Expression of Molecules Involved in Antigen Presentation and T Cell Activation (HLA‐DR, CD80, CD86, CD44 and CD54) by Cultured Human Osteoblasts

Reyes-Botella, Candela; Montes, María José Sánchez; Vallecillo-Capilla, Manuel; Olivares, Enrique G.; Ruíz, Concepción

doi:10.1902/jop.2000.71.4.614

Cited by 50 publications

(60 citation statements)

References 16 publications

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“…Thus, it was not necessary to have intact, viable Salmonella cells to stimulate osteoblasts to express CXCL10. Furthermore, this result was consistent with the results of a limited number of previous publications (23,25,29,31,48,49,53) demonstrating that bacterial LPS can stimulate osteoblast secretion of select chemokines, cytokines, or nitric oxide. Interestingly, the TH1-and TH2-derived cytokines IFN-␥ and IL-4 could also induce CXCL10 secretion (Fig.…”

Section: Vol 70 2002 Tlr4-mediated Cxcl10 Secretion By Infected Ostsupporting

confidence: 91%

Bacterium-Induced CXCL10 Secretion by Osteoblasts Can Be Mediated in Part through Toll-Like Receptor 4

et al. 2002

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Two common pathogens known to cause bone infection, Salmonella and Staphylococcus aureus, were investigated to determine their abilities to induce chemokine expression in cultured mouse and human osteoblasts. While these cells are responsible for bone formation, we were surprised to find that they could respond to bacterial infection by upregulating expression of the chemokine CXCL10 (IP-10). However, there were significant differences in the abilities of the gram-negative bacterium Salmonella and the gram-positive bacterium S. aureus to induce expression of CXCL10. Reverse transcription-PCR and enzyme-linked immunosorbent assay analyses showed high levels of Salmonella-induced CXCL10 mRNA and protein expression, respectively, whereas the osteoblast response to S. aureus was significantly less. Consistent with these findings, Salmonelladerived lipopolysaccharide (LPS), but not S. aureus-derived peptidoglycan, could induce expression of CXCL10. An antibody against toll-like receptor 4 (TLR4) could block the LPS-induced CXCL10 production, demonstrating the functional expression of TLR4 by osteoblasts. Despite the inducible nature of TLR2 mRNA expression by bacterium-infected osteoblasts, peptidoglycan failed to stimulate CXCL10 secretion. Immunofluorescent staining of bacterium-infected calvaria (i.e., skull bone) demonstrated the presence of CXCL10 in osteoblasts. The fact that osteoblasts did not express CXCR3 mRNA, whereas T lymphocytes can express high levels of this receptor, suggests that osteoblast-derived CXCL10 may recruit T lymphocytes to the sites of bone infections.

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Section: Vol 70 2002 Tlr4-mediated Cxcl10 Secretion By Infected Ostsupporting

confidence: 91%

Bacterium-Induced CXCL10 Secretion by Osteoblasts Can Be Mediated in Part through Toll-Like Receptor 4

et al. 2002

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“…Although the immunosuppressive function of DOC is promising, it cannot be ignored that DOC gradually expressed MHC class II and lost their suppressive activity in vivo. It is reported that POC expressed all immune surface markers which can elicit an immune response, and they are able to act as APCs (42)(43)(44)(45)(46). Hence, it is predicted that DOC in vivo are going the way their progeny normally does.…”

Section: Discussionmentioning

confidence: 99%

“…1), and their capacity to differentiate into osteogenic and chondrogenic cells in vitro (data and photos not shown). Previous studies reported that MSC are MHC class II-negative and are able to suppress lymphocyte proliferation in MLC, while POC are MHC class II-positive and responsible for T cell activation (42). Hence, we therefore examined MHC class II expression on DOC first.…”

Section: Immunogenicity and Immunosuppression Assessment Of Doc From mentioning

confidence: 99%

The Immunogenicity and Immunomodulatory Function of Osteogenic Cells Differentiated from Mesenchymal Stem Cells

Liu

Kemeny

Heng

et al. 2006

The Journal of Immunology

191

165

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Multipotent mesenchymal stem cells (MSC) are reported to be immunoprivileged as well as immunosuppressive. Hence, they are ideal candidates for allogeneic transplantation to induce regeneration of diseased tissues and organs. However, it is not known whether MSC would retain their immunoprivileged and immunomodulatory properties after differentiating into the local cell types of the transplantation site. This study sought to investigate this question with a novel New Zealand White rabbit osteogenesis model. Results showed that osteogenic cells differentiated from MSC (DOC) in vitro did not express the MHC class II molecule, were incapable of inducing allogeneic lymphocyte proliferation in mixed lymphocyte culture or generating CTL, were inhibitory in ongoing lymphocyte proliferation, and secreted anti-inflammatory cytokines (IL-10 and TGF-β). There was a significantly higher secretion of IL-10 by DOC than that by MSC, while there was no significant difference between the TGF-β secretion of MSC and DOC in vitro. However, after IFN-γ treatment, TGF-β secretion by DOC significantly decreased despite the increased production by MSC. Four weeks after local DOC implantation, despite MHC class II expression, second-set allogeneic skin rejection showed similar survival to first-set allogeneic skin rejection and DOC appeared to function as osteoblasts. In conclusion, DOC retained their immunoprivileged and immunomodulatory properties in vitro, but the latter was lost following transplantation.

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“…28 A strong and constitutive expression of CD10 and CD44 was described on cultured human osteoblasts in flow cytometry studies [29][30][31] and on human bone tissue sections using immunohistochemistry. 32 Other authors described expression of CD44 antigen, a multifunctional adhesion molecule that binds to extracellular matrix molecules such as hyaluronate, 33 type I collagen, and fibronectin 34 during the change from osteoblast to osteocyte.…”

Section: Discussionmentioning

confidence: 99%

“…35 However, the presence of CD44 in osteoblastic cells at various maturational stages was assessed in rat fetal bone at the mRNA and protein levels. 36 The presence of CD54 on osteoblasts is well documented, 30,37 and its upregulation on osteoblastic cells consecutive to CD44 stimulation by fragmented hyaluronan was shown to facilitate osteoclastogenesis. 38,39 In accordance with our findings, TNF-a and IFN-g signal transduction pathways activate CD54.…”

Section: Discussionmentioning

confidence: 99%

In VitroCharacterization of Immune-Related Properties of Human Fetal Bone Cells for Potential Tissue Engineering Applications

Montjovent

Bocelli‐Tyndall

Scaletta

et al. 2009

Tissue Engineering Part A

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We describe herein some immunological properties of human fetal bone cells recently tested for bone tissueengineering applications. Adult mesenchymal stem cells (MSCs) and osteoblasts were included in the study for comparison. Surface markers involved in bone metabolism and immune recognition were analyzed using flow cytometry before and after differentiation or treatment with cytokines. Immunomodulatory properties were studied on activated peripheral blood mononuclear cells (PBMCs). The immuno-profile of fetal bone cells was further investigated at the gene expression level. Fetal bone cells and adult MSCs were positive for Stro-1, alkaline phosphatase, CD10, CD44, CD54, and b2-microglobulin, but human leukocyte antigen (HLA)-I and CD80 were less present than on adult osteoblasts. All cells were negative for HLA-II. Treatment with recombinant human interferon gamma increased the presence of HLA-I in adult cells much more than in fetal cells. In the presence of activated PBMCs, fetal cells had antiproliferative effects, although with patterns not always comparable with those of adult MSCs and osteoblasts. Because of the immunological profile, and with their more-differentiated phenotype than of stem cells, fetal bone cells present an interesting potential for allogeneic cell source in tissue-engineering applications.

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Expression of Molecules Involved in Antigen Presentation and T Cell Activation (HLA‐DR, CD80, CD86, CD44 and CD54) by Cultured Human Osteoblasts

Abstract: Our results suggest that osteoblastic cells or a subpopulation of these cells may have immune functions in bone. Further studies in which immune functions are assessed will be needed to test this hypothesis.

Cited by 50 publications

References 16 publications

Bacterium-Induced CXCL10 Secretion by Osteoblasts Can Be Mediated in Part through Toll-Like Receptor 4

Bacterium-Induced CXCL10 Secretion by Osteoblasts Can Be Mediated in Part through Toll-Like Receptor 4

The Immunogenicity and Immunomodulatory Function of Osteogenic Cells Differentiated from Mesenchymal Stem Cells

In VitroCharacterization of Immune-Related Properties of Human Fetal Bone Cells for Potential Tissue Engineering Applications

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