Expression of miRNAs in non-small-cell lung carcinomas and their association with clinicopathological features

Abstract

Lung cancer is recognized as a leading cause of cancer-related deaths worldwide. Over the past several years, evidence emerged that microRNAs (miRNAs), a class of small non-coding RNA molecules regulating gene expression at posttranscriptional level, play an important role in cell functioning, as well as in human diseases. Here, we analyzed expression of miR-15a/16, miR-21, miR-34a, miR-126, miR-128, and miR-210 at transcriptional level in 30 non-small-cell lung carcinoma (NSCLC) tumor tissues compared to the matched adjacent normal tissues and their correlation with clinicopathological features of the patients. Samples were collected from the NSCLC patients undergoing surgery before radiotherapeutic or chemotherapeutic treatment. Expression levels of miRNAs were assessed by TaqMan RT-PCR assay. The data obtained in this study were processed using REST 2009 and SPSS statistical software. The graphs were designed by GraphPad prism 5.0. In tumor samples, we found downregulation of miR-15a/16 (50/83.3%), miR-34a (83.3%), miR-126 (70%), and miR-128 (63.3%). At the same time, miR-21 and miR-210 were upregulated by 53.3 and 66.6% in cancer tissue versus matched adjacent normal tissues, respectively. No significant correlation was found between the expression levels of miR-15a/16, miR-21, miR-34a, miR-126, miR-128, and miR-210 and lymph node, tumor size, sex, and smoking. However, the study demonstrated a correlation between a change in expression of miR-15, miR-16, miR-34a, miR-126, and miR-210 compared to normal tissues and TNM staging (P < 0.05). Furthermore, miR-126 expression level was different in adenocarcinomas and squamous cell carcinoma (SCC) subtype (P < 0.1). Detailed analysis revealed significant change in expression of miR-15a/16, miR-34a, miR-126, and miR-210 in NSCLC tumor samples indicating involvement of these miRNAs in lung cancer pathogenesis. miR-210 demonstrated the most consistent increase in tumor tissues between different patients, suggesting its potential significance for NSCLC.

Physical training improves visceral adipose tissue health by remodelling extracellular matrix in rats with estrogen absence: a gene expression analysis

“…Statistical significance was accepted at a value of P < 0.05. The relative gene analyses of the study groups were performed using the REST 2009 (Relative Expression Software Tool program Qiagen â [30]), in accordance with the literature (Leduc et al 2011;Salis et al 2014;Tafsiri et al 2015). The statistical significance was accepted at a value of P < 0.05.…”

MicroRNA-29a functions as a potential tumor suppressor through directly targeting CDC42 in non-small cell lung cancer

“…miRNAs are a class of short (19)(20)(21)(22)(23)(24) nucleotides in length) endogenous non-protein-coding single-stranded RNAs, which regulate ~1/3 of mRNAs in the human genome (18). miRNAs regulate mRNA expression by binding the 3' untranslated regions (3'UTRs) of target mRNAs and inducing either translational repression or transcript degradation (19).…”

“…It has been demonstrated that miRNAs contribute to various physiological and pathological processes, including cell proliferation, differentiation, morphogenesis, cell cycle regulation, apoptosis, migration and invasion (20). miRNAs are classified as tumor-suppressive miRNAs and oncogenic miRNAs (21). Tumor suppressive miRNAs are downregulated in cancer and inhibit carcinogenesis and progression, whereas oncogenic miRNAs are upregulated in cancer, and enhance the initiation and development of cancer (22)(23)(24).…”

MiR‐138 inhibits cell proliferation and reverses epithelial‐mesenchymal transition in non‐small cell lung cancer cells by targeting GIT1 and SEMA4C

“…Zhang et al and Ye et al showed that miR-138 could inhibit NSCLC cell growth and tumour growth in nude mice by suppressing the expression of its target genes the enhancer of zeste homolog 2 (EZH2) and 3-phosphoinositide-dependent protein kinase-1 (PDK1) [16,19]. In general, however, one miRNA has numerous target genes, and a miRNA may be multifunctional, which means that miR-138 may inhibit NSCLC cell growth by targeting some other genes associated with the EMT of NSCLC [5,7,17,21,22].…”