Expression of metallothionein in ameloblastoma. A regulatory molecule?

Ribeiro, André Luís Ribeiro; Nobre, Rafaela Moutinho; Rocha, Gabriela Cristina Marçal Avertano; Lobato, Isabella Haber de Souza; Alves-Júnior, Sérgio de Melo; Jaeger, Ruy Gastaldoni; Pinheiro, João de Jesus Viana

doi:10.1111/j.1600-0714.2011.01025.x

Cited by 12 publications

(19 citation statements)

References 13 publications

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“…The reason for immunoexpression of MT in ameloblastomas was thought to be that MT acts as a zinc reservoir for important proteases, such as matrix metalloproteases (MMPs) which help in the invasion of odontogenic neoplasms [1].…”

Section: Discussionmentioning

confidence: 99%

“…MT belongs to a family of low molecular weight of about 6-7 kDa, non-enzymatic, cysteine rich proteins with high affinity for metal ions [1]. They consist of a polypeptide chain of 61-68 amino acids, of which around 30 % are cysteine residues.…”

Section: Introductionmentioning

confidence: 99%

“…MTs make up a superfamily of proteins, with four isoforms identified as MT-1, MT-2, MT-3 and MT-4, encoded by a family of genes located on chromosome 16q13 [1][2][3][4]. MTs 1 and 2 are present in almost all the organs while MT-3 is found mainly in the brain and MT-4 in some stratified epithelia [1].…”

Section: Introductionmentioning

confidence: 99%

“…MTs 1 and 2 are present in almost all the organs while MT-3 is found mainly in the brain and MT-4 in some stratified epithelia [1]. Since MT is present in most tissues and cell types in small amounts, it is generally considered as a ''housekeeping'' protein [5].…”

Section: Introductionmentioning

confidence: 99%

“…Various studies have demonstrated increased expression of MT in various human tumours such as larynx, pharynx, lung, kidney, chest, skin, ovary, prostate, bladder and pancreatic carcinomas compared to normal tissues [1]. However, very few studies have shown expression of MT in oral squamous cell carcinomas (OSCC).…”

Section: Introductionmentioning

confidence: 99%

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Immunoexpression of Metallothionein in Oral Squamous Cell Carcinoma

Dumpala

Guttikonda

2015

J. Maxillofac. Oral Surg.

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Background and Objectives Metallothionein (MT) is a family of ubiquitous low molecular weight (7 kDa), intracellular (cytoplasmic/nuclear), cysteine rich proteins with high affinity for heavy metals, present in both normal cells and neoplastic cells. Increased expression of MT has been reported to be associated with poor prognosis in various tumours. The objectives of the present study were to compare the expression of MT in normal subjects and in patients with oral squamous cell carcinoma (OSCC), to correlate the expression of MT with respect to clinical staging of OSCC and to evaluate the expression of MT with respect to different histopathological grades of OSCC. Methods Thirty cases of OSCC were staged clinically and graded histopathologically. Immunohistochemical method was used to detect the expression of MT in OSCC. The scores obtained were documented and compared statistically. Results MT immunoreactivity was noticed in all cases of OSCC. A statistically significant difference was observed in immunoreactivity of MT between the normal and OSCC, and in different histopathological grades of OSCC (p = 0.00001*). However, no statistical significance was found in a number of immunopositive cells in different clinical stages of OSCC (p = 0.7573). Conclusion The MT immunoexpression increased from low grade to high grade OSCC. Hence, increased expression of MT may be related to poor prognosis in patients with OSCC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunoexpression of Metallothionein in Oral Squamous Cell Carcinoma

Dumpala

Guttikonda

2015

J. Maxillofac. Oral Surg.

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Uncommon Tumors of the Oral Cavity and Adjacent Structures

Bar‐Ad¹,

Tuluc²,

Cognetti³

et al. 2012

Textbook of Uncommon Cancer

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Role of HIF-1α and CASPASE-3 in cystogenesis of odontogenic cysts and tumors

et al. 2017

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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 Objectives: Odontogenic cysts and tumors are the most relevant lesions that affect the gnathic bones. These lesions have in common the formation of cystic areas and this common feature may suggest involvement of similar mechanisms. The hypoxia inducible factor 1 alpha (HIFa responsive protein to hypoxia and caspase-3, an irreversible apoptosis marker, may contribute to cyst formation. Thus, this study aimed to investigate the immunoexpression of these proteins in odontogenic cysts and tumors. Material and methods: Twenty cases of ameloblastoma, keratocystic odontogenic tumor (KOT) (n=20), radicular cyst (RC) (n=18), dentigerous cyst (DC) (n=11), calcifying cystic odontogenic tumor (n=8) and dental follicle (DF) (n=10) were used to investigate HIF-and caspase-3 expression in sequential serial cuts by immunohistochemistry. Results: HIF-was overexpressed in RC, DC and ameloblastoma when compared with DF. The basal and sometimes the lower suprabasal layer showed no or very low expression in DC, KOT and ameloblastoma, the last also showing strong expression in solid epithelial areas and initial cystic formation regions. Caspase-3 was found to be overexpressed in all lesions, with the highest expression in odontogenic cysts compared to tumors. HIF-and caspase-3 were localized in similar areas of the same lesions, especially in the epithelium surrounding cystic formations. Conclusions: This study showed distinct immunoexpression of HIF-and caspase-3 in odontogenic cyst and tumors, with higher expression observed in odontogenic cysts. Clinical relevance: These finding suggesting a possible correlation between hypoxia, apoptosis and cystogenesis, leading to understand the mechanisms responsible to cystic formation in odontogenic lesions.

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Expression of metallothionein in ameloblastoma. A regulatory molecule?

Abstract: This protein may have an impact on ameloblastoma behavior. Metallothionein would act as a zinc reservoir for important proteases related to ameloblastoma biology, such as MMPs. This protein could also display pro-mitotic and anti-apoptotic features in the tumor.

Cited by 12 publications

References 13 publications

Immunoexpression of Metallothionein in Oral Squamous Cell Carcinoma

Immunoexpression of Metallothionein in Oral Squamous Cell Carcinoma

Uncommon Tumors of the Oral Cavity and Adjacent Structures

Role of HIF-1α and CASPASE-3 in cystogenesis of odontogenic cysts and tumors

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