Expression of ocular albinism 1 (OA1), 3, 4- dihydroxy- L-phenylalanine (DOPA) receptor, in both neuronal and non-neuronal organs

L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor

“…infusion of phenylephrine, an ADRA1 agonist, in Gpr143 -/y mice (ref. 12 and Figure 1A). The pressor response to phenylephrine in itself was attenuated in Gpr143 -/y mice compared with WT mice (Figure 1, B and C).…”

“…Male littermates mice were used in the present study. Gpr143 -/y and Gpr143 fl mice were established as described previously (12). Mice lacking GPR143 in VSMCs were generated by mating female Gpr143 +/fl and male SM22-cre mice.…”

“…Following this process, sections were blocked for 1 hour in Triton-TBS (137 mM NaCl, 2.68 mM KCl, 25 mM Tris base, 0.1 % Triton X-100 [TBST]) containing 10% normal goat serum (NGS). The sections were incubated with anti-TH (#22941, ImmunoStar) antibody, anti-GPR143 antibody (12), or anti-ADRA1B (#AAR-018, Alomone Labs) antibody at 4°C for 24 hours. Antibody was incubated with antigen at 37°C for 30 minutes for pre-absorption test.…”

“…Therefore, to further determine whether GPR143 plays a physiological role, we examined peripheral vasomotor response to i.v. administration of phenylephrine, a nonselective adrenergic receptor, alpha1 (ADRA1) agonist, in GPR143 gene-deficient (Gpr143 -/y ) mice (12). We found that the pressor response to phenylephrine was markedly attenuated in Gpr143 -/y mice compared with Gpr143 +/y (WT) mice.…”

“…GPR143 is also expressed in non-neuronal organs receiving sympathetic innervation, such as the heart and blood vessels (12), thereby suggesting its involvement in peripheral cardiovascular regulation. Therefore, to further determine whether GPR143 plays a physiological role, we examined peripheral vasomotor response to i.v.…”