Expression of NGF, BDNF and their high-affinity receptors in ovine mammary glands during development and lactation

Expression of NGF, BDNF and their receptors in subcutaneous adipose tissue of lactating cows

“…On the other hand, p75NTR transmits both positive and negative signals (Reichardt, 2006). To date, neurotrophins have been mainly studied in nervous cells, but, as multifunctional growth factors, they can exert various effects through their receptors on non-neuronal cells such as testis (Müller et al, 2006), thymus (Maroder et al, 2000), skin (Di Marco et al, 1993), salivary glands and mammary ducts (Shibayama and Koizumi, 1996;Sariola, 2001) and ovine mammary glands (Colitti, 2015). It is noteworthy that, because NGF and BDNF are secreted by WAT and BAT (Sornelli et al, 2009) and their levels in the circulation and tissue are altered in cardiometabolic diseases , neurotrophins are considered as metabotrophic factors, implicating in metabolic diseases (Chaldakov et al, 2010).…”

Proliferation and apoptosis in subcutaneous adipose tissue of lactating cows with different genetic merit for milk yield

“…Immunohistochemical investigations were performed as described more fully by Colitti (2015). The avidin-biotinperoxidase complex (ABC) method was performed using the Vectastain ABC kit (PK-4000, Vector Laboratories, Burlingame, CA, USA).…”

“…Adipose tissue responds to biochemical and nervous signals and it plays an active role during lactation through the secretion of signaling molecules (Colitti, 2015;Hovey and Aimo, 2010). Diet composition and plan of nutrition, parity and milk yield strongly influence the extent of AT accumulation and losses and it has been observed that also genetic merit of the cow can influence the mobilization of fat depots (Sgorlon et al, 2015a).…”

Distribution of BDNF and TrkB isoforms in growing antler tissues of red deer

“…Immunohistochemical analysis was performed according to procedures previously described (Colitti, 2015). Slides were deparaffinised, rehydrated through graded concentrations of alcohol to distilled water and submitted to antigen retrieval with proteinase K (Sigma, Milan, Italy) working solution (20 g/ml) in Tris-EDTA (TE) buffer pH 8.0 at room temperature (RT) for 8 min.…”

“…It was reported in adipocytes , uterus (Wessels et al, 2015), testis (Müller et al, 2006), kidney (Caroleo et al, 2015), keratinocytes (Marconi et al, 2003), salivary and mammary glands (Shibayama and Koizumi, 1996;Colitti, 2015).…”