Expression of microRNA‐218 in human pancreatic ductal adenocarcinoma and its correlation with tumor progression and patient survival

Zhu, Ziman; Xu, Yuefang; Du, Jundong; Tan, Jun; Jiao, Huabao

doi:10.1002/jso.23475

Cited by 27 publications

(26 citation statements)

References 43 publications

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“…The abnormal expression of miRNAs in PDAC was previously reported (24)(25)(26); however, few studies have investigated the role of specific miRNAs in carcinogenesis and progression of PDAC. The present study has presented the first data characterizing the expression, biological functions and molecular mechanisms underlying miR-216b in PDAC.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑216b reduces growth, migration and invasion of pancreatic ductal adenocarcinoma cells by directly targeting ρ‑associated coiled‑coil containing protein kinase 1

et al. 2018

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Abstract. Developments in cancer therapy have greatly improved the survival time for patients with pancreatic ductal adenocarcinoma (PDAC); however, the prognosis of patients with PDAC remains poor. Understanding the expression patterns and functions of microRNAs may provide strategies for the diagnosis and treatment of patients with PDAC. The present study aimed to explore the expression and functions of microRNA-216b (miR-216b) in PDAC. The expression of miR-216b in PDAC tissues and cell lines was quantified with reverse transcription-quantitative polymerase chain reaction. An miR-216b mimic was introduced into PDAC cells to induce the effects of miR-21b overexpression. The effects of miR-216b overexpression on growth, migration and invasion of PDAC cells were evaluated by cell proliferation assay, migration and invasion assays, respectively. The molecular mechanism underlying the suppressive effects of miR-216b on PDAC was also examined; a direct target gene of miR-216b, ρ-associated coiled-coil containing protein kinase 1 (ROCK1), was downregulated by ROCK1 short interfering RNA to investigate the effects on growth, migration and invasion of PDAC cells. The present study revealed that miR-216b was significantly downregulated in PDAC tissues and cell lines. Overexpression of miR-216b inhibited growth, migration and invasion of PDAC cells in vitro. ROCK1 was identified as a direct target gene of miR-216b in pancreatic cancer and the downregulation of ROCK1 resembled the effects of miR-216b overexpression in PDAC cells. Taken together, miR-216b acted as a tumor suppressor in PDAC and may represent a novel therapeutic target in PDAC.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‑216b reduces growth, migration and invasion of pancreatic ductal adenocarcinoma cells by directly targeting ρ‑associated coiled‑coil containing protein kinase 1

et al. 2018

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“…Title and abstract assessment identified 37 manuscripts appropriate for evaluation of prognostic miRNA biomarkers in PDAC and full text articles were obtained for these. On careful review of study methodologies, 19 reports were excluded (Additional file 1: Table S1), of which 13 were studies focusing on a miRNA not investigated by a second study and therefore a pooled analysis would not be possible [19][20][21][22][23][24][25][26][27][28][29][30][31]. The details for the other 6 excluded studies can be seen in Additional file 2: Supplementary Results.…”

Section: Excluded Studiesmentioning

confidence: 99%

microRNAs with prognostic significance in pancreatic ductal adenocarcinoma: A meta-analysis

Frampton¹,

Krell²,

Jamieson³

et al. 2015

European Journal of Cancer

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“…In HCC, the low expression of miR-218 was reported to confer a poor 5-year survival in HCC patients and miR-218 may serve as a prognostic biomarker and induce apoptosis and growth arrest by downregulating BMI-1 in HCC (Tu et al, 2014). Likewise, reduced miR-218 in pancreatic ductal adenocarcinoma tissues was correlated with tumor progression, and might be an independent poor prognostic factor for patients (Zhu et al, 2014). Meanwhile, this miRNA inhibits cell invasion and migration of pancreatic cancer via regulating ROBO1 (He et al, 2014).…”

Section: Discussionmentioning

confidence: 98%

Expression of microRNA-218 and its Clinicopathological and Prognostic Significance in Human Glioma Cases

Cheng¹,

Wang²,

Hu³

2015

Asian Pacific Journal of Cancer Prevention

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Background: MicroRNAs are a class of noncoding RNAs which regulate multiple cellular processes during tumor development. The purpose of this report is to investigate the clinicopathological and prognostic significance of miR-218 in human gliomas. Materials and Methods: Quantitative RT-PCR (qRT-PCR) was conducted to detect the expression of miR-218 in primary normal human astrocytes, three glioma cell lines and 98 paired glioma and adjacent normal brain tissues.Associations of miR-218 with clinicopathological variables of glioma patients were statistically analyzed. Finally, a survival analysis was performed using the Kaplan-Meier method and Cox's proportional hazards model. Results: The expression level of miR-218 in primary normal human astrocytes was significantly higher than that in glioma cell lines (p<0.01). Also, the expression level of miR-218 in glioma tissues was significantly downregulated in comparison with that in the adjacent normal brain tissues (p<0.001). Statistical analyses demonstrated that low miR-218 expression was closely associated with advanced WHO grade (p=0.002) and low Karnofsky performance score (p=0.010) of glioma patients. Kaplan-Meier analysis with the log-rank test showed that patients with low-miR-218 expression had poorer disease-free survival and overall survival (p=0.0045 and 0.0124, respectively). Multivariate analysis revealed that miR-218 expression was independently associated with the disease-free survival (p=0.009) and overall survival (p=0.004) of glioma patients. Conclusions: Our results indicate that miR-218 is downregulated in gliomas and that its status might be a potential valuable biomarker for glioma patients.

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Expression of microRNA‐218 in human pancreatic ductal adenocarcinoma and its correlation with tumor progression and patient survival

Abstract: Reduced miR-218 in PDAC tissues was correlated with tumor progression, and might be an independent poor prognostic factor for patients.

Cited by 27 publications

References 43 publications

MicroRNA‑216b reduces growth, migration and invasion of pancreatic ductal adenocarcinoma cells by directly targeting ρ‑associated coiled‑coil containing protein kinase 1

MicroRNA‑216b reduces growth, migration and invasion of pancreatic ductal adenocarcinoma cells by directly targeting ρ‑associated coiled‑coil containing protein kinase 1

microRNAs with prognostic significance in pancreatic ductal adenocarcinoma: A meta-analysis

Expression of microRNA-218 and its Clinicopathological and Prognostic Significance in Human Glioma Cases

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