Expression of Neuron‐specific Enolase Immunoreactivity by Cutaneous and Extracutaneous Langerhans‐cell Histiocytoses (“X”)

In the course of a molecular genetic investigation of a double inhumation, presumably a mother/child burial from Aschheim (Upper Bavaria, 6th century A.D.), which included analysis of mitochondrial DNA, molecular sexing, and polymorphic nuclear DNA, Yersinia pestis‐specific DNA was detected. Molecular analyses were performed on DNA extracts obtained from two teeth of one skeleton and four teeth of the other. The use of the primer pair YP12D/YP11R (Raoult et al. [2000] Proc. Natl. Acad. Sci. 97:12800–12803), able to amplify part of the Y. pestis plasmid pPCP1 pla sequence, resulted in amplification products of the expected fragment size. Using BLASTN 2.2.2, the sequences of these amplification products shared 100% identity with that of the modern Y. pestis pla sequence in GenBank, with the exception of one amplification product which revealed a single base substitution. The application of a “suicide PCR” with the independent primer pair YP11D/YP10R (Raoult et al. [2000] Proc. Natl. Acad. Sci. 97:12800–12803) resulted in amplification products which shared a 96–98% homology with that of the modern Y. pestis pla sequence in GenBank. The observed deviations were presumably due to miscoding lesions in the template DNA. No modern Y. pestis DNA was introduced into the institute, and thus no positive controls were carried along. All extraction and PCR controls remained negative. The identification of Y. pestis‐specific DNA sequences in these two skeletons, buried in the second half of the 6th century A.D., constitutes molecularly supported evidence for the presence of Y. pestis, the causative agent of plague, during the first pandemic recorded. Am J Phys Anthropol, 2004. © 2004 Wiley‐Liss, Inc.

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Functional imaging of Langerhans cell histiocytosis by ¹¹¹In‐DTPA‐D‐Phe¹‐octreotide scintigraphy

Lastoria

Montella

Catalano

et al. 2002

Cancer

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Langerhans’ cell histiocytosis in adults

Mejía

Dano

Roberts

et al. 1997

Journal of the American Academy of Dermatology

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Proliferative activity and apoptosis of Langerhans histiocytes in eosinophilic granulomas as evaluated by MIB-1 and TUNEL methods

Hamada

Iwaki

Fukui

et al. 1995

Molecular Pathology

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Ains-To identify the role played by apoptosis in tumour regression. Methods-The growth fraction and apoptotic cell loss of four cases of eosinophilic granuloma were investigated using monoclonal antibodies against Ki-67 proliferation marker (MIB-1) antigen and the TdT mediated dUTP-biotin 3'-OH nick end labelling (TUNEL) method. These data were then compared with the clinical growth rate. Results-Only the Langerhans histiocytic cells, which reacted positively with anti-S-100 protein antibody, were immunolabelled with antibodies to proliferating cell nuclear antigen and Ki-67 antigen (MIB-1). Many apoptotic figures of histiocytic cells were also detected in all cases by the TUNEL method. In a patient whose tumour clinically showed spontaneous regression, the TUNEL staining index gave a higher score than the MIB-1 staining index. Conclusions-The main cause ofthe spontaneous regression ofthe tumours was postulated to be programmed cell death (apoptosis). (J Clin Pathol: Mol Pathol 1995;48:M251-M255)

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Expression of Neuron‐specific Enolase Immunoreactivity by Cutaneous and Extracutaneous Langerhans‐cell Histiocytoses (“X”)

Cited by 3 publications

References 24 publications

Functional imaging of Langerhans cell histiocytosis by ¹¹¹In‐DTPA‐D‐Phe¹‐octreotide scintigraphy

Functional imaging of Langerhans cell histiocytosis by ¹¹¹In‐DTPA‐D‐Phe¹‐octreotide scintigraphy

Langerhans’ cell histiocytosis in adults

Proliferative activity and apoptosis of Langerhans histiocytes in eosinophilic granulomas as evaluated by MIB-1 and TUNEL methods

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Expression of Neuron‐specific Enolase Immunoreactivity by Cutaneous and Extracutaneous Langerhans‐cell Histiocytoses (“X”)

Cited by 3 publications

References 24 publications

Functional imaging of Langerhans cell histiocytosis by 111In‐DTPA‐D‐Phe1‐octreotide scintigraphy

Functional imaging of Langerhans cell histiocytosis by 111In‐DTPA‐D‐Phe1‐octreotide scintigraphy

Langerhans’ cell histiocytosis in adults

Proliferative activity and apoptosis of Langerhans histiocytes in eosinophilic granulomas as evaluated by MIB-1 and TUNEL methods

Contact Info

Product

Resources

About

Functional imaging of Langerhans cell histiocytosis by ¹¹¹In‐DTPA‐D‐Phe¹‐octreotide scintigraphy

Functional imaging of Langerhans cell histiocytosis by ¹¹¹In‐DTPA‐D‐Phe¹‐octreotide scintigraphy