Expression of N-CAM polypeptides in neurons

Nybroe, Ole; Gibson, Ann; Rohde, Hanne; Dahlin, Jan; Bock, Elisabeth

doi:10.1016/0197-0186(86)90147-6

Cited by 13 publications

(6 citation statements)

References 23 publications

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“…Thus, in adult lung the lack of NCAM reactive deposits on the peripheral regions of NEB cells might indicate that their presence as a functional entity has become less important. The pulmonary neu-ral staining profile of NCAM throughout all life stages studied (Seldeslagh and Lauweryns, 1993a,b) confirms earlier findings, which stated that NCAM is still present on nerve fibers and ganglion cells in the adult though at much reduced levels and may also be associated with a slower turnover (Crossin et al, 1985;Langley and Gratzl, 1990;Nybroe et al, 1986).…”

Section: Discussionsupporting

confidence: 86%

NCAM expression in the pulmonary neural and diffuse neuroendocrine cell system

Seldeslagh

Lauweryns

1997

Microsc. Res. Tech.

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Neural cell adhesion molecule (NCAM)—a membrane protein involved in a cell‐cell adhesion within the central and peripheral nervous systems—was demonstrated to be a sensitive and specific marker for neuroepithelial bodies (NEB) and neural tissue elements in the cat lung. Using the streptavidin‐biotin immunoperoxidase method, NCAM reactive sites were investigated with monoclonal and polyclonal antibodies on serial sections of Bouin fixed, paraffin embedded lung tissue. Moreover, NCAM expression was compared with that of neuron‐specific enolase (NSE) on adjacent sections. The most obvious NCAM staining was obtained with the monoclonal antibody. From newborn to adult life, cell surface labeling was identified on NEB cells. In mature cat lung, they were no longer positive. Solitary neuroendocrine cells (NEC) were always negative. In contrast to the transient postnatal immunoreactivity of NEB cells, nerve fibers and ganglion cells were stained throughout all life stages studied. The distribution of NCAM in NEB, nerve fibers and ganglion cells was similar to that of NSE, except in the adult lung. This study reveals that during lung growth shared NCAM antigens exist between the pulmonary nervous and endocrine system, whereas in mature lung NCAM proteins are confined to neural tissue elements. The difference in NCAM expression between NEB and NEC might suggest an involvement of NCAM in the formation of contacts between NEB cells and nerves. Microsc. Res. Tech. 37:69–76, 1997. © 1997 Wiley‐Liss, Inc.

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Section: Discussionsupporting

confidence: 86%

NCAM expression in the pulmonary neural and diffuse neuroendocrine cell system

Seldeslagh

Lauweryns

1997

Microsc. Res. Tech.

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“…This observation is not surprising, given that NCAMΔCT could be considered to be a functional and structural equivalent of the NCAM120 isoform of NCAM, which is anchored to the membrane through GPI and lacks an intracellular domain and, hence, is transported by mechanisms distinct from those of NCAM180 and NCAM140 (Garner et al, 1986;Nybroe et al, 1986). Our observation that cell surface levels of NCAMΔCT are similar to or even higher than those for NCAM180 indicates that KLC1 is not required for cell surface delivery of NCAM, but rather suggests that this interaction plays a role in regulation of NCAM transport.…”

Section: Discussionmentioning

confidence: 99%

“…In axons, NCAM is distributed by fast axonal transport (Garner et al, 1986;Nybroe et al, 1986). In dendrites and glial cell processes, NCAM also needs to be distributed to the cell surface after its transport to the sites of insertion.…”

Section: Introductionmentioning

confidence: 99%

Kinesin-1 promotes post-Golgi trafficking of NCAM140 and NCAM180 to the cell surface

Wobst

Schmitz

Schachner

et al. 2015

Journal of Cell Science

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The neural cell adhesion molecule (NCAM, also known as NCAM1) is important during neural development, because it contributes to neurite outgrowth in response to its ligands at the cell surface. In the adult brain, NCAM is involved in regulating synaptic plasticity. The molecular mechanisms underlying delivery of NCAM to the neuronal cell surface remain poorly understood. We used a protein macroarray and identified the kinesin light chain 1 (KLC1), a component of the kinesin-1 motor protein, as a binding partner of the intracellular domains of the two transmembrane isoforms of NCAM, NCAM140 and NCAM180. KLC1 binds to amino acids CGKAGPGA within the intracellular domain of NCAM and colocalizes with kinesin-1 in the Golgi compartment. Delivery of NCAM180 to the cell surface is increased in CHO cells and neurons co-transfected with kinesin-1. We further demonstrate that the p21-activated kinase 1 (PAK1) competes with KLC1 for binding to the intracellular domain of NCAM and contributes to the regulation of the membrane insertion of NCAM. Our results indicate that NCAM is delivered to the cell surface through a kinesin-1-mediated transport mechanism in a PAK1-dependent manner.

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“…NCAM, which has been reported to be expressed by both neurons and glia (Noble et al, 1985;Nybroe et al, 1986;Persohn and Schachner, 1990), was expressed on the surface of astrocytes in both control and NGF-producing fibroblast grafts. NCAM was also found to be colocalized with neurofilament-IR axons in NGF-producing fibroblast grafts.…”

Section: Ngf-producingmentioning

confidence: 97%

Sprouting adult CNS cholinergic axons express nile and associate with astrocytic surfaces expressing neural cell adhesion molecule

1996

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To assess the cellular and molecular substrates for cholinergic axon growth in the adult central nervous system (CNS), we implanted grafts of control and nerve growth factor (NGF)-producing genetically modified fibroblasts within the striatum of rats. Sprouting cholinergic axonal processes that grew into grafts of NGF-producing fibroblasts were fasciculated and followed the surface of astrocytic processes for long distances within the grafts. The close and long distance anatomical relationship between the sprouted axons and the astrocytes supported previous ultrastructural evidence that astrocytes may serve as a cellular substrate for sprouting cholinergic axons in vivo. The sprouted axon processes were associated with the expression of nerve growth factor-inducible large external (NILE) glycoprotein on their surfaces. NILE expression was not seen in control grafts where there was an absence of cholinergic ingrowth. NILE has been demonstrated to play a role in axon fasciculation in a number of other neural systems. The astrocytic processes in both control and NGF-producing fibroblast grafts expressed neural cell adhesion molecule (NCAM), suggesting that NCAM-mediated adhesion may be responsible for the close relationship between the axons and astrocytes within the grafts. NGF-induced heterotypic interactions between neuronal NILE and astroglial NCAM may also be required for adult cholinergic axonal sprouting.

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Expression of N-CAM polypeptides in neurons

Cited by 13 publications

References 23 publications

NCAM expression in the pulmonary neural and diffuse neuroendocrine cell system

NCAM expression in the pulmonary neural and diffuse neuroendocrine cell system

Kinesin-1 promotes post-Golgi trafficking of NCAM140 and NCAM180 to the cell surface

Sprouting adult CNS cholinergic axons express nile and associate with astrocytic surfaces expressing neural cell adhesion molecule

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