Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes

Young, David A.; Gavrilov, Svetlana; Pennington, Caroline J.; Nuttall, Robert K.; Edwards, Dylan R.; Kitsis, Richard N.; Clark, Ian M.

doi:10.1016/j.bbrc.2004.07.178

Cited by 42 publications

(32 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, quantification of certain MMPs and their inhibitors in blood has been correlated with the degree of atherogenesis in humans (120). The MMPs are also involved in several non-atherosclerotic processes within the heart (121)(122)(123)(124)(125)(126). Most importantly, as previously mentioned, disruption of the IEL as a result of the adventitia/media infiltrated by monocytes, which release MMPs mostly at areas of neovascularization.…”

Section: Plaque Rupturementioning

confidence: 99%

Atherothrombosis and High-Risk Plaque

Fuster

Moreno

Fayad

et al. 2005

Journal of the American College of Cardiology

687

419

View full text Add to dashboard Cite

Atherothrombosis is a complex disease in which cholesterol deposition, inflammation, and thrombus formation play a major role. Rupture of high-risk, vulnerable plaques is responsible for coronary thrombosis, the main cause of unstable angina, acute myocardial infarction, and sudden cardiac death. In addition to rupture, plaque erosion may also lead to occlusive thrombosis and acute coronary events. Atherothrombosis can be evaluated according to histologic criteria, most commonly categorized by the American Heart Association (AHA) classification. However, this classification does not include the thin cap fibroatheroma, the most common form of high-risk, vulnerable plaque. Furthermore, the AHA classification does not include plaque erosion. As a result, new classifications have emerged and are reviewed in this article. The disease is asymptomatic during a long period and dramatically changes its course when complicated by thrombosis. This is summarized in five phases, from early lesions to plaque rupture, followed by plaque healing and fibrocalcification. For the early phases, the role of endothelial dysfunction, cholesterol transport, high-density lipoprotein, and proteoglycans are discussed. Furthermore, the innate and adaptive immune response to autoantigens, the Toll-like receptors, and the mechanisms of calcification are carefully analyzed. For the advanced phases, the role of eccentric remodeling, vasa vasorum neovascularization, and mechanisms of plaque rupture are systematically evaluated. In the final thrombosis section, focal and circulating tissue factor associated with apoptotic macrophages and circulatory monocytes is examined, closing the link between inflammation, plaque rupture, and blood thrombogenicity.

show abstract

Section: Plaque Rupturementioning

confidence: 99%

Atherothrombosis and High-Risk Plaque

Fuster

Moreno

Fayad

et al. 2005

Journal of the American College of Cardiology

687

419

View full text Add to dashboard Cite

show abstract

“…Although DMSO is regarded as relatively nontoxic, the clinical use of frozen/thawed cells treated with DMSO can cause many adverse effects and toxic reactions [7][8][9][10][11][12][13][14]. It has also been reported that DMSO is not only cytotoxic but it also induces differentiation of stem cells to cardiac or neuronal like cells when added to the cell culture medium [15,16]. It is, therefore, imperative to reduce the toxicity by the removal of DMSO prior to clinical use.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Methylcellulose and Dimethyl Sulfoxide as the Cryoprotectants in a Serum-Free Freezing Media for Cryopreservation of Adipose-Derived Adult Stem Cells

Thirumala

Gimble

Devireddy

2010

Stem Cells and Development

102

View full text Add to dashboard Cite

Developing effective techniques for the cryopreservation of human adipose-derived adult stem cells (ASCs) could increase the usefulness of these cells in tissue engineering and regenerative medicine. To this end, we investigated the post-freeze/thaw viability and apoptotic behavior of Passage 1 (P1) adult stem cells (ASCs) in 11 different media: (i) the traditional media containing Dulbecco's modifi ed Eagle's medium (DMEM) with 80% fetal calf serum (FCS) and 10% dimethyl sulfoxide (DMSO), (ii) DMEM with 80% human serum (HS) and 10% DMSO, (iii) DMEM with 1% methyl cellulose (MC) and 10% of either HS or FCS or DMSO, and (iv) DMEM with 0%, 2%, 4%, 6%, 8%, or 10% DMSO. Approximately 1 mL (10 6 cells/mL) of P1 ASCs were frozen overnight in a −80°C freezer and stored in liquid nitrogen for 2 weeks before being rapidly thawed in a 37°C water bath (1-2 min of agitation), resuspended in culture media, and seeded in separate wells of a 6-well plate for a 24-h incubation period at 37°C. After 24 h, the thawed samples were analyzed by bright-fi eld microscopy and fl ow cytometry. The results suggest that the absence of DMSO (and the presence of MC) signifi cantly increases the fraction of apoptotic and/or necrotic ASCs. However, the percentage of viable cells obtained with 2% DMSO and DMEM was comparable with that obtained in freezing media with 10% DMSO and 80% serum (HS or FCS), that is, ~84% ± 5% and ~84% ± 8%, respectively. Adipogenic and osteogenic differentiation behavior of the frozen thawed cells was also assessed using histochemical staining. Our results suggest that post-thaw ASC viability, adipogenic and osteogenic differentiability can be maintained even when they are frozen in the absence of serum but with a minimal concentration of 2% DMSO in DMEM.

show abstract

“…[42][43][44][45][46][47][48][49] It has also been reported that DMSO is not only cytotoxic but it also induces differentiation of stem cells to cardiac or neuronal like cells when added to the cell culture medium. 50,51 Therefore, it is of vital necessity to reduce the toxicity by the removal of DMSO prior to clinical use. However, the total removal of DMSO from the frozenthawed cells is complex and time consuming.…”

Section: Clinical Banking Of Adipose Derived Adult Stem Cells (Ascs)mentioning

confidence: 99%