Expression of mRNA encoding cAMP‐specific phosphodiesterase isoforms in rat parotid glands

Imai, Akane; Nashida, Tomoko; Shimomura, Hiromi

doi:10.1080/15216549600201813

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Salivary gland-specific CysS plays a major role in protecting the mouth against bacteria and viruses 5,6 . Among the PDE gene families, PDE3A and PDE4D are the dominant subfamilies present in the rat salivary glands [7][8][9][10][11] . Aquaporin is a transmembrane water channel that contributes to membrane water permeability.…”

Section: Introductionmentioning

confidence: 99%

Changes in Salivary Gene Expression in F344 Rats Exposed to Theophylline

et al. 2006

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Exposure to theophylline, a phosphodiesterase (PDE) inhibitor, increases the basophilic basal part and conspicuous nucleoli in salivary acini. These histological findings imply increased gene expression. The purpose of this study was to examine the effect of theophylline on the expression of representative genes in the salivary glands which are regulated by cyclic AMP. Male F344 rats received saline or theophylline (50 mg/kg) intraperitoneally. Parotid and submaxillary glands were isolated from rats at 4, 8, and 24 h post-administration, and gene expression for the principal secretory proteins, amylase 1 (AMY1) and cystatin S (CysS), dominant PDE subfamilies 3A and 4D, and a water channel, aquaporin 5 (AQP5) were quantified by RT-PCR. The cyclic AMP concentration was determined by enzyme-immunoassay. Theophylline exposure resulted in a transient increase in mRNA expression for AMY1 in the parotid gland, CysS in the submaxillary gland, and PDE3A in both salivary glands. The cyclic AMP concentration was elevated in both glands. PDE4D and AQP5 gene expressions were not altered in either gland. Our results suggest that theophylline induces cyclic AMP signals through PDE inhibition, leading to enhanced gene transcription and subsequent histological changes. (J Toxicol Pathol 2006; 19: 47-52)

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Section: Introductionmentioning

confidence: 99%

Changes in Salivary Gene Expression in F344 Rats Exposed to Theophylline

et al. 2006

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“…Salivary gland-specific CysS plays a major role in protecting the mouth against bacteria and viruses [1,10,11]. Among the PDE gene families, PDE3A and PDE4D are the dominant sub-families expressed in the rat salivary glands [2][3][4][7][8][9].…”

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confidence: 99%

Changes in Submaxillary Gland Gene Expression in F344 Rats by Multiple Dosing of Theophylline

et al. 2006

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Theophylline (1,3-dimethylxyanthine) is a non-specific phosphodiesterase (PDE) inhibitor commonly used for the treatment of bronchial asthma. Since PDE enzymes hydrolyze cyclic AMP, theophylline enhances the cyclic AMP signal by inhibiting this hydrolysis. Cyclic AMP works as an intracellular second messenger and provides the signal for smooth muscle relaxation in the bronchial airways. Therapeutic doses of theophylline range from 400 to 600 mg/day.Repeated administration of toxic doses of theophylline evokes submaxillary gland hypertrophy characterized by an increased number of secretory vesicles in the acinar epithelial cells [5,13]. In the field of toxicology, it has been reported that repeated administration of PDE inhibitors produces salivary gland

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