Expression of MMP-1, -2, and -8 in longissimus dorsi muscle and their relationship with meat quality traits in cattle

Abstract

ABSTRACT. The extracellular matrix (ECM) is the major macromolecule in skeletal muscle, which affects meat quality greatly. The remodeling of the ECM is mainly regulated by matrix metalloproteinases (MMPs). The expression patterns of MMP-1, -2, and -8 in longissimus dorsi muscle were explored using quantitative real-time polymerase chain reaction. The results show that the expression of MMP-1, -2, and -8 decreased significantly from 135 days of pregnancy to postnatal 30 months. While the expression of MMP-1, -2, and -8 showed no significant relationships with intramuscular fat contents, MMP-1 and -2 showed significant negative correlations with the shearing force of the longissimus dorsi muscle in cattle. The expression of MMP-1 also showed a significant negative correlation with cooking loss and a positive correlation with water holding capacity. The expression levels of MMP-1 and -2 were usually higher in fat than in skeletal muscle tissue. The expression of MMP-8 was significantly higher in the mammary fat pad and the longissimus dorsi muscle than in all other tissues. This study indicates that the remodeling of the ECM has important effects both on the development of postnatal skeletal muscle and on meat quality.

Programmed Cell Deaths and Potential Crosstalk With Blood–Brain Barrier Dysfunction After Hemorrhagic Stroke

“…These studies may provide emerging therapeutic targets for ICH . It should be noted that MMPs, which are primarily released by neutrophils, are also thoroughly studied potent proteinases and play a critical role in the increase of endothelial permeability via disruption of TJs, ECM degradation, and increased transcytosis of the endothelium (Abilleira et al, 2003;Qi et al, 2016;. Recently, MMP-9 was also found to mediate endothelial permeability through the activity of von Willebrand factor and the initial recruitment of inflammatory leukocytes (Askenase and Sansing, 2016).…”

Transcriptome analysis reveals a molecular understanding of nicotinamide and butyrate sodium on meat quality of broilers under high stocking density

“…The remodelling of ECM is mainly regulated by matrix metalloproteinases. The expression of matrix metalloproteinase-1 is negatively correlated with cooking loss and positively correlated with hydraulic performance [51]. Collagen is an abundant connective tissue protein that is an important factor in the tenderness and texture of the meat and is well resistant to physical damage during cooking [52].…”

Transcriptome analysis reveals a molecular understanding of nicotinamide and butyrate sodium on meat quality of broilers under high stocking density

“…The remodelling of ECM is mainly regulated by matrix metalloproteinases. The expression of matrix metalloproteinase-1 is negatively correlated with cooking loss and positively correlated with hydraulic performance [50]. Collagen is an abundant connective tissue protein that is an important factor in the tenderness and texture of the meat and is well resistant to physical damage during cooking [51].…”