Expression of Monocyte/Macrophage Markers (CD13, CD14, CD68) on Human Keratinocytes in Healthy and Diseased Skin

Hunyadi, J.; Simon, Miklós; Kenderessy, Anna Sz.

doi:10.1111/j.1346-8138.1993.tb01295.x

Cited by 32 publications

(16 citation statements)

References 19 publications

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“…, 1997). CD14 expression is also increased in keratinocytes in psoriatic skin, as has been shown using immunohistochemistry, while the keratinocytes of non‐involved psoriatic skin and normal skin are CD14‐negative (Hunyadi et al. , 1993).…”

Section: Introductionmentioning

confidence: 65%

Promoter region polymorphism of the CD14 gene (C–159T) is not associated with psoriasis vulgaris

Karhukorpi

Ikäheimo²,

Karvonen

et al. 2002

European Journal of Immunogenetics

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Keratinocytes of psoriatic skin show aberrant expression of membrane-bound CD14 (mCD14). In addition, soluble CD14 (sCD14) is elevated in the sera of psoriatic patients. The mechanisms leading to increased CD14 expression and secretion in psoriasis are poorly understood. A bi-allelic polymorphism in the promoter region of the CD14 gene controls CD14 expression on monocytes and sCD14 levels in the sera of healthy subjects. In this context, we explored the CD14 promoter region genotypes of 63 Finnish patients with psoriasis and 126 non-psoriatic controls using a new ARMS-PCR method. No differences in the CD14 genotype frequencies were found between the groups. Thus, our results suggest that the enhanced CD14 expression in psoriasis is not attributable to functional variants of CD14 (-159C/T).

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Section: Introductionmentioning

confidence: 65%

Promoter region polymorphism of the CD14 gene (C–159T) is not associated with psoriasis vulgaris

Karhukorpi

Ikäheimo²,

Karvonen

et al. 2002

European Journal of Immunogenetics

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“…In fact, our preliminary analysis showed that APN gene knockdown in dermal fibroblasts abolished many of KCM‐induced gene expression changes in these cells. At the same time, the reciprocal induction of keratinocyte APN expression by fibroblasts illustrates the importance of epidermal–mesenchymal interaction in skin diseases hallmarked by abnormal APN expression in keratinocytes (Hunyadi et al, 1993), and provides supporting evidence for the proposed role of APN in epidermization (keratinocyte growth and differentiation) which is profoundly influenced by the presence of fibroblasts (Robert et al, 1994; Thielitz et al, 2004).…”

Section: Discussionmentioning

confidence: 87%

Paracrine regulation of fibroblast aminopeptidase N/CD13 expression by keratinocyte-releasable stratifin

Lai¹,

Ghaffari²,

Li³

et al. 2011

J. Cell. Physiol.

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As wound healing proceeds into the tissue remodeling phase, cellular interactions become dominated by the interplay of keratinocytes with fibroblasts in the skin, which is largely mediated through paracrine signaling and greatly affects the molecular constitution of the extracellular matrix. We have recently identified aminopeptidase N (APN)/CD13 as a potential fibroblast receptor for 14-3-3 sigma (also known as stratifin), a keratinocyte-releasable protein with potent matrix metalloproteinase 1 (MMP1) stimulatory activity. The present study demonstrates that the expression of APN on dermal fibroblasts is regulated through paracrine signaling by keratinocyte-derived soluble factors. By using an in vitro keratinocyte-fibroblast co-culture system, we showed that APN expression in dermal fibroblasts is induced in the presence of keratinocytes or in response to keratinocyte-conditioned medium. Conditioned medium collected from differentiated keratinocytes further increases APN protein production, suggesting an amplified stimulatory effect by keratinocyte differentiation. Recombinant stratifin potently induces APN synthesis in a dose-dependent manner. A consistent correlation between the protein expression levels of APN and MMP1 was also observed. These results confirm paracrine regulation of APN expression in dermal fibroblasts by keratinocyte-derived stimuli, in particular stratifin, and provide evidence that APN may serve as a target in the regulation of MMP1 expression in epidermal-mesenchymal communication.

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“…Thus an intricate, cytokine‐based signalling network in the epidermis can form the basis of a cascading inflammatory response in diverse skin diseases. Keratinocytes also possess the ability to synthesize and express cell surface moieties characteristic of effector or accessory cells of the immune system, 67 and there is increasing evidence of the ability of sugars to influence cell‐to‐cell communication among keratinocytes and immune system activity.…”

Section: Immunomodulatory Properties Of Monosaccharidesmentioning

confidence: 99%

Role of sugars in surface microbe–host interactions and immune reaction modulation

Lloyd

Viac

Werling

et al. 2007

Veterinary Dermatology

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Sugars in the form of monosaccharides, oligosaccharides, polysaccharides and glycoconjugates (glycoproteins, glycolipids) are vital components of infecting microbes and host cells, and are involved in cell signalling associated with modulation of inflammation in all integumental structures. Indeed, sugars are the molecules most commonly involved in cell recognition and communication. In skin, they are essential to epidermal development and homeostasis. They play important roles in microbial adherence, colonization and biofilm formation, and in virulence. Two groups of pathogen recognition receptors, C-type lectins (CTL) and their receptors (CTLR), and the Toll-like receptors enable the host to recognize pathogen-associated molecular patterns (PAMPs), which are mainly glycolipids. The CTLs can recognize a wide variety of bacteria, fungi and parasites and are important in phagocytosis and endocytosis. TLRs are expressed on the surfaces of a variety of cells, including keratinocytes, dendritic cells, monocytes and macrophages; they play a major role in innate immunity. Interaction of TLRs with PAMPs initiates a cascade of events leading to production of reactive oxygen intermediates, cytokines and chemokines, and promotes inflammation. Exogenous sugars can block carbohydrate receptors and competitively displace bacteria from attachment to cells, including keratinocytes. Thus sugars may provide valuable adjunctive anti-inflammatory and/or antimicrobial treatment. A promising approach is the use of a panel of carbohydrate derivatives with anti-adhesive efficacy against bacteria frequently involved in diseases affecting skin and other epithelia. More complete characterization of sugar receptors and their ligands will provide further keys to use of carbohydrates in immunomodulation and infection control in skin.

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Expression of Monocyte/Macrophage Markers (CD13, CD14, CD68) on Human Keratinocytes in Healthy and Diseased Skin

Cited by 32 publications

References 19 publications

Promoter region polymorphism of the CD14 gene (C–159T) is not associated with psoriasis vulgaris

Promoter region polymorphism of the CD14 gene (C–159T) is not associated with psoriasis vulgaris

Paracrine regulation of fibroblast aminopeptidase N/CD13 expression by keratinocyte-releasable stratifin

Role of sugars in surface microbe–host interactions and immune reaction modulation

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