Expression of mRNA for keratinocyte growth factor and its receptor in human endometrium

Siegfried, Sari; Pekonen, Fredrika; Nyman, Tuulikki; Ämmälä, Martti

doi:10.3109/00016349509024400

Cited by 22 publications

(17 citation statements)

References 20 publications

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“…57–59 In the uterus, KGF expression is lowest during the proliferative phase (predominantly estradiol) and highest during the secretory phase (predominately progesterone). 58, 59 Previous work from our laboratory has shown that, in vitro , estradiol inhibits mouse uterine epithelial cell constitutive, as well as LPS-induced, CCL20 secretion. 44 More recently, our laboratory has demonstrated that CCL20 and CXCL1 fluctuate during the estrous cycle of the mouse.…”

Section: Introductionmentioning

confidence: 99%

Estradiol Regulation of Constitutive and Keratinocyte Growth Factor-Induced CCL20 and CXCL1 Secretion by Mouse Uterine Epithelial Cells

Haddad

Wira

2014

Am J Reprod Immunol

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Problem Estradiol can directly affect epithelial cells or indirectly affect epithelial cells via stromal fibroblast secretion of growth factors, such as keratinocyte growth factor (KGF). The purpose of the present study was to determine if estradiol regulates constitutive as well as KGF-induced uterine epithelial cell secretion of CCL20 and CXCL1. Method of Study Freshly isolated and polarized uterine epithelial cells from Balb/c mice were cultured with estradiol in the presence or absence of KGF. CCL20 and CXCL1 were measured by ELISA. Results Estradiol inhibited CCL20 secretion by freshly isolated and polarized uterine epithelial cells in the presence or absence of KGF. Unexpectedly, it enhanced KGF-induced CXCL1 secretion beyond that seen with KGF alone. Estradiol increased CXCL1 secretion at 24 hours and inhibited CCL20 at 48 hours. The effects of estradiol are specific in that progesterone, cortisol, dihydrotestosterone, and aldosterone had no effect on either CCL20 or CXCL1 secretion. The inhibitory effect of estradiol on CCL20 secretion was reversed with ICI 182,780, an estrogen-receptor antagonist, indicating that this effect is estrogen receptor-mediated. Conclusions Our data indicate that estradiol is important in regulating the effects of KGF on mouse uterine epithelial cell secretion of CCL20 and CXCL1.

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Section: Introductionmentioning

confidence: 99%

Estradiol Regulation of Constitutive and Keratinocyte Growth Factor-Induced CCL20 and CXCL1 Secretion by Mouse Uterine Epithelial Cells

Haddad

Wira

2014

Am J Reprod Immunol

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“…Due to ligand and receptor expression patterns, it is thought that KGF functions as a paracrine mediator of stromal fibroblast-epithelial cell interactions [73,78,79]. Stage of the menstrual cycle influences the expression of KGF and KGFR in the human and primate endometria suggesting that KGF is under hormonal control [79][80][81]. KGF expression appears to be lowest during the proliferative phase and peaks during the late-secretory phase [80,81].…”

Section: Keratinocyte Growth Factormentioning

confidence: 99%

“…Stage of the menstrual cycle influences the expression of KGF and KGFR in the human and primate endometria suggesting that KGF is under hormonal control [79][80][81]. KGF expression appears to be lowest during the proliferative phase and peaks during the late-secretory phase [80,81]. In contrast to KGF expression in the endometrium, mammary gland treatment with E 2 increases KGF mRNA expression in a dose-dependent manner while progesterone is needed to stabilize KGFR mRNA [78,82].…”

Section: Keratinocyte Growth Factormentioning

confidence: 99%

Innate Immunity in the Female Reproductive Tract: Role of Sex Hormones in Regulating Uterine Epithelial Cell Protection Against Pathogens

Ochiel

Fahey²,

Ghosh³

et al. 2008

CWHR

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The mucosal immune system in the upper female reproductive tract is uniquely prepared to maintain a balance between the presence of commensal bacteria, sexually transmitted bacterial and viral pathogens, allogeneic spermatozoa, and an immunologically distinct fetus. At the center of this dynamic system are the epithelial cells that line the Fallopian tubes, uterus, cervix and vagina. Epithelial cells provide a first line of defense that confers continuous protection, by providing a physical barrier as well as secretions containing bactericidal and virucidal agents. In addition to maintaining a state of ongoing protection, these cells have evolved to respond to pathogens, in part through Toll-like receptors (TLRs), to enhance innate immune protection and, when necessary, to contribute to the initiation of an adaptive immune response. Against this backdrop, epithelial cell innate and adaptive immune function is modulated to meet the constraints of procreation. The overall goal of this review is to focus on the dynamic role of epithelial cells in the upper reproductive tract, with special emphasis on the uterus, to define the unique properties of these cells as they maintain homeostasis in preparation for successful fertilization and pregnancy while at the same time confer protection against sexually transmitted infections, which threaten to compromise women's reproductive health and survival. By understanding the nature of this protection and the ways in which innate and adaptive immunity are regulated by sex hormones, these studies provide the opportunity to contribute to the foundation of information essential for ensuring reproductive health.

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“…The same applies to the expression of heparin-binding epidermal growth factor (Zhang et al 1994). Studies indicate that keratinocyte growth factor is a progestomedin expressed by stromal cells, whereas expression of its receptor is estrogen-dependent (Koji et al 1994, Siegfried et al 1995. Recent results suggest that progesterone is also a potent stimulator of basic fibroblast growth factor in the rat uterus (Rider et al 1997).…”

Section: Introductionmentioning

confidence: 97%

Upregulation of the angiogenic factor heparin affin regulatory peptide by progesterone in rat uterus

Milhiet

Vacherot²,

Caruelle³

et al. 1998

Journal of Endocrinology

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Heparin affin regulatory peptide (HARP), also named pleiotropin, is a secreted polypeptide that belongs to a new family of heparin-binding growth/differentiation factors. In this study, we investigated the expression and distribution of HARP mRNA and protein in rat uterus. Semi-quantitative reverse transcriptase PCR experiments showed variations in HARP mRNA levels throughout the estrous cycle, with a maximum during diestrus, pointing to hormonal regulation of HARP mRNA expression. Uterine expression of HARP mRNA was studied in ovariectomized animals treated with 17 -estradiol, progesterone alone or progesterone and RU486. In these experiments, progesterone upregulated HARP mRNA expression. Induction was observed 6 h after progesterone injection and was inhibited by RU486 treatment. In contrast, after 17 -estradiol injection, a slight decrease in HARP mRNA expression was observed. In situ hybridization studies with digoxigenin-labeled DNA probe revealed that HARP mRNA was present in smooth muscle cells of both myometrium and blood vessels and also in endothelial cells from endometrium. Immunohistochemical studies showed that HARP expression was not limited to cells that expressed HARP mRNA, but also occurred in both the luminal and glandular epithelium even though its transcript was never detected. We conclude that HARP may mediate the effects of progesterone on the homeostasis and vascularization of uterine tissue.

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Expression of mRNA for keratinocyte growth factor and its receptor in human endometrium

Abstract: The results suggest that keratinocyte growth factor mRNA expression is progesterone dependent, whereas keratinocyte growth factor receptor mRNA expression seems to be more estrogen than progesterone dependent.

Cited by 22 publications

References 20 publications

Estradiol Regulation of Constitutive and Keratinocyte Growth Factor-Induced CCL20 and CXCL1 Secretion by Mouse Uterine Epithelial Cells

Estradiol Regulation of Constitutive and Keratinocyte Growth Factor-Induced CCL20 and CXCL1 Secretion by Mouse Uterine Epithelial Cells

Innate Immunity in the Female Reproductive Tract: Role of Sex Hormones in Regulating Uterine Epithelial Cell Protection Against Pathogens

Upregulation of the angiogenic factor heparin affin regulatory peptide by progesterone in rat uterus

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