Expression of miR-625 and Fas in cervical vertebral cartilage endplate

Abstract

Abstract. The aim of the present study was to assess miR-625 and Fas expression in normal and degenerative cervical cartilage endplate (CEP) tissues. Following biof-informatics analysis, the Fas gene was predicted to be one of the targets of miR-625. Quantitative PCR (qPCR) and western blotting were used to detect miR-625 and Fas expression in normal and degenerative CEP. A luciferase reporter assay was used to identify whether miR-625 could directly target the 3' untranslated region (3'-UTR) of Fas. Lentiviral overexpression and/or inhibition vectors of miR-625 (pre-miR-625)/antigomiR-625 were constructed to determine whether overexpression or inhibition of miR-625 could affect Fas and B-cell lymphoma 2 (Bcl-2) expression in cartilaginous endplate cells (CECs) and tissues. qPCR analysis demonstrated that miR-625 expression in degenerative CEP was significantly lower than in normal CEP tissue, while the production of Fas in degenerated CEP was significantly higher. Results from western blotting also showed a significant increase in Fas expression in degenerative CEP. miR-625 can bind directly to the 3'-UTR of the Fas gene. However, this inhibition was attenuated by a target mutation in the miR-625-binding site of the 3'-UTR of Fas mRNA. In addition, following transfection of CECs with pre-miR-625 and antigomiR-625, expression of Fas significantly decreased and increased, respectively, and Bcl-2 expression was upregulated and downregulated, respectively. Upregulation of miR-625 can inhibit Fas expression and further affect Bcl-2 expression in CEP degeneration, suggesting that miR-625-mediated inhibition of the Fas gene is important in cervical degeneration.

Wang Yangming in Beijing, 1510–1512: “If I Do Not Awaken Others, Who Will Do So?”

“…Ultimately, that defined the Way of the sages. 138 Zhan would surely have assented to these propositions, except perhaps Wang's liberal reading of the value of Buddhist insight into nature and destiny. On the one hand, Wang's confident claim that "Ganquan's 甘泉 [i.e.…”

“…But if he really does, I haven't seen it!" 140 In a parting preface written for a colleague about one month earlier, Zhan had asserted that if a person knows what makes a Ru (scholar) a Ru, he will understand why a Buddhist is different. Practitioners of both traditions experience tranquility and motion.…”

A novel circRNA–miRNA–mRNA network reveals hsa-circ-0040039 as a biomarker for intervertebral disc degeneration

“…IDD has been shown to be closely associated with apoptosis of nucleus pulposus cells. 49 The pathology of IDD is influenced by decreases in the composition and synthesis of the extracellular matrix because of a reduction in the activity of the intervertebral disc cells due to increased cell apoptosis. 50 The PPI showed that RAB1A, RAB1B, and MCFD2 played important roles in disc degeneration.…”

Role of microRNAs in intervertebral disc degeneration (Review)

“…However, it was demonstrated that upregulation of miR-34a expression in human degenerated chondrocytes caused Fas-mediated CEP chondrocyte apoptosis (110). Similar to miR-34a, downregulation of miR-625 also caused Fas-mediated cervical CEP chondrocyte apoptosis (111). These findings may indicate that the effects of miRNAs on chondrocyte apoptosis in the CEP may be mediated by Fas.…”

“…The occurrence of IDD may be associated with CEP degeneration, dysfunction, and calcification (108,109). Some miRNAs (110)(111)(112)(113)(114) have been found to be involved in the changes of the CEP in the progress of IDD (Table V).…”