have contributed enormously to the study of the pathophysiology of CRS, they also have their limitations. In order to get sufficient cells for experimental inquiry, HNECs have to be cultured for multiple passages, however, HNECs only retain their properties up to passage four, after which they go into senescence ( Muhammed et al, 2015 ). Moreover, as for any primary cell, HNECs vary in their response to different challenges and thus experiments have to be repeated using cells from different donors.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of human nasal organoids from chronic rhinosinusitis patients

et al. 2022

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Patient-derived organoids grown in three-dimensional cultures provide an excellent platform for phenotypic high-throughput screening and drug response research. Organoid technology has been applied to study stem cell biology and various human pathologies. This study investigates the characteristics and cellular morphology of organoids derived from primary human nasal epithelial cells (HNECs) of chronic rhinosinusitis (CRS) patients. Nasal organoids were cultured up to 20 days and morphological, cell composition, and functional parameters were measured by immunofluorescence, RT-qPCR, western-blot and FACS analysis. The results showed that nasal organoids expressed the stem cell marker Leucine Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5), and markers for apical junction genes, goblet cells and ciliated cells. Moreover, we were able to regrow and expand the nasal organoids well after freezing and thawing. This study promise an effective and feasible method for development of human nasal organoids, suitable for the phenotypic high-throughput screening and drug response research.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of human nasal organoids from chronic rhinosinusitis patients

et al. 2022

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“…Primary cells harvested from the nasal epithelium are often used for research, but these cells have slower growth speed than cell lines. Human nasal epithelial cells (HNECs) only retain their properties up to passage four after which they go into senescence ( 16 ). Therefore, the cells are not applicable for experiments involving knockdown, which is one of the basic methods to investigate the molecular mechanisms within cells.…”

Section: Tlrs In the Upper Airwaymentioning

confidence: 99%

TLR Signals in Epithelial Cells in the Nasal Cavity and Paranasal Sinuses

et al. 2021

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The respiratory tract is constantly at risk of invasion by microorganisms such as bacteria, viruses, and fungi. In particular, the mucosal epithelium of the nasal cavity and paranasal sinuses is at the very forefront of the battles between the host and the invading pathogens. Recent studies have revealed that the epithelium not only constitutes a physical barrier but also takes an essential role in the activation of the immune system. One of the mechanisms equipped in the epithelium to fight against microorganisms is the Toll-like receptor (TLR) response. TLRs recognize common structural components of microorganisms and activate the innate immune system, resulting in the production of a plethora of cytokines and chemokines in the response against microbes. As the epithelia-derived cytokines are deeply involved in the pathogenesis of inflammatory conditions in the nasal cavity and paranasal sinuses, such as chronic rhinosinusitis (CRS) and allergic rhinitis (AR), the molecules involved in the TLR response may be utilized as therapeutic targets for these diseases. There are several differences in the TLR response between nasal and bronchial epithelial cells, and knowledge of the TLR signals in the upper airway is sparse compared to that in the lower airway. In this review, we provide recent evidence on TLR signaling in the upper airway, focusing on the expression, regulation, and responsiveness of TLRs in human nasal epithelial cells (HNECs). We also discuss how TLRs in the epithelium are involved in the pathogenesis of, and possible therapeutic targeting, for CRS and AR.

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Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells

Cited by 2 publications

References 14 publications

Characterization of human nasal organoids from chronic rhinosinusitis patients

Characterization of human nasal organoids from chronic rhinosinusitis patients

TLR Signals in Epithelial Cells in the Nasal Cavity and Paranasal Sinuses

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