Expression of NK1 receptor at the protein and mRNA level in the porcine female reproductive system

Bukowski, R.

doi:10.2478/pjvs-2014-0068

Cited by 3 publications

(2 citation statements)

References 35 publications

(36 reference statements)

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“…The primers for the RT-PCR assay could be designed based only on the homology of the NK1 receptor mRNA sequences in different mammalian species. For the porcine NK1 receptor, this method enabled the detection of NK1R transcripts only once in the porcine female reproductive system (Bukowski 2014).…”

Section: Oryctolagus Cuniculus Nk2r Sus Scrofa Nk2rmentioning

confidence: 99%

Characterisation, localisation and expression of porcine TACR1, TACR2 and TACR3 genes

Jakimiuk¹,

Podlasz²,

Chmielewska-Krzesińska³

et al. 2017

Vet. Med.

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Substance P is involved in many physiological and pathophysiological processes. This functional diversity is mediated by three neurokinin receptor subtypes (NK1R, NK2R and NK3R) encoded by the TACR1, TACR2 and TACR3 genes, respectively. Despite the increasing interest in using pigs (Sus scrofa) to study human disease mechanisms, the sequences of these receptors are still unconfirmed or in the case of the NK1 receptor, not yet even unpredicted. We employed in silico analysis to define the localisation of the porcine tachykinin receptor genes, and to predict the structures and amino acid sequences of the respective proteins. A reverse transcription polymerase chain reaction (RT-PCR) assay was performed to analyse the expression of tachykinin receptor genes in different porcine tissues. The data show that the TACR1 gene is located on chromosome 3, TACR2 on chromosome 14 and TACR3 on chromosome 8. All three genes encode proteins with structures that incorporate features of G-protein-coupled receptors with sizes of 407, 381 and 464 amino acids, respectively. The receptors display a high degree of similarity to other mammalian neurokinin receptors. The NK1R subtype is expressed in both the central nervous system and peripheral tissues, while NK2R expression seems to be localised mostly to peripheral tissues. The expression of NK3R is found mainly in the central nervous system. This report provides for the first time the results of a comprehensive analysis of the structure and distribution of porcine NK1R, as well as other porcine neurokinin receptors and their genes. We hope that our data may offer an invaluable foundation for the future studies on the function of diverse tachykinin peptides in the central nervous system and peripheral tissues.

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Section: Oryctolagus Cuniculus Nk2r Sus Scrofa Nk2rmentioning

confidence: 99%

Characterisation, localisation and expression of porcine TACR1, TACR2 and TACR3 genes

Jakimiuk¹,

Podlasz²,

Chmielewska-Krzesińska³

et al. 2017

Vet. Med.

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“…On the other hand, in pigs, NK1R was expressed at the RNA level not only in the uterus, but also in all parts of the female reproductive system [ 25 ]. The presented study showed the expression of NK1R and NK2R in the myometrium in the physiological state and as a result of inflammation.…”

Section: Discussionmentioning

confidence: 99%

Effects of Substance P and Neurokinin A on the Contractile Activity of Inflamed Porcine Uterus

Brzozowska

Romaniewicz

Całka

et al. 2022

IJMS

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Disturbances in uterine contractile activity contribute to the development of inflammation, and recent evidence indicates that tachykinins, including substance P (SP) and neurokinin A (NKA), are involved in controlling uterine function. Here, we determined the effect of Escherichia coli (E. coli)-induced inflammation on expression of protein receptor subtypes for substance P (NK1R) and neurokinin A (NK2R) in the pig myometrium as well as their role in contractility of inflamed uterus. The severe acute endometritis developed in the E. coli group and the expression of NK1R and NK2R proteins increased in the myometrium. Compared to the pre-administration period, SP (10−6 M) reduced the amplitude and frequency in the myometrium of the E. coli group and the amplitude was higher and the frequency was lower versus other groups. NKA reduced the amplitude and increased the frequency in endometrium/myometrium of the E. coli group. In this group, the amplitude was lower and the frequency was higher than in the CON and SAL groups. Our research showed that NK2R (10−6 M) antagonist application abolished the NKA inhibitory effect on uterine amplitude. The application of the NK1R (10−5 M) antagonist together with SP revealed that the inhibitory effect of SP on uterine contractility is achieved independently of the NKR1. Additionally, taking into account the fact that NKA shows an inhibitory effect with the use of NK2R on uterine amplitude suggests the possibility of therapeutic use of the antagonist as a drug increasing uterine contractility in inflammation.

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Potential in vitro therapeutic effects of targeting SP/NK1R system in cervical cancer

et al. 2021

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Expression of NK1 receptor at the protein and mRNA level in the porcine female reproductive system

Cited by 3 publications

References 35 publications

Characterisation, localisation and expression of porcine TACR1, TACR2 and TACR3 genes

Characterisation, localisation and expression of porcine TACR1, TACR2 and TACR3 genes

Effects of Substance P and Neurokinin A on the Contractile Activity of Inflamed Porcine Uterus

Potential in vitro therapeutic effects of targeting SP/NK1R system in cervical cancer

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