Expression of Oct4 in human embryonic stem cells is dependent on nanotopographical configuration

Kong, Ying; Tu, Christina H.; Donovan, Peter; Yee, Albert F.

doi:10.1016/j.actbio.2013.01.036

Cited by 56 publications

(58 citation statements)

References 59 publications

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“…Various nanolithography strategies have been used to control the size, shape, spacing and organizational symmetry of nanometre-scale features in a variety of materials. Electrospinning can form nanofibrous substrates from natural or synthetic polymer precursors 113 , and nanolithography methods common in the microelectronics industry (such as electron-beam lithography, block-copolymer assembly, selective etching or nano-imprint lithography) can generate nanopits 3,4 , nanopillars 114 or nanochannels 115 on various surfaces. The diameter and spacing of electrospun nanofibres or nanochannels can be varied in ranges that approach the dimensions of natural basement-membrane fibre sizes (5–200 nm) and pore sizes (3–80 nm; ref.…”

Section: Box 1 Features Of Stem Cell Substrate Materialsmentioning

confidence: 99%

“…Channels and pillars formed via lithographic techniques can also be varied in ranges that mimic the porosity of natural ECM. For example, nanopillars 30 nm in diameter arranged in hexagonal or honeycomb arrangements supported human embryonic stem cell (hESC) self-renewal 114 , and nanopits 120 nm in diameter with 300 nm centre-to-centre spacing could support hMSC expansion in a symmetric configuration 4 , and osteogenesis when organized asymmetrically 3 (Fig. 2b).…”

Section: Box 1 Features Of Stem Cell Substrate Materialsmentioning

confidence: 99%

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Materials as stem cell regulators

2014

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The stem cell/material interface is a complex, dynamic microenvironment in which the cell and the material cooperatively dictate one another's fate: the cell by remodelling its surroundings, and the material through its inherent properties (such as adhesivity, stiffness, nanostructure or degradability). Stem cells in contact with materials are able to sense their properties, integrate cues via signal propagation and ultimately translate parallel signalling information into cell fate decisions. However, discovering the mechanisms by which stem cells respond to inherent material characteristics is challenging because of the highly complex, multicomponent signalling milieu present in the stem cell environment. In this Review, we discuss recent evidence that shows that inherent material properties may be engineered to dictate stem cell fate decisions, and overview a subset of the operative signal transduction mechanisms that have begun to emerge. Further developments in stem cell engineering and mechanotransduction are poised to have substantial implications for stem cell biology and regenerative medicine.

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Section: Box 1 Features Of Stem Cell Substrate Materialsmentioning

confidence: 99%

Section: Box 1 Features Of Stem Cell Substrate Materialsmentioning

confidence: 99%

Materials as stem cell regulators

2014

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“…In human ES cells, nanopatterned substrates that downregulate cell adhesion upregulate ES self-renewal properties (Tables 1 and 2). 150 Similarly, in mouse ES cells, nanopatterned substrates that downregulate cell adhesion and spreading upregulate ES self-renewal properties; these effects have been shown to result from integrin-mediated FAK signaling to suppress the ERK/JNK activity (Tables 1 and 2). In contrast, mouse ES cells with upregulated stress fiber formation and cell adhesion exhibit enhanced mesendoderm differentiation (Table 1).…”

Section: Stem Cell Differentiationmentioning

confidence: 99%

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

Miyoshi

Adachi

2014

Tissue Engineering Part B: Reviews

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“…Indeed, current biomaterial fabrication technologies not only achieve structural support, but also maintain permanently differentiated cell phenotype and/or direct lineage commitment of stem cells. For example, topographical features have been shown to maintain Oct4 expression in human embryonic stem cell culture, even in the absence of basic fibroblast growth factor supplementation [14]; topographical cues alone [15,16] or in combination with neurotrophic signals [17] have also been shown to enhance contact guidance and neuronal differentiation of human neural stem cells in vitro.…”

Section: Introductionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractControlling the cell-substrate interactions at the bio-interface is becoming an inherent element in the design of implantable devices. Modulation of cellular adhesion in vitro, through topographical cues, is a well-documented process that offers control over subsequent cellular functions. However, it is still unclear whether surface topography can be translated into a clinically functional response in vivo at the tissue / device interface. Herein, we demonstrated that anisotropic substrates with a groove depth of ~317 nm and ~1,988 nm promoted human tenocyte alignment parallel to the underlying topography in vitro. However, the rigid poly(lactic-co-glycolic acid) substrates used in this study upregulated the expression of chondrogenic and osteogenic genes, indicating possible tenocyte trans-differentiation. Of significant importance is that none of the topographies assessed (~37 nm, ~317 nm and ~1,988 nm groove depth) induced extracellular matrix orientation parallel to the substrate orientation in a rat patellar tendon model. These data indicate that two-dimensional imprinting technologies are useful tools for in vitro cell phenotype maintenance, rather than for organised neotissue formation in vivo, should multifactorial approaches that consider both surface topography and substrate rigidity be established.

show abstract

Expression of Oct4 in human embryonic stem cells is dependent on nanotopographical configuration

Cited by 56 publications

References 59 publications

Materials as stem cell regulators

Materials as stem cell regulators

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

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