Expression of messenger RNAs of luteinizing hormone and follicle-stimulating hormone receptors in the granulosa layer during the ovulatory cycle of the hen

Yamamura, Namiko; Takeishi, Masaru; Goto, Nobuaki; Tagami, Minoru; Mizutani, Tetsuya; Miyamoto, Kaoru; Doi, Osamu; Kamiyoshi, Michiharu

doi:10.1080/00071660050149047

Cited by 2 publications

(3 citation statements)

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“…Regulation of meiotic initiation (Krentz et al, 2011;Zhang et al, 2015) spo11 Involved in meiotic recombination (Guioli et al, 2014;Oréal, Mazaud, Picard, Magre, & Carré-Eusèbe, 2002) Sertoli cells activity (spermatogenesis) fshr Gonadotropin receptor, stimulates spermatogenesis (Akazome, Abe, & Mori, 2002;Yamamura et al, 2000) wt1…”

Section: Germ Cells Development Stra8mentioning

confidence: 99%

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Dose‐response effects of light at night on the reproductive physiology of great tits (Parus major): Integrating morphological analyses with candidate gene expression

et al. 2018

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Artificial light at night (ALAN) is increasingly recognized as a potential threat to wildlife and ecosystem health. Among the ecological effects of ALAN, changes in reproductive timing are frequently reported, but the mechanisms underlying this relationship are still poorly understood. Here, we experimentally investigated these mechanisms by assessing dose‐dependent photoperiodic responses to ALAN in the great tit (Parus major). We individually exposed photosensitive male birds to one of three nocturnal light levels (0.5, 1.5, and 5 lux), or to a dark control. Subsequent histological and molecular analyses on their testes indicated a dose‐dependent reproductive response to ALAN. Specifically, different stages of gonadal growth were activated after exposure to different levels of light at night. mRNA transcript levels of genes linked to the development of germ cells (stra8 and spo11) were increased under 0.5 lux compared to the dark control. The 0.5 and 1.5 lux groups showed slight increases in testis size and transcript levels associated with steroid synthesis (lhr and hsd3b1) and spermatogenesis (fshr, wt1, sox9, and cldn11), although spermatogenesis was not detected in histological analysis. In contrast, all birds under 5 lux had 10 to 30 times larger testes than birds in all other groups, with a parallel strong increase in mRNA transcript levels and clear signs of spermatogenesis. Across treatments, the volume of the testes was generally a good predictor of testicular transcript levels. Overall, our findings indicate that even small changes in nocturnal light intensity can increase, or decrease, effects on the reproductive physiology of wild organisms.

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Section: Germ Cells Development Stra8mentioning

confidence: 99%

“…lhr Leydig cell gonadotropin receptor, stimulates steroid synthesis (Akazome et al, 2002;Yamamura et al, 2000) hsd3b1…”

Section: Leydig Cells Activity (Steroid Synthesis)mentioning

confidence: 99%

Dose‐response effects of light at night on the reproductive physiology of great tits (Parus major): Integrating morphological analyses with candidate gene expression

et al. 2018

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“…In general, hormones can exert their biological effects only when they specifically combine with their receptors to form hormone-receptor complexes. Progesterone (PR), androgen (AR) and estrogen (ER) receptors are located in the nucleus of GCs and TCs and bind specifically to hormones to form hormone receptor complexes, which are involved in follicular growth and development, maturation and ovulation, as well as the synthesis of steroids [ 5 , 6 , 7 , 8 ]. The genes such as steroidogenic acute regulatory protein (StAR), cytochrome P450 family 11 subfamily A member 1 (CYP11A1), 3β-hydroxysteroid dehydrogenase (3 -HSD), and cytochrome P450 family 19 subfamily A member 1 (CYP19A1) are directly involved in the synthesis of progesterone and estrogen [ 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

gga-miR-449b-5p Regulates Steroid Hormone Synthesis in Laying Hen Ovarian Granulosa Cells by Targeting the IGF2BP3 Gene

Zhang

et al. 2022

Animals

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MiRNAs have been found to be involved in the regulation of ovarian function as important post-transcriptional regulators, including regulators of follicular development, steroidogenesis, cell atresia, and even the development of ovarian cancer. In this study, we evaluated the regulatory role of gga-miR-449b-5p in follicular growth and steroid synthesis in ovarian granulosa cells (GCs) of laying hens through qRT‒PCR, ELISAs, western blotting and dual-luciferase reporter assays, which have been described in our previous study. We demonstrated that gga-miR-449b-5p was widely expressed in granulosa and theca layers of the different-sized follicles, especially in the granulosa layer. The gga-miR-449b-5p had no significant effect on the proliferation of GCs, but could significantly regulate the expression of key steroidogenesis-related genes (StAR and CYP19A1) (p < 0.01) and the secretion of P4 and E2 (p < 0.01 and p < 0.05). Further research showed that gga-miR-449b-5p could target IGF2BP3 and downregulate the mRNA and protein expression of IGF2BP3 (p < 0.05). Therefore, this study suggests that gga-miR-449b-5p is a potent regulator of the synthesis of steroid hormones in GCs by targeting the expression of IGF2BP3 and may contribute to a better understanding of the role of functional miRNAs in laying hen ovarian development.

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Expression of messenger RNAs of luteinizing hormone and follicle-stimulating hormone receptors in the granulosa layer during the ovulatory cycle of the hen

Cited by 2 publications

References 0 publications

Dose‐response effects of light at night on the reproductive physiology of great tits (Parus major): Integrating morphological analyses with candidate gene expression

Dose‐response effects of light at night on the reproductive physiology of great tits (Parus major): Integrating morphological analyses with candidate gene expression

gga-miR-449b-5p Regulates Steroid Hormone Synthesis in Laying Hen Ovarian Granulosa Cells by Targeting the IGF2BP3 Gene

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