Expression of Molecular Markers in Tumours of Patients with Breast Cancer

Abstract

The expression of transcription factor NF-κB (p50 and p65), ER, PR, Her2/neu, Ki-67, p53, Bcl-2 and E-cadherin in tumor tissue of 132 patients with breast cancer was investigated by immunohistochemistry. As a result of researches the correlation between Grade and expression of ER, PR, Bcl-2, Ki-67 and p65-subunit of NF-κB was defined. The level of proliferation was correlated with ER, PR, Bcl-2, p53 and Her2/neu expression. Also the correlation between some of investigated markers was found. Thus, high level of NF-κB nuclear expression was associated with Her2/neu hyperexpression and negative status of ER, PR and Bcl-2. These data may indicate poor prognosis and resistance to chemotherapy in breast cancer patients with activated NF-κB, but it requires further research and retrospective analysis of large number of patients.

Ki-67, p53 and BCL-2 Expressions and their Association with Clinical Histopathology of Breast Cancer among Women in Tanzania

“…Shapochka et al . 26 also reported the association between Ki-67 with tumor grade but their conclusion regarding the correlation between p53 and tumor grade was not observed in this current study. Further, Filip Čečka et al .…”

“…BCL-2 protein, on the other hand, is encoded by the BCL-2 gene and plays an anti-apoptotic role by inhibiting cell death, which results in prolonged cell survival. BCL-2 is overexpressed in many cancers, including breast cancer as observed in our study, and contributes to tumor initiation, progression, and resistance to therapy 17,26,32–34 . However, the antagonizing role (negative correlation) between p53 and BCL-2 observed in the current study could be understood through a mechanistic study involving controlling the inhibition of cell proliferation and apoptosis 35 .…”

“…Shapochka et al . 26 affirmed in their investigation that the level of tumor proliferation was inversely correlated with expression of hormonal receptors and BCL-2. Nevertheless, this theory needs further research to support it.…”

“…The relationships between Ki-67 expression, histopathological grading and BCL-2 expression in BC have been described by numerous studies as molecular markers mostly associated with malignant tissues 20–25 , intermediate grade, negative/low p53 (p53-) status, high levels of BCL-2 (BCL-2) expression, suggesting to be sensitive to hormonal therapy 26,27 . However, some findings were not obvious in our study, because of non-significant statistical results that are most likely due to the low power of our study.…”

Association of Ki-67, p53 and BCL-2 bio-markers expression with Clinic-Histopathology of breast cancer among women in Tanzania

“…p53 is a tumor suppressor gene which normally limits cell development by monitoring quick cells division into new cells and rectifying DNA mismatched, but it is also a significant prognostic marker in early breast tumor screening. p53 expression has been demonstrated by diverse authors to be part of up to 50% of breast tumor through DNA impairment, oncogene activation, hypoxia, oxidase stress, viral infection (Shapochka, Zaletok & Gnidyuk, 2013). BCL-2 is responsible for promoting cell survival, inhibiting the action of pro-apoptotic antigen, and is expressively associated with hormone receptor status, because of its expression in ordinary breast glandular epithelium and estrogen-upregulated.…”