Expression of Mucins in Mucoid Otitis Media

Lin, Jizhen; Tsuboi, Yasuhiro; Rimell, Frank L.; Liu, George; Toyama, Katsuhiro; Kawano, Hirokazu; Paparella, Michael M.; Ho, Samuel B.

doi:10.1007/s10162-002-3023-9

Cited by 50 publications

(57 citation statements)

References 36 publications

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“…Our laboratory and others have detailed the importance of mucin proteins with gel-forming qualities, MUC2 [3], MUC5AC [14–15], MUC5B [16–18] , and the more recently described MUC19 [19], given that these proteins are secreted. These secretory properties allow these mucins to function at the apical surface of the epithelium and beyond as they interact with pathogens and protect the underlying epithelium from invasion and mechanical damage.…”

Section: Discussionmentioning

confidence: 99%

Mucin gene expression and mouse middle ear epithelium

Kerschner

Tsushiya

et al. 2010

International Journal of Pediatric Otorhinolaryngology

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Objectives To investigate the expression of recently identified human mucin genes in an in vitro model of cultured mouse middle ear epithelial cells (MMEEC). Methods MMEEC were established, RNA was extracted and primers were designed for RT-PCR to assess for expression of mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc6, Muc7, Muc8, Muc9, Muc10, Muc11/12, Muc13, Muc15, Muc16, Muc17, Muc18, Muc19 and Muc20 expression. Results Mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc9, Muc10, Muc13, Muc15, Muc16, Muc18, Muc19 and Muc20 were identified and expressed in MMEEC. The genes Muc6, Muc7, Muc8, Muc11/12 and Muc17 were not identified. Conclusion Many of the mucin genes that have been recently identified in human MEE and chinchilla MEE are also expressed in MMEEC. There are differences in expression, however, which may have implications in utilizing various animal models for study of middle ear physiology and pathogenesis; specifically as it relates to mucin gene expression.

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Section: Discussionmentioning

confidence: 99%

Mucin gene expression and mouse middle ear epithelium

Kerschner

Tsushiya

et al. 2010

International Journal of Pediatric Otorhinolaryngology

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“…Currently, investigations on specific mucins and their roles in diseases, mucosal defense, and epithelial repair/remodeling are underway in many laboratories. Mucins implicated in cancer (110), lung diseases (153,286), gastrointestinal diseases (53,70), as well as mucins expressed in the eye (90) and ear (164), have recently been reviewed.…”

Section: A Overviewmentioning

confidence: 99%

Respiratory Tract Mucin Genes and Mucin Glycoproteins in Health and Disease

Rose

Voynow²

2006

Physiological Reviews

956

892

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This review focuses on the role and regulation of mucin glycoproteins (mucins) in airway health and disease. Mucins are highly glycosylated macromolecules (≥50% carbohydrate, wt/wt). MUC protein backbones are characterized by numerous tandem repeats that contain proline and are high in serine and/or threonine residues, the sites of O-glycosylation. Secretory and membrane-tethered mucins contribute to mucociliary defense, an innate immune defense system that protects the airways against pathogens and environmental toxins. Inflammatory/immune response mediators and the overproduction of mucus characterize chronic airway diseases: asthma, chronic obstructive pulmonary diseases (COPD), or cystic fibrosis (CF). Specific inflammatory/immune response mediators can activate mucin gene regulation and airway remodeling, including goblet cell hyperplasia (GCH). These processes sustain airway mucin overproduction and contribute to airway obstruction by mucus and therefore to the high morbidity and mortality associated with these diseases. Importantly, mucin overproduction and GCH, although linked, are not synonymous and may follow from different signaling and gene regulatory pathways. In section i, structure, expression, and localization of the 18 human MUC genes and MUC gene products having tandem repeat domains and the specificity and application of MUC-specific antibodies that identify mucin gene products in airway tissues, cells, and secretions are overviewed. Mucin overproduction in chronic airway diseases and secretory cell metaplasia in animal model systems are reviewed in section ii and addressed in disease-specific subsections on asthma, COPD, and CF. Information on regulation of mucin genes by inflammatory/immune response mediators is summarized in section iii. In section iv, deficiencies in understanding the functional roles of mucins at the molecular level are identified as areas for further investigations that will impact on airway health and disease. The underlying premise is that understanding the pathways and processes that lead to mucus overproduction in specific airway diseases will allow circumvention or amelioration of these processes.

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“…It is clear in the recent studies that MUC5B is the predominant one [12,13,29,30,32]. MUC5B is actually a mucin expressed in submucosal glands, instead of in the surface epithelia, but it is highly upregulated in COM [29,31,32].…”

Section: Mucin Dysregulation In Middle Earmentioning

confidence: 96%

“…MUC5B mucin is a polymer, linked head-tohead. Under a microscope, mucous strings can be found [12,13,29,32]. That is a molecular basis for trapping invading microorganisms.…”

Section: Mucin Dysregulation In Middle Earmentioning

confidence: 98%