Expression of mt1 melatonin receptor in rat retina: evidence for multiple cell targets for melatonin

Fujieda, Hiroki; Hamadanizadeh, Soheila A.; Wankiewicz, Ellen; Pang, S.F.; Brown, Gregory M.

doi:10.1016/s0306-4522(99)00111-6

Cited by 85 publications

(80 citation statements)

References 50 publications

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“…For the detection of mt 1 melatonin receptor protein, prostate cancer LNCaP and PC-3 cells in logarithmic growth phase were also cultured on glass coverslips. Both tumor tissues and cells were incubated overnight at 4 C with 5 mg/l puri®ed polyclonal rabbit anti-mt 1 receptor serum [11,20,21], kindly supplied by Dr. G.M. Brown (Toronto, Canada).…”

Section: Effects Of Melatonin On Tumor Growth In Athymic Nude Micementioning

confidence: 99%

Inhibition of androgen-sensitive LNCaP prostate cancer growth in vivo by melatonin: Association of antiproliferative action of the pineal hormone with mt1 receptor protein expression

Siu

Fong

et al. 2001

Prostate

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Section: Effects Of Melatonin On Tumor Growth In Athymic Nude Micementioning

confidence: 99%

Inhibition of androgen-sensitive LNCaP prostate cancer growth in vivo by melatonin: Association of antiproliferative action of the pineal hormone with mt1 receptor protein expression

Siu

Fong

et al. 2001

Prostate

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“…Two mammalian receptor subtypes with high affinity for melatonin have been cloned and characterized (Reppert et al 1994(Reppert et al , 1995 and were denominated as MT1 (Mel1a) and MT2 (Mel1b) melatonin receptors. Investigations of the rat retina by use of in situ hybridization have revealed the expression of the MT1 receptor mRNA in ganglion and horizontal cells (Fujieda et al 1999). MT1 receptor immunoreactivity was found in amacrine and ganglion cells of humans and guinea pigs and also in photoreceptor cells of the human retina (Fujieda et al 1999;Scher et al 2002;Savaskan et al 2002).…”

Section: Introductionmentioning

confidence: 98%

Impact of melatonin receptors on pCREB and clock-gene protein levels in the murine retina

Dinet

Korf

2007

Cell Tissue Res

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In several mammalian species, the retina is capable of synthesizing melatonin and contains an autonomous circadian clock that relies on interlocking transcriptional/translational feedback loops involving several clock genes, such as Per1 and Cry2. Our previous investigations have shown remarkable differences in retinae of melatonin-deficient (C57BL) and melatonin-proficient (C3H) mice with regard to the protein levels of PER1, CRY2, and phosphorylated (p) cyclic AMP response element binding protein (CREB). To elucidate the melatonin receptor type possibly responsible for these differences, we have performed immunocytochemical analyses for PER1, CRY2, and pCREB in retinae of melatonin-proficient wild type (WT) mice and mice with targeted deletions of the MT1 receptor (MelaaBB) or the MT1 and MT2 receptors (Melaabb) at four different time points. Immunoreactions for PER1, CRY2 and pCREB were localized to the nuclei of cells in the inner nuclear layer (INL) and ganglion cell layer (GC) of all strains. Surprisingly, in MelaaBB and Melaabb the day/night rhythm of pCREB, PER1, and CRY2 levels was not abolished, but the maxima and minima of PER1 were 180 degrees out of phase as compared to the WT. These data suggest that MT1 and MT2 melatonin receptors are not necessary to maintain rhythmic changes in clock-gene protein levels in the murine retina, but, as shown for PER1, appear to be involved in internal synchronization.

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“…MT 1 and MT 2 receptors are both present in the vertebrate retina (reviewed in: [ 57 ], but see also Table 4.1 ). In rats, MT 1 receptors are found in the horizontal and amacrine cells within the inner plexiform layer, retinal ganglion cells (RGCs), and the retinal pigment epithelium (RPE) [ 58 ]. Dopaminergic neurons may also express MT 1 receptors [ 59 ], suggesting that melatonin can directly modulate the activity of these cells and is consistent with observations, in many species, that melatonin inhibits dopamine release [ 40 , 60 , 61 ].…”

Section: Melatonin: Site Of Action and Signalingmentioning

confidence: 57%

Role of Melatonin and Dopamine in the Regulation of Retinal Circadian Rhythms

Tosini

Iuvone

2013

The Retina and Circadian Rhythms

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Melatonin and dopamine are two important players in the regulation of many physiological functions within the body. These neurohormones are also involved in the regulation of retinal physiology and its pathophysiology. Several studies have shown that melatonin synthesis in the retina primarily occurs during the night, whereas dopamine synthesis occurs during the day. We now know that melatonin and dopamine play opposing roles in the regulation of retinal physiology and visual processing in mammals; dopamine functions as a signal for light adaptation, while melatonin has dark-adaptive effects. This reciprocal feedback loop is believed to be a critical component of the regulation of circadian rhythms in the eye. Melatonin and dopamine exert their infl uence(s) by binding to G-protein-coupled receptors that are expressed by many different cells in the vertebrate retina. Although during the last 20 years we have acquired a substantial knowledge on the role of melatonin and dopamine in many different animal models, a clear role for the melatonin and dopamine systems in human retina is still missing. It is our hope that future genomic studies will be performed on human populations with ocular diseases to determine if polymorphisms in genes encoding enzymes-or receptorsinvolved in the melatonin and dopamine systems are associated with specifi c visual disorders.

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Expression of mt1 melatonin receptor in rat retina: evidence for multiple cell targets for melatonin

Cited by 85 publications

References 50 publications

Inhibition of androgen-sensitive LNCaP prostate cancer growth in vivo by melatonin: Association of antiproliferative action of the pineal hormone with mt1 receptor protein expression

Inhibition of androgen-sensitive LNCaP prostate cancer growth in vivo by melatonin: Association of antiproliferative action of the pineal hormone with mt1 receptor protein expression

Impact of melatonin receptors on pCREB and clock-gene protein levels in the murine retina

Role of Melatonin and Dopamine in the Regulation of Retinal Circadian Rhythms

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