Expression of Neuronal Markers in Differentiated Marrow Stromal Cells and CD133+ Stem-Like Cells

Abstract

Bone marrow stromal cells, which normally give rise to bone, cartilage, adipose tissue, and hematopoiesissupporting cells, have been shown to differentiate in vitro and in vivo into neural-like cells. In this study, we examined the expression of neuronal and glial markers in human marrow stromal cells under culture conditions appropriate for neural stem cells, and compared the unsorted cell population to bone marrow CD133 + stem-like cells using immunofluorescence, Western blot, and functional patch-clamp analysis. Overall, the expression of the early neuronal marker β3-tubulin was most pronounced in the presence of DMEM/ F12 and neurotrophin 3 (NT3) or brain-derived neurotrophic factor (BDNF), when marrow stromal cells were cultured onto fibronectin. Electrophysiological examination, however, could not show fast sodium currents or functional neurotransmitter receptors in differentiated marrow stromal cells. CD133+ mesenchymal stem-like cells, but not CD34− cells, generally showed a higher expression of neuronal markers than did unsorted marrow stromal cells, and differentiated CD133+ cells more resembled neuron-like cells.

Synergistic effects of CoCl2 and ROCK inhibition on mesenchymal stem cell differentiation into neuron-like cells

“…Interestingly, media containing low or no serum did not induce MSC morphological changes suggesting that CoCl 2 differentiation required factors present in the serum (data not shown). These results are in accordance with those of Padovan et al (Padovan et al, 2003) showing the influence of fetal bovine serum for neuronal differentiation in the presence of FGF-2. Alternatively, this can be explained by the fact that cell death induced by serum deprivation in both control and CoCl 2 -Journal of Cell Science 119 (13) treated cells might have prevented MSC differentiation.…”

“…Constitutive expression of nestin and Tuj1 by MSCs that we and others (Padovan et al, 2003;Tondreau et al, 2004) observed suggested that these cells have the ability to differentiate into neurons. We hypothesized that HIF-1 activation could trigger MSC differentiation into neuron-like cells.…”

Neurogenic potential of human mesenchymal stem cells revisited: analysis by immunostaining, time-lapse video and microarray

“…Cells with neural characteristics appear to have been generated in vitro from adult stem cells of putative mesodermal origin and isolated from a variety of connective tissues, including bone marrow, umbilical cord blood, dermis and adipose tissue (Hermann et al, 2004;Jin et al, 2003;Kang et al, 2004;Padovan et al, 2003;Sanchez-Ramos et al, 2000;Sanchez-Ramos, 2002;Wislet-Gendebien et al, 2005;WisletGendebien et al, 2003). However, attempts to assert transdifferentiation of adult bone-marrow-derived cells into neural lineages in vivo have produced discordant results.…”

Mesenchymal Stem Cells in CNS Regeneration

“…Chen et al, 2005;Joannides et al, 2003;B. J. Kim, Seo, Bubien, & Oh, 2002; S. S. Kim et al, 2005;Kondo, Johnson, Yoder, Romand, & Hashino, 2005;Lim et al, 2008;Long, Olszewski, Huang, & Kletzel, 2005;Padovan et al, 2003;Sanchez-Ramos et al, 2000;Zeng et al, 2011). Other studies have used different culture media, supplemented with individual or various combinations of chemical and pharmacological agents, such as DMSO, b-mercaptoethanol, 5-bromo-2-deoxyuridine (BrdU), butylated hydroxyanisole, forskolin, and dibutyryl cyclic AMP (Ankeny, McTigue, & Jakeman, 2004;W.…”