Expression of metalloprotease insulin-degrading enzyme (insulysin) in breast and ovarian cancer tissues

Yfanti, Christina; Mengele, Karin; Weirich, Gregor; Giersig, C.; Rosner, Marsha Rich; Schmitt, Marcus

doi:10.1055/s-0028-1088826

Cited by 4 publications

(5 citation statements)

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“…IGFBP-2 has also been associated with an anti-apoptotic effect via caspase-3 [22] . These hormones are in turn degraded by insulysin [23] , whose concentration was higher in NSCLC tumor tissue. The hormone adiponectin controls lipid metabolism and insulin sensitivity, and we found adiponectin down-regulated in NSCLC tumors.…”

Section: Resultsmentioning

confidence: 99%

Protein Signature of Lung Cancer Tissues

et al. 2012

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Lung cancer remains the most common cause of cancer-related mortality. We applied a highly multiplexed proteomic technology (SOMAscan) to compare protein expression signatures of non small-cell lung cancer (NSCLC) tissues with healthy adjacent and distant tissues from surgical resections. In this first report of SOMAscan applied to tissues, we highlight 36 proteins that exhibit the largest expression differences between matched tumor and non-tumor tissues. The concentrations of twenty proteins increased and sixteen decreased in tumor tissue, thirteen of which are novel for NSCLC. NSCLC tissue biomarkers identified here overlap with a core set identified in a large serum-based NSCLC study with SOMAscan. We show that large-scale comparative analysis of protein expression can be used to develop novel histochemical probes. As expected, relative differences in protein expression are greater in tissues than in serum. The combined results from tissue and serum present the most extensive view to date of the complex changes in NSCLC protein expression and provide important implications for diagnosis and treatment.

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Section: Resultsmentioning

confidence: 99%

Protein Signature of Lung Cancer Tissues

et al. 2012

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“…We found that IDE was widely expressed in the tested tissues (kidney, lung, spleen, liver, heart, and skeletal muscle), and relatively higher expression was shown in kidney, lung, and spleen compared to heart and skeletal muscle. Previous studies reported that IDE mRNA was highly abundant in kidney and liver of rat [23], and IDE protein was extensively expressed in human tissues, including kidney, liver, lug, brain, and muscle [24]. The similar expression pattern of IDE in different species, but at different levels in different tissues, imply conservation of primary sequence and functional roles.…”

Section: Discussionmentioning

confidence: 92%

Insulin-degrading Enzyme Regulates the Proliferation and Apoptosis of Porcine Skeletal Muscle Stem Cells via Myostatin/MYOD Pathway

Wang

Guo

Zhang

et al. 2021

Preprint

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Background: Identifying the genes relevant for muscle development is pivotal to improve meat production and quality in pigs. Insulin-degrading enzyme (IDE), a thiol zinc-metalloendopeptidase, has been known to regulate the myogenic process of mouse and rat myoblast cell lines, while its myogenic role in pigs remained elusive. Therefore, the current study aimed to identify the effects of IDE on the proliferation and apoptosis of porcine skeletal muscle stem cells and underlying molecular mechanism.Results: We found in the present study that IDE was widely expressed in porcine tissues, including kidney, lung, spleen, liver, heart, and skeletal muscle. Then, to explore the effects of IDE on the proliferation and apoptosis of porcine skeletal muscle stem cells, we subjected the cells to siRNA-mediated knockdown of IDE expression, which resulted in promoted cell proliferation and reduced apoptosis. As one of key transcription factors in myogenesis, MYOD, its expression was also decreased with IDE knockdown. To further elucidate the underlying molecular mechanism, RNA sequencing was performed. Among transcripts perturbed by the IDE knockdown after, a down-regulated gene myostatin (MSTN) which is known as a negative regulator for muscle growth attracted our interest. Indeed, MSTN knockdown led to similar results as those of the IDE knockdown, with upregulation of cell cycle-related genes, downregulation of MYOD as well as apoptosis-related genes, and enhanced cell proliferation.Conclusion: Our findings suggest that IDE regulates the proliferation and apoptosis of porcine skeletal muscle stem cells through MSTN/MYOD pathway. Thus, we recruit IDE to the gene family of regulators for porcine skeletal muscle development, and propose IDE as an example of gene to prioritize in order to improve pork production.

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“…Currently, the role of IDE in controlling cell growth and proliferation is believed to be complex because IDE is considered a tumor suppressor or novel oncogene (14). Although IDE acts as a positive regulator in a variety of human tumor cell lines or tissues, it is also expressed in normal tissues (25,26). Tundo et al reported that IDE was overexpressed in tumors of the central nervous system, while silencing IDE inhibited cell proliferation and triggered the death of neuroblastoma cells (30).…”

Section: Discussionmentioning

confidence: 99%

“…Overall, our findings suggest that IDE may be involved in male reproduction by regulating Sertoli cell proliferation, which provides new information to better understand the regulatory mechanisms of swine Sertoli cells and improve the reproductive traits of male pigs. prognostic marker for cancer progression (25,26). Furthermore, reduced IDE expression sustained myoblast proliferation, but delayed myogenic differentiation in mice (27).…”

Section: Introductionmentioning

confidence: 99%