Expression of neuronal protein synuclein gamma gene as a novel marker for breast cancer prognosis

Wu, Kangning; Quan, Zhiwei; Weng, Zhen; Li, Fangming; Zhang, Yichu; Yao, Xin; Chen, Yiding; Budman, Daniel R.; Goldberg, Itzhak D.; Shi, Yuye

doi:10.1007/s10549-006-9296-7

Cited by 52 publications

(54 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression of SNCG in breast cancer cells leads to a significant increase in motility and invasiveness in vitro and a profound augmentation of metastasis in nude mice (Jia et al, 1999). Consistent with the ability to confer metastatic potential to breast cancer cells, we recently demonstrated that patients with SNCG-positive breast cancer have statistically higher incidence for metastasis compared with patients with SNCG-negative cancer (Wu et al, 2006). In addition, a recent study also indicates a strong association between SNCG protein expression in primary tumors and distant metastasis in diversified cancer types, including liver, esophagus, colon, gastric, lung, prostate, cervical and breast cancer .…”

Section: Discussionsupporting

confidence: 64%

“…SNCB and SNCG have also been recognized to play a role in the pathogenesis of PD and Lewy bodies cases (Galvin et al, 1999;Duda et al, 2000). Although synucleins are highly expressed in neuronal cells and are abundant in presynaptic terminals, they have also been implicated in non-neural diseases, particularly in the hormone responsive cancers of breast (Ji et al, 1997(Ji et al, , 1999Jiang et al, 2003Jiang et al, , 2004Inaba et al, 2005;Wu et al, 2003Wu et al, , 2006Gupta et al, 2003a, b) and ovary (Lavedan et al, 1998;Bruening et al, 2000;Liu et al, 2000;Lu et al, 2000;Pan et al, 2002;Gupta et al, 2003a).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chaperoning of estrogen receptor and induction of mammary gland proliferation by neuronal protein synuclein gamma

Liu

Jiang

et al. 2006

Oncogene

View full text Add to dashboard Cite

Synucleins are emerging as central players in the formation of pathologically insoluble deposits characteristic of neurodegenerative diseases. However, synuclein c (SNCG), previously identified as a breast cancer specific gene (BCSG1), is also highly associated with breast cancer progression. Using transgenic mouse model, we demonstrated a role of SNCG in induction of highly proliferative pregnancy-like phenotype of mammary epithelial cells and branching morphology. SNCG participated in the heat shock protein-based multiprotein chaperone complex for steroid receptor signaling. Expression of SNCG in mammary epithelium resulted in a significant stimulation of ERa transcriptional activity. SNCG-induced mammary gland proliferation can be effectively blocked by antiestrogen and ovariectomy, indicating that the induced proliferation is mediated by ERa signaling and requires estrogen stimulation. These data indicate the chaperone activity of SNCG on stimulation of steroid receptor signaling in mammary gland and, thus induces extensive mammary gland proliferation and contributes to the hormonal impact on mammary tumorigenesis.

show abstract

Section: Discussionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Chaperoning of estrogen receptor and induction of mammary gland proliferation by neuronal protein synuclein gamma

Liu

Jiang

et al. 2006

Oncogene

View full text Add to dashboard Cite

show abstract

“…SNCG predicts poor clinical outcome in BC (Guo et al, 2007;Wu et al, 2007). When overexpressed, SNCG stimulates growth of hormone-dependent BC cells both in vitro and in nude mice (Jiang et al, 2003;Jiang et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

MiRNA-15a Mediates Cell Cycle Arrest and Potentiates Apoptosis in Breast Cancer Cells by Targeting Synuclein-γ

Xie²,

Chen³

et al. 2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Background: Recent studies have indicated that microRNA-15a (miR-15a) is dysregulated in breast cancer (BC). We aimed to evaluate the expression of miR-15a in BC tissues and corresponding para-carcinoma tissues. We also focused on effects of miR-15a on cellular behavior of MDA-MB-231 and expression of its target gene synuclein-γ (SNCG). Materials and Methods: The expression levels of miR-15a were analysed in BC formalin fixed paraffin embedded (FFPE) tissues by microarray and quantitative real-time PCR. CCK-8 assays, cell cycle and apoptosis assays were used to explore the potential functions of miR-15a in MDA-MB-231 human BC cells. A luciferase reporter assay confirmed direct targets. Results: Downregulation of miR-15a was detected in most primary BCs. Ectopic expression of miR-15a promoted proliferation and suppressed apoptosis in vivo. Further studies indicated that miR-15a may directly interact with the 3'-untranslated region (3'-UTR) of SNCG mRNA, downregulating its mRNA and protein expression levels. SNCG expression was negatively correlated with miR-15a expression. Conclusions: MiR-15a has a critical role in mediating cell cycle arrest and promoting cell apoptosis of BC, probably by directly targeting SNCG. Thus, it may be involved in development and progression of BC.

show abstract

“…have indicated that overexpression of Á-synuclein led to a significant increase in motility and invasiveness in cell culture and to a profound augmentation of metastasis in nude mice (10)(11)(12)(13)24,25). ·-, ß-synuclein were also expressed in some nervous system cancers, and breast and ovarian cancer (17,18).…”

Section: -------------------------------------------------mentioning

confidence: 99%

“…The following studies showed that Á-synuclein was aberrantly expressed in various types of cancer, especially in advanced stages of the diseases, but rarely expressed in tumor-matched non-neoplastic adjacent tissues (NNAT) (10). Patients with Á-synuclein protein-positive breast cancer have a significantly shorter disease-free survival and overall survival period compared to patients with no Á-synuclein expression (11,12). Furthermore, studies to date indicate that overexpression of Á-synuclein can stimulate proliferation, and induce invasion and metastasis of breast cancer cells in vitro assays as well as in animal models (13).…”

Section: Introductionmentioning

confidence: 99%

Expression of α-, β- and γ-synuclein in colorectal cancer, and potential clinical significance in progression of the disease

Wang²,

Peng³

et al. 2009

Oncol Rep

View full text Add to dashboard Cite

Abstract. The synucleins (·-, ß-and Á-synuclein) are a small, soluble, highly conserved group of neuronal proteins that attracted considerable attention due to their involvement in both neurodegenerative diseases and cancer. In this study, we examined the synuclein exprsssion in colorectal cancer (CRC) tissues, tumor-matched non-neoplastic adjacent tissues (NNAT), and CRC cell lines, and then investigated clinical significance of synucleins. By using semi-quantitative RT-PCR, synuclein mRNA expression was detected in eight CRC cell lines. It was much higher in CRC samples than in NNAT samples (P<0.05). The results of Western blotting showed that the levels of synucleins protein expression in CRC cells approximately corresponded to the levels of synuclein mRNA expression. Immunohistochemical staining revealed that Á-synuclein protein expression was up-regulated in CRC samples compared to NNAT samples (P=0.022), and was significantly correlated with clinical stage and lymph node involvement of CRC (P<0.05). Although, there was no significant difference in either ·-or ß-synuclein protein expression between tumor and normal samples (P>0.05), often more than one form of synuclein was expressed in a tumor sample. More ratios of later stage and lymph node-positive tumors expressed a least one type of synuclein protein, and more ratios showed positive for either · or Á-synuclein expression, as well as positive either for ß or Á-synuclein in more ratios of lymph node-positive tumors. These results show that ·-, ß-and Á-synuclein are expressed in a high percentage of CRC. Á-synuclein protein is valuable for evaluation of progression of CRC, and it is more sensitive to predict advanced stage and lymph node invasion by detection of Á-synuclein protein combined with either ·-or ß-synuclein protein or both than by detection of Á-synuclein only. IntroductionThe synucleins are a family of small, soluble, highly conserved neuronal proteins that consist of ·-, ß-, and Á-synuclein. They are a natively unfolded group of proteins that are characterized by 5-6 repeats of amino acid motif (KTKEGV), constituting most of the N-terminal half of the proteins. These repeats result in the formation of conserved amphipathic A2-helices also characteristic of apolipoproteins, which mediate reversible binding to phospholipid membranes. This property supports the role of synucleins in vesicular release at presynaptic nerve terminals (1-4). Although their normal cellular functions have not been clearly defined, synucleins have attracted considerable attention due to their involvement in neurodegenerative diseases. ·-synuclein is the major component of Lewy bodies in Parkinson's disease and has also been identified as the non-amyloid component of amyloid deposition, the hallmark of Alzheimer's disease (5,6). ß-and Á-synuclein are assumed to have a neuroprotective role by inhibiting ·-synuclein aggregation and toxicity (7,8).Synuclein expression is usually highly tissue-specific, and restricted to brain tissue and presynaptic terminals. However, a...

show abstract

Expression of neuronal protein synuclein gamma gene as a novel marker for breast cancer prognosis

Cited by 52 publications

References 23 publications

Chaperoning of estrogen receptor and induction of mammary gland proliferation by neuronal protein synuclein gamma

Chaperoning of estrogen receptor and induction of mammary gland proliferation by neuronal protein synuclein gamma

MiRNA-15a Mediates Cell Cycle Arrest and Potentiates Apoptosis in Breast Cancer Cells by Targeting Synuclein-γ

Expression of α-, β- and γ-synuclein in colorectal cancer, and potential clinical significance in progression of the disease

Contact Info

Product

Resources

About