Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts

Jiroutová, Alena; Slavkovský, Rastislav; Cermáková, M; Majdiaková, Lenka; Hanovcová, I; Bolehovská, Radka; Hajzlerová, Milena; Radilová, Hana; Ruszova, Ema; Kanta, J

doi:10.1016/j.etp.2006.06.005

Cited by 7 publications

(7 citation statements)

References 42 publications

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“…A similar approach was employed in a study analyzing gene expression patterns in human stellate cells isolated from human fibrotic liver, compared with normal primary stellate cells (561). In another report comparing stellate cells from normal rat liver, cirrhotic liver, and normal cells activated by growth on plastic, there were distinct differences between normal cells and those activated by plastic, but fewer differences between stellate cells from normal versus cirrhotic liver (275).…”

Section: H Transcriptome and Proteome Analysesmentioning

confidence: 99%

Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver

Friedman

2008

Physiological Reviews

2,424

2,549

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The hepatic stellate cell has surprised and engaged physiologists, pathologists, and hepatologists for over 130 years, yet clear evidence of its role in hepatic injury and fibrosis only emerged following the refinement of methods for its isolation and characterization. The paradigm in liver injury of activation of quiescent vitamin A-rich stellate cells into proliferative, contractile, and fibrogenic myofibroblasts has launched an era of astonishing progress in understanding the mechanistic basis of hepatic fibrosis progression and regression. But this simple paradigm has now yielded to a remarkably broad appreciation of the cell's functions not only in liver injury, but also in hepatic development, regeneration, xenobiotic responses, intermediary metabolism, and immunoregulation. Among the most exciting prospects is that stellate cells are essential for hepatic progenitor cell amplification and differentiation. Equally intriguing is the remarkable plasticity of stellate cells, not only in their variable intermediate filament phenotype, but also in their functions. Stellate cells can be viewed as the nexus in a complex sinusoidal milieu that requires tightly regulated autocrine and paracrine cross-talk, rapid responses to evolving extracellular matrix content, and exquisite responsiveness to the metabolic needs imposed by liver growth and repair. Moreover, roles vital to systemic homeostasis include their storage and mobilization of retinoids, their emerging capacity for antigen presentation and induction of tolerance, as well as their emerging relationship to bone marrow-derived cells. As interest in this cell type intensifies, more surprises and mysteries are sure to unfold that will ultimately benefit our understanding of liver physiology and the diagnosis and treatment of liver disease.

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Section: H Transcriptome and Proteome Analysesmentioning

confidence: 99%

Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver

Friedman

2008

Physiological Reviews

2,424

2,549

View full text Add to dashboard Cite

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“…Quiescent HSC express type III and type IV collagen, activated HSC produce increasing amounts of type I collagen (15). HSC activation observed in vivo can be reproduced to a large extent by cultivation of these cells on plastic surface (5,12). Excessive deposition of ECM, especially of collagen I, may influence morphology, function and fate of HSC as shown by cultivation of these cells on collagen gel (22).…”

Section: Introductionmentioning

confidence: 99%

“…The resulting cell suspension was centrifuged on Optiprep (Axis-Shield, Oslo, Norway) density gradient as described by Jiroutová et al (12). Cell viability determined by trypan blue staining was greater than 95%.…”

Section: Animal Treatment Cell Isolation Culture and Proliferation mentioning

confidence: 99%

“…The cells cultured for 2 days were regarded as quiescent (12). They were cultivated on plastic or on collagen gel for 7 days and thereafter were treated with 1.5 μM GLT (Sigma) for 5 hours (18), 75 μM CHX (Calbiochem Merck, Prague) for 14 hours (16) or 5 µg/ml (0.01 μM) CYT D (Sigma) for 24 hours (17).…”

Section: Animal Treatment Cell Isolation Culture and Proliferation mentioning

confidence: 99%

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Effect of Collagen I Gel on Apoptosis of Rat Hepatic Stellate Cells

Bittnerová

Jiroutová

Rudolf

et al. 2013

Acta Med. (Hradec Kralove, Czech Repub.)

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Summary: Activated hepatic stellate cells (HSC) are a major source of fibrous proteins in cirrhotic liver. Inducing or accelerating their apoptosis is a potential way of liver fibrosis treatment. Extracellular matrix (ECM) surrounding cells in tissue affects their differentiation, migration, proliferation and function. Type I collagen is the main ECM component in fibrotic liver. We have examined how this protein modifies apoptosis of normal rat HSC induced by gliotoxin, cycloheximide and cytochalasin D in vitro and spontaneous apoptosis of HSC isolated from CCl 4 -damaged liver. We have found that type I collagen gel enhances HSC apoptosis regardless of the agent triggering this process.

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“…RNA isolation and analysis was performed as described previously 23 . Total cellular RNA was isolated from the GT and reverse transcribed.…”

Section: Methodsmentioning

confidence: 99%

Effects of hyaluronan and iodine on wound contraction and granulation tissue formation in rat skin wounds

Slavkovský

Köhlerová²,

Jiroutová³

et al. 2010

Clinical and Experimental Dermatology

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Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts

Cited by 7 publications

References 42 publications

Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver

Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver

Effect of Collagen I Gel on Apoptosis of Rat Hepatic Stellate Cells

Effects of hyaluronan and iodine on wound contraction and granulation tissue formation in rat skin wounds

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