Expression of myogenic regulatory factors during the development of mouse tongue striated muscle

Yamane, Akira; Mayo, Mark L.; Shuler, C. F.; Crowe, David L.; Okita, Yoshiki; Dalrymple, Kirsten; Saeki, Yasutake

doi:10.1016/s0003-9969(99)00105-3

Cited by 64 publications

(70 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total RNA extraction, treatment with deoxyribonuclease I, and reverse transcription were performed as previously described Yamane et al, 2000). Briefly, total RNA was isolated from individual soleus muscles according to the manufacturer's specifications (Trizol, Life Technologies, Gaithersburg, MD).…”

Section: Quantitative Rt-pcrmentioning

confidence: 99%

Changes in Triacylglycerol‐Accumulated Fiber Type, Fiber Type Composition, and Biogenesis in the Mitochondria of the Soleus Muscle in Obese Rats

Kaneko

Iida

Suga

et al. 2011

The Anatomical Record

View full text Add to dashboard Cite

Little is known about the effects of obesity on skeletal muscle consisting of approximately 80% type I (slow) fibers, such as that in the soleus muscle, although type I fibers have an enhanced capacity for mitochondrial respiration and fatty acid oxidation. We investigated the effects of obesity on the soleus muscle in the rat. Rats were fed a high-fat diet (protein:fat:-carbohydrate ¼ 20:57:23; 508 kcal/100 g) or a control diet (protein:fat:carbohydrate ¼ 20:10:70; 366 kcal/100 g) for 10 weeks. We analyzed the accumulation of intramyocellular triacylglycerol (IMTG), fiber type composition, and the biogenesis and function of the mitochondria in the soleus muscle of the rat during 10 weeks of feeding, using histochemical and realtime polymerase chain reaction analyses. Obesity increased body weight and markedly elevated IMTG levels in type I, but not in type II, fibers of the soleus muscle throughout the feeding period. Obesity also inhibited the biogenesis and function in the mitochondria and altered the fiber type composition in the soleus muscle. The suppression of biogenesis and function in the mitochondria, and the alteration in the fiber type composition may be attributable to the marked IMTG accumulation in the soleus muscle of the rat. Anat Rec, 294:1904Rec, 294: -1912Rec, 294: , 2011. V V C 2011 Wiley-Liss, Inc.

show abstract

Section: Quantitative Rt-pcrmentioning

confidence: 99%

Changes in Triacylglycerol‐Accumulated Fiber Type, Fiber Type Composition, and Biogenesis in the Mitochondria of the Soleus Muscle in Obese Rats

Kaneko

Iida

Suga

et al. 2011

The Anatomical Record

View full text Add to dashboard Cite

show abstract

“…Desmin, Mck, and Tnnc are three marker genes for identifying the states of myofiber development. Yamane et al (2000) detected the developmental expression profiles of these three maker genes in the tongue and limb muscle tissues in mice and compared the developmental differences of the two muscle tissues (Yamane et al 2000). In our research, the developmental expression profiles of these three maker genes have been detected in the in vitro cultured duck myoblasts at different stages.…”

Section: Discussionmentioning

confidence: 75%

“…Among them, Desmin will be highly expressed when myoblasts reentering into the cell cycle, Mck will be highly expressed when the multinucleotide myotubes begin differentiating and Tnnc will be expressed during myofiber formation and maturation (Yamane et al 2000). However, the developmental states of in vitro cultured myoblasts are usually distinguished by their external characteristics, which will hardly give an exact result.…”

Section: Introductionmentioning

confidence: 99%

Characterization of in vitro cultured myoblasts isolated from duck (Anas platyrhynchos) embryo

Liu

Chen

et al. 2011

Cytotechnology

View full text Add to dashboard Cite

Myoblasts isolated from duck embryonic muscle were purified and in vitro cultured. External characteristics were observed by using the immunofluorescence technique, and growth curve of duck embryonic myoblasts was established after measuring with the MTT method. Moreover, mRNA expression of three marker genes, the Desmin, the muscle creatine kinase (Mck) and the troponin C (Tnnc), which could reflect the development status of myofibers, were detected each 24 h for cultured cells by using the qPCR technique. Results showed that the in vitro cultured duck myoblasts went through a series of developmental stages, including the proliferation of myoblasts, the differentiation of multi-nuclei myotubes, and the formation of myofiber. The cultured duck embryonic myoblasts entered into a logarithmic stage approximately on the fourth day after seeding. Accompanying with its progressive growth before entering into the logarithmic phase, the myoblasts also showed some differentiation phenomena, reflected by a low expression level of Desmin and high expression level of the Mck and Tnnc genes. During the rapid growth of the logarithmic phase, there was a high expression of the Desmin gene, and a low expression level of the Mck gene and the Tnnc gene in the cultured myoblasts. The expression profiles of the three marker genes for muscle development could be used for distinguishing the different developmental stages of in vitro cultured myoblasts at the molecular level, which would be more accurate and more feasible than observing the external characteristics of the cultured cells.

show abstract

“…Total RNA extraction, treatment with DNase I and reverse transcription were performed as described previously (27). SYBR Green real-time PCR was performed using the Thermal Cycler Dice Real Time System (Takara Bio Inc.) with the following cycling parameters: denaturation at 95ºC for 10 min, followed by 40 cycles of denaturation 95ºC for 15 s, and annealing and extension at 55ºC for 15 s. The quantities of target mRNAs were normalized by the quantity of murine glyceraldehyde-3-phosphate dehydrogenase (Gapdh) mRNA.…”

Section: Analysis Of the Target Sequences For Mir-29smentioning

confidence: 99%

<i>MyoD</i> is regulated by the miR-29a-<i>Tet1</i> pathway in C2C12 myoblast cells

Chikenji

Ando

Nariyama

et al. 2016

Journal of Oral Science

View full text Add to dashboard Cite

Expression of myogenic regulatory factors during the development of mouse tongue striated muscle

Cited by 64 publications

References 39 publications

Changes in Triacylglycerol‐Accumulated Fiber Type, Fiber Type Composition, and Biogenesis in the Mitochondria of the Soleus Muscle in Obese Rats

Changes in Triacylglycerol‐Accumulated Fiber Type, Fiber Type Composition, and Biogenesis in the Mitochondria of the Soleus Muscle in Obese Rats

Characterization of in vitro cultured myoblasts isolated from duck (Anas platyrhynchos) embryo

<i>MyoD</i> is regulated by the miR-29a-<i>Tet1</i> pathway in C2C12 myoblast cells

Contact Info

Product

Resources

About