Expression of olfactory receptors in the cribriform mesenchyme during prenatal development

Schwarzenbacher, Karin; Fleischer, Joerg; Breer, Heinz; Conzelmann, Sidonie

doi:10.1016/j.modgep.2004.02.004

Cited by 35 publications

(48 citation statements)

References 19 publications

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“…5E). These results exactly reflected the features for the extraepithelial cells expressing mOR256 -17, as previously obtained by analysis of olfactory marker protein-GFP transgenic animals (Schwarzenbacher et al, 2004). These observations further support the view that the anti-mOR256 -17 antibody specifically recognized the mOR256 -17 protein.…”

Section: Resultssupporting

confidence: 87%

“…For the receptor type mOR256 -17 we have recently demonstrated that mRNA is not only detectable in cells of the olfactory epithelium, but, during distinct developmental stages, also in cells positioned within the cribriform mesenchyme, i.e., between the olfactory epithelium and the developing olfactory bulb (Schwarzenbacher et al, 2004). To evaluate whether the antimOR256 -17 antibody may also stain these "extraepithelial" cells, comparative labeling procedures were performed.…”

Section: Resultsmentioning

confidence: 99%

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Olfactory Receptor Proteins in Axonal Processes of Chemosensory Neurons

Strotmann

Lévai²,

Fleischer³

et al. 2004

J. Neurosci.

129

119

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Olfactory receptors are supposed to act not only as molecular sensors for odorants but also as cell recognition molecules guiding the axons of olfactory neurons to their appropriate glomerulus in the olfactory bulb. This concept implies that olfactory receptor proteins are located in sensory cilia and in the axons. To approach this critical issue, antibodies were generated against two peptides, one derived from olfactory receptor mOR256 -17, one derived from the "mOR37" subfamily. By means of immunohistochemistry and double-labeling studies using transgenic mouse lines as well as Western blot analyses, it was demonstrated that the newly generated antibodies specifically recognized the receptor proteins. To scrutinize the hypothesis that olfactory receptor proteins may also be present in the axonal processes and the nerve terminals, serial sections through the olfactory bulb were probed with the antibodies. Two glomeruli in each bulb were stained by anti-mOR256 -17, one positioned in the medial, one in the lateral hemisphere. Fiber bundles approaching the glomeruli through the outer nerve layer also displayed intense immunofluorescence. A similar picture emerged for the antibody anti-mOR37, a small number of glomeruli in the ventral domain of the bulb was stained. On serial sections through the olfactory bulb of mOR37-transgenic mouse lines, double-labeling experiments demonstrated that distinct immunoreactive glomeruli corresponded to glomeruli that were targeted by neurons expressing a particular member of the mOR37 receptor subfamily. These data indicate that olfactory receptor (OR) proteins are indeed present in the axonal processes and nerve terminals of olfactory sensory neurons, thus supporting the notion that ORs may participate in the molecular processes underlying the fasciculation and targeting of olfactory axons.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Olfactory Receptor Proteins in Axonal Processes of Chemosensory Neurons

Strotmann

Lévai²,

Fleischer³

et al. 2004

J. Neurosci.

129

119

View full text Add to dashboard Cite

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“…A series of studies in the 1990s revealed that the GnRH cells are just one of several components of such migrating cells, and that an astonishing variety of immuno-or lectin binding properties exist among non-GnRH cells that follow similar migratory routes from the olfactory epithelium towards the olfactory bulb. Such phenotypes include olfactory marker protein (OMP, Conzelmann et al, 2002;Schwarzenbacher et al, 2004;Valverde et al, 1993), NPY (Hilal et al, 1996), GABA (Tobet et al, 1996a), AChE (DeCarlos et al, 1995), beta-tubulin (DeCarlos et al, 1995), B50/growth-associated protein (GAP43, , carnosine (Tarozzo et al, 1994), neuron-specific enolase (NSE, , somatostatin (Murakami and Arai, 1994b), tyrosine hydroxylase (TH, Halpern-Sebold et al, 1985;Verney et al, 1996), FMRF-amide (Fiorentino et al, 2001;Yamamoto et al, 1996), the homeoprotein OTX2 (Mallamaci et al, 1996), neural cell adhesion molecule (NCAM, Toba et al, 2001), and calbindin (Toba et al, 2001). These reports are summarized in Table 3.…”

Section: Migration Of Non-gnrh Presumptive Nervus Terminalis Componentsmentioning

confidence: 99%

“…These reports are summarized in Table 3. While some of these molecules (such as FMRF, NPY, or TH) may be coexpressed with GnRH in some species (Hilal et al, 1996;Stell et al, 1984;Verney et al, 1996; but see Yamamoto et al, 1996), there appears to be no or only very little overlap in the expression of OMP, carnosine, somatostatin, and calbindin with GnRH expression (Murakami and Arai, 1994b;Schwarzenbacher et al, 2004;Tarozzo et al, 1994;Toba et al, 2001). Similar cells containing olfactory receptor proteins also do not co-express GnRH (Strotmann et al, 2004;J.…”

Section: Migration Of Non-gnrh Presumptive Nervus Terminalis Componentsmentioning

confidence: 99%

“…Lack of such expression of peptides could be the result of temporary or intermittent expression of these markers, as has been discussed for lizards (D'Aniello et al, 1994;Masucci et al, 1992). However, studies in the 1980s and 1990s demonstrated that the "olfactory" markers used to reveal the identity of extrabulbar projections as primary olfactory, in fact, were not specific for olfactory receptor neurons in the olfactory epithelium, but also labeled bona fide nervus terminalis components (Alonso et al, 1989;Stell et al, 1985;Szabo et al, 1991b;Tobet et al, 1996a) or cell types that migrate from the olfactory epithelium just like GnRH-expressing terminalis cells (Murakami and Arai, 1994b;Schwarzenbacher et al, 2004;Valverde et al, 1993;Verney et al, 1996). Such facts cast serious doubt on the notion that extrabulbar projections identified by olfactory markers necessarily distinguish the projections as primary olfactory and originating from true olfactory receptor neurons rather than being components of the nervus terminalis system.…”

Section: Introductionmentioning

confidence: 98%

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The terminal nerve and its relation with extrabulbar “olfactory” projections: Lessons from lampreys and lungfishes

Bartheld

2004

Microscopy Res & Technique

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The definition of the terminal nerve has led to considerable confusion and controversy. This review analyzes the current state of knowledge as well as diverging opinions about the existence, components, and definition of terminal nerves or their components, with emphasis on lampreys and lungfishes. I will argue that the historical terminology regarding this cranial nerve embraces a definition of a terminal nerve that is compatible with its existence in all vertebrate species. This review further summarizes classical and more recent anatomical, developmental, neurochemical, and molecular evidence suggesting that a multitude of terminalis cell types, not only those expressing gonadotropin-releasing hormone, migrate various distances into the forebrain. This results in numerous morphological and neurochemically distinct phenotypes of neurons, with a continuum spanning from olfactory receptor-like neurons in the olfactory epithelium to typical large ganglion cells that accompany the classical olfactory projections. These cell bodies may lose their peripheral connections with the olfactory epithelium, and their central projections or cell bodies may enter the forebrain at several locations. Since "olfactory" marker proteins can be expressed in bona fide nervus terminalis cells, so-called extrabulbar "olfactory" projections may be a collection of disguised nervus terminalis components. If we do not allow this pleiomorphic collection of nerves to be considered within a terminal nerve framework, then the only alternative is to invent a highly species- and stage-specific, and, ultimately, thoroughly confusing nomenclature for neurons and nerve fibers that associate with the olfactory nerve and forebrain.

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Spatial Expression of Otolith Matrix Protein‐1 and Otolin‐1 in Normally and Kinetotically Swimming Fish

Weigele

Franz‐Odendaal

Hilbig

2015

The Anatomical Record

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Kinetosis (motion sickness) has been repeatedly shown to affect some fish of a given clutch following the transition from 1g to microgravity or from hypergravity to 1g. This susceptibility to kinetosis may be correlated with irregular inner ear otolith growth. Otoliths are mainly composed of calcium carbonate and matrix proteins, which play an important role in the process of otolith mineralization. Here, we examine the morphology of otoliths and the expression pattern of the major otolith proteins OMP-1 and otolin-1 in a series of hypergravity experiments. In the utricle, OMP-1 is present in centripetal (medial) and centrifugal (lateral) regions of the meshwork area. In the saccule, OMP-1 was expressed within a dorsal and a ventral narrow band of the meshwork area opposite to the periphery of the sulcus acusticus. In normal animals, the spatial expression pattern of OMP-1 reaches more posteriorly in the centrifugal aspect and is considerably broader in the centripetal portion of the utricle compared to kinetotic animals. However, otolin-1 was not expressed in the utricule. In the saccule, no differences were observed for either gene when comparing normal and kinetotically behaving fish. The difference in the utricular OMP-1 expression pattern between normally and kinetotically swimming fish indicates a different otolith morphology and thus a different geometry of the otoliths resting on the corresponding sensory maculae. As the utricle is the endorgan responsible for sensing gravity, the aberrant morphology of the utricular otoliths, based on OMP-1 expression, likely leads to the observed kinetotic behavior. Anat Rec, 298:1765Rec, 298: -1773Rec, 298: , 2015. V C 2015 Wiley Periodicals, Inc.

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Expression of olfactory receptors in the cribriform mesenchyme during prenatal development

Cited by 35 publications

References 19 publications

Olfactory Receptor Proteins in Axonal Processes of Chemosensory Neurons

Olfactory Receptor Proteins in Axonal Processes of Chemosensory Neurons

The terminal nerve and its relation with extrabulbar “olfactory” projections: Lessons from lampreys and lungfishes

Spatial Expression of Otolith Matrix Protein‐1 and Otolin‐1 in Normally and Kinetotically Swimming Fish

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