Expression of NADPH oxidase is induced by all-trans retinoic acid but not by phorbol myristate acetate and 1,25 dihydroxyvitamin D3 in the human promyelocytic cell line NB4

N’Diaye, Elsa-Noah; Vaissiere, C.; González-Christen, Judith; Gregoire, C.; Cabec, Véronique Le; Maridonneau‐Parini, Isabelle

doi:10.1038/sj.leu.2400855

Cited by 15 publications

(12 citation statements)

References 14 publications

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“…Although other pathways cannot be ruled out, this was similar to the previously reported induction of ROS by RA (N'Diaye et al, 1997). Recently a study showed that VD alone can induce autophagosome formation to kill internalized Mtb (Yuk et al, 2009).…”

Section: Discussionsupporting

confidence: 91%

A Novel in vitro Human Macrophage Model to Study the Persistence of Mycobacterium tuberculosis Using Vitamin D3 and Retinoic Acid Activated THP-1 Macrophages

Estrella¹,

Kan-Sutton²,

Gong³

et al. 2011

Front. Microbio.

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Mycobacterium tuberculosis (Mtb) replicates within the human macrophages and we investigated the activating effects of retinoic acid (RA) and vitamin D3 (VD) on macrophages in relation to the viability of intracellular Mtb. A combination of these vitamins (RAVD) enhanced the levels of DC-SIGN and mannose receptors on THP-1 macrophages that increased mycobacterial uptake but inhibited the subsequent intracellular growth of Mtb by inducing reactive oxygen species and autophagy. RAVD also enhanced antigen presenting and chemotactic receptors on THPs suggesting an activated phenotype for RAVD activated THPs. RAVD mediated activation was also associated with a marked phenotypic change in Mtb infected THPs that fused with adjacent THPs to form multinucleated giant cells (MNGCs). Typically, MNGCs occurred over 30 days of in vitro culture and contained non-replicating persisting Mtb for more than 60 days in culture. Latent tuberculosis occurs in over a third of mankind and we propose that RAVD mediated induction of persistent Mtb within human macrophages provides a novel model to develop therapeutic approaches and investigate pathogenesis of latency.

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Section: Discussionsupporting

confidence: 91%

A Novel in vitro Human Macrophage Model to Study the Persistence of Mycobacterium tuberculosis Using Vitamin D3 and Retinoic Acid Activated THP-1 Macrophages

Estrella¹,

Kan-Sutton²,

Gong³

et al. 2011

Front. Microbio.

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“…It has been reported that NADPH oxidase subunits are not expressed in promyelocytic leukemia cells (N'Diaye et al, 1997). Nevertheless, we presented evidence suggesting that NB4 and U937 cells express all NADPH oxidase subunits, but the expression profile could not explain the higher activity of NADPH oxidase in NB4 cells compared with that in U937 cells.…”

Section: Discussioncontrasting

confidence: 47%

PML/RARα fusion protein mediates the unique sensitivity to arsenic cytotoxicity in acute promyelocytic leukemia cells: Mechanisms involve the impairment of cAMP signaling and the aberrant regulation of NADPH oxidase

Wang

et al. 2008

Journal Cellular Physiology

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Acute promyelocytic leukemia (APL) cells are characterized by PML/RARalpha fusion protein, high responsiveness to arsenic trioxide (ATO)-induced cytotoxicity and an abundant generation of reactive oxygen species (ROS). In this study we investigated the association among these three features in APL-derived NB4 cells. We found that NADPH oxidase-derived ROS generation was more abundant in NB4 cells compared with monocytic leukemia U937 cells. By using PR9, a sub-line of U937 stably transduced with the inducible PML/RARalpha expression vectors, we attributed disparities on ROS generation and ATO sensitivity to the occurrence of PML/RARalpha fusion protein, since PML/RARalpha-expressing cells appeared higher NADPH oxidase activity, higher ROS level and higher sensitivity to ATO. On the other hand, the basal intensity of cAMP signaling pathway was compared between NB4 and U937 as well as between PR9 cells with or without PML/RARalpha, demonstrating that PML/RARalpha-expressing cells had an impaired cAMP signaling pathway which relieved its inhibitory effect on NADPH oxidase derived ROS generation. In summary, the present study demonstrated the correlation of PML/RARalpha with cAMP signaling pathway, NADPH oxidase and ROS generation in APL cells. PML/RARalpha that bestows NB4 cells various pathological features, paradoxically also endows these cells with the basis for susceptibility to ATO-induced cytotoxcity.

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“…Next [25]. These cells contain azurophil but not specific granules and cytochrome b 559 is only detected in the plasma membrane fraction [26].…”

Section: Production Of Omentioning

confidence: 99%

NADPH oxidase is functionally assembled in specific granules during activation of human neutrophils

Vaissiere

Cabec

Maridonneau‐Parini

1999

Journal of Leukocyte Biology

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In addition to the extracellular production of O 2؊ by NADPH oxidase in neutrophils stimulated by soluble stimuli, the intracellular formation of oxygen reactive species has been described. Cytochrome b 559 , the redox component of the NADPH oxidase complex, is mainly associated with specific granule membrane in resting neutrophils. We examined whether these granules could be a site for intracellular production of O 2 ؊ . Phorbol myristate acetate (PMA)-stimulated neutrophils were fractionated by differential centrifugation, and generation of O 2 ؊ was detected in both the granule and the plasma membrane-enriched fractions, but more in the granules. Translocation of p47 phox and p67 phox , two cytosolic components of the NADPH oxidase, was also quantitatively more important in the granules than in the plasma membrane fraction. After separation of the specific from the azurophil granules, p47 phox and p67 phox were found to be present only in the specific granules of PMA-activated cells. As a control, the production of O 2 ؊ was studied in retinoic aciddifferentiated NB4 cells that lack specific granules. During stimulation of NB4 cells with PMA, only the plasma membrane-enriched fraction was the site of O 2 ؊ production. Together, these results indicate that NADPH oxidase can be functionally assembled in specific granules. J. Leukoc. Biol. 65: 629-634; 1999.

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Expression of NADPH oxidase is induced by all-trans retinoic acid but not by phorbol myristate acetate and 1,25 dihydroxyvitamin D3 in the human promyelocytic cell line NB4

Cited by 15 publications

References 14 publications

A Novel in vitro Human Macrophage Model to Study the Persistence of Mycobacterium tuberculosis Using Vitamin D3 and Retinoic Acid Activated THP-1 Macrophages

A Novel in vitro Human Macrophage Model to Study the Persistence of Mycobacterium tuberculosis Using Vitamin D3 and Retinoic Acid Activated THP-1 Macrophages

PML/RARα fusion protein mediates the unique sensitivity to arsenic cytotoxicity in acute promyelocytic leukemia cells: Mechanisms involve the impairment of cAMP signaling and the aberrant regulation of NADPH oxidase

NADPH oxidase is functionally assembled in specific granules during activation of human neutrophils

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