Expression of MHC products and leucocyte differentiation antigens in gynaecological neoplasms: An immunohistological analysis of the tumour cells and infiltrating leucocytes

Ferguson, Anna; Moore, Michael G.; Fox, Howard S.

doi:10.1038/bjc.1985.227

Cited by 65 publications

(39 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…conditions known to be associated with higher proliferation rate, suggested to us a possible relationship between the increased mitotic rate and the induction of HLA-DR molecules on human mammary gland epithelial cells. In this context, it is interesting that the expression of HLA-DR antigens in endometrial epithelium was reported to parallel the rise and fall of DNA synthesis and mitoses (Tabibzadeh et al, 1986) though this correlation was not found in a previous study by Ferguson et al (1985). To test the hypothetical association of the HLA-DR expression with proliferation, we deployed the collagen gel culture of breast epithelial organoids pulsed with tritiated thymidine followed by the combination of immunohistochemical staining for HLA-DR and autoradiography.…”

Section: Discussionmentioning

confidence: 99%

HLA-DR antigens on differentiating human mammary gland epithelium and breast tumours

Bártek

Petřek²,

Vojtěšek³

et al. 1987

Br J Cancer

View full text Add to dashboard Cite

Summary The staining pattern of a monoclonal antibody directed to the monomorphic determinant of HLA-DR antigens was examined on sections of human mammary gland tissues at various stages of differentiation as well as on 50 benign and 72 malignant breast lesions. Normal resting breast epithelium lacked HLA-DR, whereas late-pregnant and lactating epithelia expressed high levels of HLA-DR antigens, followed by a decline in the post-weaning regression period. Most benign breast lesions revealed heterogeneous staining ranging from very few up to 20-25% positive epithelial cells. Greater variability was observed among carcinomas, where a small group (-7%) of cases showing 40-95% positive tumour cells was found, in addition to negative tumours and those with the minority of HLA-DR expressing carcinoma cells. The density of the leukocytic infiltrate was higher in carcinomas than in either normal breast tissue or benign lesions, the HLA-DR phenotype of the mononuclear infiltrating cells lacking any obvious correlation with the HLA-DR status of the epithelial component. Immunoblotting analyses of whole-tissue lysates separated by SDS-PAGE confirmed the immunohistochemical data and demonstrated the reactivity with only one protein band predicted for HLA-DR a-chain. The combination of immunohistochemistry and autoradiography on sections of human reduction mammoplasty organoids cultured in collagen gels and labelled with tritiated thymidine revealed a lack of HLA-DR expression on proliferating breast epithelial cells suggesting factors other than cell kinetics must be responsible for induction of HLA-DR antigens seen in pregnant and lactating breast epithelium and some tumours.

show abstract

Section: Discussionmentioning

confidence: 99%

HLA-DR antigens on differentiating human mammary gland epithelium and breast tumours

Bártek

Petřek²,

Vojtěšek³

et al. 1987

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…This could explain the favourable prognosis of tumours expressing MHC-II antigens (Esteban et al, 1990;Gutierrez et al, 1987). However, upregulation of MHC-II expression in melanoma is reported to be associated with a shorter disease-free survival (Ruiter et al, 1986) (Ferguson et al, 1985;Glew et al, 1992) and in CIN lesions (Warhol et al, 1989 Aberrant MHC-I expression could be observed in HPV negative lesions, as well as lesions containing high-risk HPVs (i.e. HPV 16, 18 and 31) and HPV types 6 or X, as determined by PCR.…”

Section: Mhc Class I and Ii Expressionmentioning

confidence: 99%

Analysis of MHC class I and II expression in relation to presence of HPV genotypes in premalignant and malignant cervical lesions

Cromme

Meijer²,

Snijders³

et al. 1993

Br J Cancer

110

View full text Add to dashboard Cite

Summary Cervical intraepithelial neoplasia (CIN) grades I to III lesions (n = 94) and squamous cell carcinomas of the uterine cervix (n = 27) were analysed for MHC class I and II expression and presence of HPV genotypes.MHC class I and II expression was studied by immunohistochemistry and HPV typing was performed by general primer-and type-specific primer mediated PCR (GP/TS PCR). Both A central role in the antigen-specific immune response is played by the major histocompatibility complex (MHC), which are cell surface proteins that act as restricting elements in the recognition of antigen by T-cells. MHC class I (MHC-I) present endogenous antigen to cytotoxic T-lymphocytes (CTLs). Low levels or lack of MHC-I surface expression can consequently render aberrant cells non-immunogenic to CTLs, and may provide a way for cells to escape immune surveillance. MHC-1 alterations have been described in human cancer of different sites of the body (see review RuizCabello et al., 1991).Generally, MHC class II (MHC-II) surface expression is restricted to specialised antigen presenting cells (APCs), that present mainly opsonised exogenous antigen to T-helper cells. Recognition leads to activated T-cells, which can stimulate B-cell, CTL proliferation and MHC non-restricted killing by natural killer (NK) cells or activated macrophages. Occasionally, other cells like neoplastic epithelial cells have been described to express MHC-II, which could assist in the onset of the cellular immune response (Ostrand-Rosenberg et al., 1991).Infections with specific human papillomavirus (HPV) types are strongly associated with the development of cervical cancer, with HPV types 16 and 18 as the most predominant types (Zur Hausen, 1989 (Connor & Stern, 1990;Glew et al., 1992), suggesting that changes in the presentation of viral tumour antigens to the cellular immune system can occur. However, to get insight whether altered MHC-I and -II expression is related to the development of cervical cancer from its premalignant lesions, it is necessary to study dysplastic cervical lesions (CIN) for the MHC-I and -II status.Tumour virus based mechanisms have been described that specifically influence MHC-I cell surface expression (Signas et al., 1982;Schrier et al., 1983). Similar mechanisms could exist for HPV affecting antigen presentation of the infected cells. However, little is known about MHC alterations in CIN lesions in relation to the presence of different HPV genotypes.Therefore in this study expression of MHC-I and -II was investigated in CIN lesions of different grades and cervical carcinomas. HPV typing was carried out by a combined general primer-mediated (GP-) and type-specific (TS-) polymerase chain reaction (PCR) strategy (van den Brule et al., 1991;Walboomers et al., 1992). In addition, HPV RNA in situ hybridisation (RISH) was applied to some HPV 16 PCR positive lesions, in order to localise cells containing transcriptionally active HPV 16 in relation to altered MHC expression. The results indicate that MHC-I and -II alterations are also...

show abstract

Section: Introductionmentioning

confidence: 99%

“…The majority of TILs in endometrial carcinomas express the CD8 ϩ suppressor/ cytotoxic phenotype, and minor subsets express B-lymphocyte and macrophage markers (2,11). Natural killer cells are virtually absent in endometrial tumors (11). Activated CTLs expressing TIA-1 and/or granzyme B cytotoxic granules have been demonstrated in certain human neoplasms, such as EBV-associated gastric cancer, medullary carcinoma of the breast, germ cell tumors of the testis, and carcinomas of the colon, cervix, esophagus, and kidney (12)(13)(14)(15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Intratumoral CD8+ T Lymphocytes as a Prognostic Factor of Survival in Endometrial Carcinoma

Kondratiev¹,

Sabo²,

Yakirevich³

et al. 2004

Clinical Cancer Research

195

141

View full text Add to dashboard Cite

Results: Patients with >10 CD8 ؉ T lymphocytes/highpower field within the tumor epithelium at the invasive border displayed improved overall survival compared with patients with fewer intraepithelial CD8 ؉ T lymphocytes (87 and 50%, respectively; P ‫؍‬ 0.027). Multivariate analysis revealed that stage, vascular invasion, grade, and the number of intraepithelial CD8؉ T lymphocytes at the invasive border were the only independent predictors of survival (P < 0.0001, P ‫؍‬ 0.001, P ‫؍‬ 0.011, and P ‫؍‬ 0.025, respectively). Granzyme B ؉ cytoplasmatic granules were detected in a high proportion of CTLs, confirming their activated cytotoxic phenotype.Conclusions: Our study demonstrates for the first time that increased numbers of CTLs at the invasive border may be a reliable independent prognostic factor of survival in patients with endometrial carcinoma.

show abstract

Expression of MHC products and leucocyte differentiation antigens in gynaecological neoplasms: An immunohistological analysis of the tumour cells and infiltrating leucocytes

Cited by 65 publications

References 46 publications

HLA-DR antigens on differentiating human mammary gland epithelium and breast tumours

HLA-DR antigens on differentiating human mammary gland epithelium and breast tumours

Analysis of MHC class I and II expression in relation to presence of HPV genotypes in premalignant and malignant cervical lesions

Intratumoral CD8+ T Lymphocytes as a Prognostic Factor of Survival in Endometrial Carcinoma

Contact Info

Product

Resources

About