Expression of Mug14 is regulated by the transcription factor Rst2 through the cAMP-dependent protein kinase pathway in Schizosaccharomyces pombe

Inamura, Shin-Ich; Tanabe, Takuma; Kawamukai, Makoto; Matsuo, Yasuhiro

doi:10.1007/s00294-021-01194-z

Cited by 8 publications

(6 citation statements)

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“…The transcription factor Rst2 is negatively regulated by Pka1 (Higuchi et al 2002, Inamura et al 2021. Our observation of rst2∆ combined with various other deletion mutants indicate that Rst2 is important for KCl tolerance.…”

Section: Discussionmentioning

confidence: 63%

“…The TOR pathway is activated in response to nutrient starvation, such as nitrogen or glucose limitation (Halova et al 2013, Saitoh et al 2015. The cAMP/PKA pathway mainly responds to glucose concentration (Hoffman 2005, Inamura et al 2021, Welton and Hoffman 2000.…”

Section: Introductionmentioning

confidence: 99%

“…S1). Through the promoter region of the stress starvation response element of S. pombe (STREP: CCCCTC), Rst2 regulates the expression of ste11, which encodes a transcription factor to regulate sexual development (Sugimoto et al 1991); fbp1, which encodes fructose-1,6-bisphosphatase (Hoffman and Winston 1990); and mug14, which encodes an adducin homolog (Inamura et al 2021). The cAMP/PKA pathway involves many cellular events, including transition to meiosis (Maeda et al 1994, Matsuo et al 2008, Wu and McLeod 1995, chronological aging (Roux et al 2006), stress response (Matsuo and Kawamukai 2017, Matsuo et al 2008, Stiefel et al 2004), glucose sensing (Byrne andHoffman 1993, Inamura et al 2021), proper chromosome segregation (Tanabe et al 2019), and proper microtubule structure maintenance (Tanabe et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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Synergistic roles of the phospholipase B homolog Plb1 and the cAMP-dependent protein kinase Pka1 in the hypertonic stress response of Schizosaccharomyces pombe

2022

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The phospholipase B homolog Plb1 and the cAMP-dependent protein kinase (PKA) pathway are required by ssion yeast, also known as to Schizosaccharomyces pombe, to grow under KCl stress conditions. Here, we report the relative contributions of Plb1 and the cAMP/PKA pathway during the hypertonic stress response. We show that the plb1∆, cyr1∆, and pka1∆ single mutants are sensitive to high concentrations of KCl but insensitive to sorbitol-induced osmotic stress. In contrast, the plb1∆ cyr1∆ and plb1∆ pka1∆ double mutants are hypersensitive to KCl and sorbitol. The cyr1∆ pka1∆ double mutants showed the same phenotype of each single mutant. Growth inhibition due to hypertonic stress in the plb1∆, plb1∆ cyr1∆, and plb1∆ pka1∆ strains was partially rescued by cgs1 deletion-cgs1∆ has constitutively active Pka1-or by the deletion of transcription factor Rst2, which is negatively regulated by Pka1. Pka1-GFP localized in the nucleus and cytoplasm in plb1∆, whereas it localized only in the cytoplasm in cyr1∆, indicating that Plb1 does not regulate Pka1 localization. Glucose limitation downregulates the PKA pathway, and it was accordingly observed that glucose limitation in plb1∆ further increased the strain's sensitivity to KCl. Growth inhibition by KCl in plb1∆ under glucose-limited conditions was signi cantly rescued by cgs1∆ and slightly rescued by rst2∆. These ndings indicate that, in ssion yeast, the glucose-sensing cAMP/PKA pathway is mainly responsible for responding to stressful hypertonic conditions induced by high levels of KCl, and that Plb1 has a subsidiary role in this stress response.

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Section: Discussionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synergistic roles of the phospholipase B homolog Plb1 and the cAMP-dependent protein kinase Pka1 in the hypertonic stress response of Schizosaccharomyces pombe

2022

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“…Activated Pka1 regulates target proteins, including transcription factor Rst2. Rst2 is negatively regulated via phosphorylation by Pka1 (Higuchi et al 2002;Inamura et al 2021). The cAMP/PKA pathway also plays a role in the transition to meiosis (Maeda et al 1994 In this study, we identi ed rst2∆, tfs1∆, mca1∆, and moc3∆ as suppressors of TBZ-sensitive pka1∆ strains by screening the double-deletion strain of pka1 and 102 individual genes encoding transcriptionrelated factors in the S. pombe.…”

Section: Introductionmentioning

confidence: 99%

Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe

et al. 2023

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The cAMP-dependent protein kinase (PKA) pathway in Schizosaccharomyces pombe plays an important role in microtubule organization and chromosome segregation. Typically, loss of functional Pka1 induces sensitivity to the microtubule-destabilizing drug thiabendazole (TBZ) and chromosome mis-segregation.To determine the mechanism via which Pka1 is involved in these events, we explored the relevance of transcription factors by creating a double-deletion strain of pka1 and 102 individual genes encoding transcription factors. We found that rst2∆, tfs1∆, mca1∆, and moc3∆ suppressed the TBZ-sensitive phenotype of the pka1∆ strain, among which tfs1∆ was the strongest suppressor. All single mutants (rst2∆, tfs1∆, mca1∆, and moc3∆) showed a TBZ-tolerant phenotype. Tfs1 has two transcriptional domains (TFIIS and Zn nger domains), both of which contributed to the suppression of the pka1∆induced TBZ-sensitive phenotype. pka1∆-induced chromosome mis-segregation was rescued by tfs1∆ in the presence of TBZ. tfs1 overexpression induced the TBZ-sensitive phenotype and a high frequency of chromosome mis-segregation, suggesting that the amount of Tfs1 must be strictly controlled. However, Tfs1-expression levels did not differ between the wild-type and pka1∆ strains, and the Tfs1-GFP protein was localized to the nucleus and cytoplasm in both strains, which excludes the direct regulation of expression and localization of Tfs1 by Pka1. Growth inhibition by TBZ in pka1∆ strains was notably rescued by double deletion of rst2 and tfs1 rather than single deletion of rst2 or tfs1, indicating that Rst2 and Tfs1 contribute independently to counteract TBZ toxicity. Our ndings highlight Tfs1 as a key transcription factor for proper chromosome segregation.

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“…Only have a handful of fungal ZFPs been studied in the past decade. In Schizosaccharomyces pombe , Prz1 functions downstream of calcineurin (Koike et al, 2012), and Loz1 and Rst2 mediate zinc homeostasis (Corkins et al, 2013) and transition from mitosis to meiosis (Inamura et al, 2021), respectively. AslA acts as a TF of vacuolar homeostasis‐related genes in Aspergillus nidulans (Park et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Characterization of BbKlf1 as a novel transcription factor vital for asexual and infection cycles of Beauveria bassiana

Mohamed

et al. 2022

Environ Microbiol Rep

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The large family of C2H2‐type zinc finger transcription factors (TFs) comprise the Kruppel‐like factors (KLFs) that evolved relatively late in eukaryotes but remain unexplored in filamentous fungi. Here, we report that an orthologue (BbKlf1) of yeast Klf1 mediating cell wall integrity (CWI) is a wide‐spectrum TF evidently localized in nucleus and cytoplasm in Beauveria bassiana. BbKlf1 features conserved domains and multiple DNA‐binding motifs predicted to bind multiple promoter DNA fragments of target genes across asexual developmental and stress‐responsive pathways. Despite limited impact on normal colony growth, deletion of Bbklf1 resulted in impaired CWI and hypersensitivity to Congo red‐induced cell wall stress. Also, the deletion mutant was severely compromised in tolerance to oxidative and osmotic stresses, hyphal septation and differentiation, conidiation capacity (reduced by 95%), conidial quality (viability and hydrocarbon epitope pattern) and virulence. Importantly, these phenotypes correlated well with sharply repressed or nearly abolished expressions of those genes required for or involved in chitin biosynthesis, antioxidant activity, cell division and differentiation, aerial conidiation and conidial maturation. These findings indicate an essentiality of BbKlf1 for the asexual and insect‐pathogenic lifecycles of B. bassiana and a novel scenario much beyond the yeast orthologue‐mediated CWI, suggesting important roles of its orthologues in filamentous fungi.

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Expression of Mug14 is regulated by the transcription factor Rst2 through the cAMP-dependent protein kinase pathway in Schizosaccharomyces pombe

Cited by 8 publications

References 60 publications

Synergistic roles of the phospholipase B homolog Plb1 and the cAMP-dependent protein kinase Pka1 in the hypertonic stress response of Schizosaccharomyces pombe

Synergistic roles of the phospholipase B homolog Plb1 and the cAMP-dependent protein kinase Pka1 in the hypertonic stress response of Schizosaccharomyces pombe

Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe

Characterization of BbKlf1 as a novel transcription factor vital for asexual and infection cycles of Beauveria bassiana

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