Expression of MMP-2 and TIMP-1 in Renal Tissue of Patients with Chronic Active Antibody-mediated Renal Graft Rejection

Yan, Qiang; Sui, Weiguo; Wang, Baoyao; Zou, Hequn; Zou, Guimian; Luo, Hao

doi:10.1186/1746-1596-7-141

Cited by 25 publications

(21 citation statements)

References 7 publications

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“…In our previous study [27], we found that matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1) were important cytokines for ECM synthesis and degradation, and the excess accumulation of ECM is the main pathological mechanism of fibrosis in antibody-mediated renal graft rejection. Our present study reveals that TGF-beta1 induced expression of mmp-9 may induce mononuclear cells infiltration in the interstitium of allografts and SMC replication.…”

Section: Discussionmentioning

confidence: 99%

Dramatic early event in chronic allograft nephropathy: increased but not decreased expression of MMP-9 gene

Shi

Ding

et al. 2013

Diagn Pathol

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ObjectiveThe infiltration of mononuclear cells and replication and migration of smooth muscle cells (SMCs) from media into the intima in the vascular wall are the cardinal pathological changes in the early stage of chronic allograft nephropathy (CAN). But the mechanism is unclear. Therefore we investigated the role of matrix metalloproteinase 9 (MMP-9) and its interaction with TGF-beta1, tubulointerstitial mononuclear cells infiltration and migration of SMCs in the early stage of CAN.MethodsKidneys of Fisher (F334) rats were orthotopically transplanted into bilaterally nephrectomized Lewis (LEW) recipients. To suppress an initial episode of acute rejection, rats were briefly treated with cyclosporine A (1.5 mg/kg/day) for the first 10 days. Animals were harvested at 12 weeks after transplantation for histological, immunohistochemistry and molecular biological analysis.ResultsThe expression of MMP-9 was up-regulated in interstitium and vascular wall in the early stage of CAN, where there were interstitial mononuclear cells infiltration and SMCs migration and proliferation. Moreover the expression of MMP-9 were positively correlated with the degree of interstitial mononuclear cells infiltration, the quantity of SMCs in arteriolar wall, and also the increased TFG-beta1 expression in the tubulointerstitium and arteriolar wall.ConclusionsMMP-9 may play an important role in the mechanism of pathological changes during the earlier period of CAN.Virtual SlidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1582313332832700.

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Section: Discussionmentioning

confidence: 99%

Dramatic early event in chronic allograft nephropathy: increased but not decreased expression of MMP-9 gene

Shi

Ding

et al. 2013

Diagn Pathol

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“…After PBS washes, slices were incubated with a HRP-conjugated secondary antibody at 37°C for 30 min before staining with DAB and hematoxylin. Semi-quantitative analysis of target protein expression was performed as previously described3 [24]. Briefly, ten randomly selected fields (400x) per kidney were captured and each field was imaged with one glomerulus in the center of the picture.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic Effect of Alprostadil in Diabetic Nephropathy: Possible Roles of Angiopoietin-2 and IL-18

Chen

Zhang

et al. 2014

Cell Physiol Biochem

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Background/Aims: To investigate the role of angiopoietin-2 (Ang-2) and IL-18 in the pathogenesis of diabetic nephropathy (DN) and the molecular mechanisms through which alprostadil protects renal function. Methods: DN was induced by streptozotocin and intraperitoneal injection of alprostadil was given to diabetic mice. After 2, 4 and 8 weeks of alprostadil treatment, the mRNA and protein expression of kidney Ang-2 and IL-18 were detected by reverse transcription PCR, Western blot and immunohistochemistry analyses. Mouse glomerular endothelial cells (GEnCs) were cultured in high glucose and treated with alprostadil. After transfection with an Ang-2-pcDNA and Ang-2-siRNA, both Ang-2 and IL-18 expression were measured by Western blot analyses. Results: Alprostadil treatment caused a significant decrease in the renal damage parameters. Both Ang-2 and IL-18 were significantly increased in DN mice and in GEnCs cultured in high glucose; however, their expression was greatly reduced by alprostadil treatment. Ang-2 could also increase IL-18 expression in cultured endothelial cells under high glucose, and this response was partially blocked by Ang-2 siRNA. Conclusions: Ang-2 and IL-18 may be associated with the development and progression of DN in mice. Alprostadil treatment can protect renal function by reducing proteinuria. These effects are mediated, at least in part, through down-regulation of Ang-2 and IL-18 expression.

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“…TIA-1 is the granule component responsible for inducing DNA fragmentation and apoptosis in cytolytic lymphocyte targets. In acute rejection after kidney transplantation, the quantity and intensity of TIA-1 expression increase, and the degree of this variation can reflect rejection severity to some extent [15,28,29]. However, the diagnostic value of TIA-1 in acute rejection after liver transplantation has not been determined.…”

Section: Discussionmentioning

confidence: 99%

Local distribution analysis of cytotoxic molecules in liver allograft is helpful for the diagnosis of acute cellular rejection after orthotopic liver transplantation

et al. 2012

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BackgroundAs it is often difficult for a transplant pathologist to make a definite diagnosis of acute cellular rejection (ACR) by routine morphological analysis of liver allograft biopsy, supplementary methods and objective markers are needed to facilitate this determination.MethodsTo evaluate the diagnostic value of cytotoxic molecules in ACR episodes, immunohistochemical staining for perforin, granzyme B and T-cell intracellular antigen-1 (TIA-1) were performed in liver allograft biopsies. The positive cells in the portal tract area and lobules were counted separately to investigate the distribution of the cytotoxic molecules.ResultsThe immunohistochemical study showed that the overall positive rates for the three markers were not significantly different between the ACR and non-ACR groups. However, in the portal tract area, perforin-, granzyme B- and TIA-1-positive cells in the ACR group were significantly more than those in the non-ACR groups. In the lobules, perforin- and granzyme B-positive cells in the ACR group were significantly more than those in the biliary complication and opportunistic infection groups, while TIA-1-positive cells was significantly fewer than those in non-ACR groups. The numbers of positive cells in the portal tract area correlated with the rejection activity index of ACR.ConclusionsThese results indicate that, though the overall positive rates have nonsense in ACR diagnosis, the quantification and local distribution analysis of cytotoxic molecule positive cells in liver tissue is helpful for differential diagnosis and severity evaluation of ACR following liver transplantation.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2292255038100487

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Expression of MMP-2 and TIMP-1 in Renal Tissue of Patients with Chronic Active Antibody-mediated Renal Graft Rejection

Cited by 25 publications

References 7 publications

Dramatic early event in chronic allograft nephropathy: increased but not decreased expression of MMP-9 gene

Dramatic early event in chronic allograft nephropathy: increased but not decreased expression of MMP-9 gene

Therapeutic Effect of Alprostadil in Diabetic Nephropathy: Possible Roles of Angiopoietin-2 and IL-18

Local distribution analysis of cytotoxic molecules in liver allograft is helpful for the diagnosis of acute cellular rejection after orthotopic liver transplantation

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