Expression of microRNA-155 and human telomerase reverse transcriptase in patients with bladder cancer

Abstract

Bladder cancer (BC) is the most common malignant tumor in the urinary system, with a high incidence and recurrence rate. microRNA-155 (miR-155), and human telomerase reverse transcriptase (hTERT) had a role in BC pathogenesis. In our study, we investigated the usability of miR-155, and hTERT in BC detection, muscle invasion presence, and grades of non-muscle invasive BC (NMIBC). miR-155 and hTERT were determined in urine sediment of 30 BC patients and 15 control patients with normal urologic workup by using realtime polymerase chain reaction (RT-qPCR) technique. A receiver operating characteristic (ROC) was plotted to choose the best cutoff point. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated for each marker. BC patients had lower expression of miR-155 (p= 0.005) and higher expression of hTERT (p ˂ 0.001) than control patients. Both markers had higher sensitivity than cytology for BC diagnosis (p˂ 0.001). Except for miR-155, hTERT could differentiate between NMIBC and MIBC (p˂ 0.001). miR-155 and hTERT showed no significant difference between different grades of NMIBC with p= 0.73 and p=0.07, respectively. Our results indicated that these urinary markers have the potential to be developed as non-invasive markers for BC.

Epigallocatechin-3-gallate and cancer: focus on the role of microRNAs

“…On the other hand, cancer cells maintain their immortality by maintaining telomeres through the upregulation of telomerase. MiRNAs that are not functioning properly can lead to an increase in telomerase activity in tumors [ 71 ]. A number of miRNA have been found to be involved in epithelial-mesenchymal transition (EMT), which is a fundamental element of the process of invasion and metastasis of tumors [ 72 ].…”

Palladium(II) Picoline Thiourea Complex as Homogenous Catalyst in Heck Cross-Coupling Reaction in the Formation of C=C Bond

“…Due to these existing problems, researchers have actively carrying out attempts on discovery of phosphine-free ligands such as N-heterocyclic carbenes [10][11] and sulfur-containing ligands [12] to be applied in metal-catalyzed synthetic reactions in order to achieve good stability and substrate tolerance which allow the coupling reaction to be conducted under mild condition or at ambient environment. Recently, thiourea system has attracted considerable attention to be used in catalysis system [13][14][15] due to its unique properties such as able to coordinate with various transition metal ions as monodentate or bidentate ligands [16][17], great tunability by varying substituents on the nitrogen and stable in moisture. By considering their physical and chemical properties, thiourea derivatives have been introduced to be used as ligand in palladium complexes in order to replace the functionality of phosphine in catalysis system.…”