Expression of Mucin1 in saliva in oral squamous cell carcinoma and oral potentially malignant disorders (case control study)

Abstract

Objectives Overexpression of mucin1 is found in head and neck squamous cell carcinoma tissues compared with adjacent non‐neoplastic tissues and higher levels are associated with metastasis and invasion. The expression level of mucin1 in saliva of normal individuals, oral potentially malignant disorders and oral squamous cell carcinoma patients and its correlation to clinical and histological variables was evaluated. Subjects and Methods Forty oral potentially malignant disorders, 40 oral squamous cell carcinoma subjects, and 20 age matched‐controls were included. Stimulated salivary samples were collected from all participants, and mucin1 expression was measured by real‐time PCR. Results Mucin1 expression in saliva was significantly elevated in oral potentially malignant disorders when compared with controls. Similarly, mucin1 expression was significantly elevated in oral squamous cell carcinoma group when compared with oral potentially malignant disorders and controls. Mucin1 expression in OSCC patient showed significant positive correlations with T classification and distant Metastasis. Mucin1 expression in oral potentially malignant disorders patients showed significant positive correlations with degree of dysplasia. Conclusions The expression level of mucin1 in saliva might be a potential biomarker for diagnosing oral potentially malignant disorders and oral squamous cell carcinoma.

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

“…Mucin genes such as muc1 and muc4 are expressed broadly in epithelial cells of the body, including the upper aero-respiratory tract and oral cavity [40]. Over-and under-expression of mucin genes and other modifications, such as aberrant glycosylation, have been implicated in situations of epithelial dysfunction, including malignant transformation [41,42] and breakdown of the nasal epithelial barrier [43]. For the OKF6/TERT-2 cell model to be representative of in vivo physiology, the production of mucins should remain consistent.…”

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

“…Mucin genes such as muc1 and muc4 are expressed broadly in epithelial cells of the body, including the upper aero-respiratory tract and oral cavity [43]. Over-and under-expression of mucin genes and other modifications, such as aberrant glycosylation, have been implicated in situations of epithelial dysfunction, including malignant transformation [44,45] and breakdown of the nasal epithelial barrier [46]. For the OKF6/TERT-2 cell model to be representative of in vivo physiology, the production of mucins should remain consistent.…”

MUC4 expression in oral dysplastic epithelium and oral squamous cell carcinoma: An immunohistochemical study

“…It indicates a higher risk of malignant transformation, after which a grade of severity is assigned. [ 1 2 3 ] There is a strong connection between more severe grades of dysplasia and an elevated likelihood of developing cancer. [ 4 5 6 ] These kinds of lesions are considered to be potentially malignant conditions because of the intrinsic disparity that exists between them.…”