Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System

Jung, Hae Sun; Hwang, In Wook; Kim, Su Mi; Kim, Chul-Joong; Shin, Kihyun; Kim, Hyun Soo

doi:10.4142/jvs.2002.3.1.13

Cited by 6 publications

(4 citation statements)

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“…To date, researchers all over the world have contributed various forms of vaccines utilizing different strategies targeting these pathogens. Virus-based vectored vaccines were developed using modified vaccinia virus Ankara, canine adenovirus-2, pseudorabies virus, and Semliki Forest virus [ 124 , 125 , 126 , 127 ]. However, these vectors are of mammalian origin and pose a biohazard threat.…”

Section: Recombinant Baculovirus Display Of Other Viral Proteins Amentioning

confidence: 99%

Baculovirus Surface Display of Immunogenic Proteins for Vaccine Development

Balraj

Wee

Prabakaran

2018

Viruses

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Vaccination is an efficient way to prevent the occurrence of many infectious diseases in humans. To date, several viral vectors have been utilized for the generation of vaccines. Among them, baculovirus—categorized as a nonhuman viral vector—has been used in wider applications. Its versatile features, like large cloning capacity, nonreplicative nature in mammalian cells, and broad tissue tropism, hold it at an excellent position among vaccine vectors. In addition to ease and safety during swift production, recent key improvements to existing baculovirus vectors (such as inclusion of hybrid promoters, immunostimulatory elements, etc.) have led to significant improvements in immunogenicity and efficacy of surface-displayed antigens. Furthermore, some promising preclinical results have been reported that mirror the scope and practicality of baculovirus as a vaccine vector for human applications in the near future. Herein, this review provides an overview of the induced immune responses by baculovirus surface-displayed vaccines against influenza and other infectious diseases in animal models, and highlights the strategies applied to enhance the protective immune responses against the displayed antigens.

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Section: Recombinant Baculovirus Display Of Other Viral Proteins Amentioning

confidence: 99%

Baculovirus Surface Display of Immunogenic Proteins for Vaccine Development

Balraj

Wee

Prabakaran

2018

Viruses

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“…Therefore, a new generation of vaccines with higher safety and protective efficacy is required to control this disease. GP5, which is associated with the development of neutralizing antibodies and protection, has been a leading target for vaccine design [8][9][10][11][12][13][14][15]. Several experimental vaccines based on GP5 have been developed and DNA immunization has been shown to be a promising strategy for controlling PRRS [8-10, 13, 14].…”

Section: Introductionmentioning

confidence: 99%

Enhanced immunogenicity of the modified GP5 of porcine reproductive and respiratory syndrome virus

et al. 2006

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The ORF5-encoded major envelope glycoprotein (GP5) is one of the key immunogenic proteins of the porcine reproductive and respiratory syndrome virus (PRRSV) and is the leading target for the development of the new generation of vaccines against PRRS. However, weak and tardy neutralizing antibodies have been elicited in several developed experimental vaccines expressing PRRSV GP5. More recent evidence has demonstrated a non-neutralizing decoy epitope upstream of the neutralizing epitope of GP5, which might prevent the development of a strong neutralizing antibody response against PRRSV. In the present study, we modified the ORF5 gene by inserting a Pan DR T-helper cell epitope (PADRE) between the neutralizing epitope and the decoy epitope to minimize or eliminate the decoy effect of the non-neutralizing epitope. The immunogenicity of the modified GP5 was further evaluated using DNA vaccination. The results showed that significantly enhanced neutralizing antibodies were elicited in mice immunized with the DNA construct expressing the modified GP5 compared with the native GP5. Slightly increased levels of GP5-specific ELISA antibodies and T-cell proliferative activities were also observed. These results indicate that the high immunogenicity of the modified GP5 might facilitate the development of improved PRRS vaccines in the future.

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“…Among these vaccines, subunit vaccines have been proven to be safer and more effective than conventional vaccines (inactivated and live attenuated vaccines) (21). Recombinant PRRSV proteins have been expressed in several systems, such as E. coli (22), baculovirus (23~25), and Semliki forest virus (26). In the baculovirus expression system (BVES), the model virus is Autographa californica nuclear polyhedrosis virus (AcNPV), which was first isolated from the alfalfa looper (Autographa californica).…”

Section: Introductionmentioning

confidence: 99%

Sequence Analysis and Expression of ORF5 in Korean Isolate of Porcine Reproductive and Respiratory Syndrome Virus

Sun

Kim

et al. 2011

J Bacteriol Virol

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Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of reproductive failure and respiratory disorders in pigs. The viral genome consists of eight overlapping open reading frames (ORFs). ORF5 encodes one of the major glycoproteins and is known as an immunologically important structural protein associated with virus neutralization. The ORF5 gene of the Korean PRRSV isolate, CNV-1, was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and sequenced. The nucleotide and amino acid sequences of CNV-1 ORF5 shared 91% and 83% identity, respectively, with the American isolate (VR2332 strain) and 57% and 49% identity with the European isolate. For the expression and easy purification of ORF5, the cDNA containing the complete ORF5 sequence fused in-frame with sequence encoding glutathione S-transferase (GST) was cloned into a baculovirus transfer vector and transfected into Sf9 cells. The GST-ORF5 fusion protein produced in Sf9 cells was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. Sequencing results confirmed that the recombinant baculovirus from Sf9 cells contains the complete ORF5 gene. Further studies in this direction will address whether ORF5 can be a good candidate for a subunit vaccine against PRRSV in Korea.

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Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System

Cited by 6 publications

References 0 publications

Baculovirus Surface Display of Immunogenic Proteins for Vaccine Development

Baculovirus Surface Display of Immunogenic Proteins for Vaccine Development

Enhanced immunogenicity of the modified GP5 of porcine reproductive and respiratory syndrome virus

Sequence Analysis and Expression of ORF5 in Korean Isolate of Porcine Reproductive and Respiratory Syndrome Virus

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